Effects of Arbuscular Mycorrhizal Fungi on Artemisia annua L. Growth and Chemical Composition of Root Exudates

doi:10.3969/j.issn.1000-2561.2020.09.016

Abstract

Abstract:

The effects of arbuscular mycorrhizal fungi (AMF) Glomus mosseae on the growth index and the root exudates chemical composition of Artemisia annua L. were studied under potted conditions. The results showed that the height, shoot dry weight and root dry weight of A. annua inoculated with AMF increased significantly, with an increase of more than 20%; in addition to root radius, root length, root surface area, root volume, tips, forks and root activity of A. annua increased by 87.0%, 97.0%, 10.7%, 38.4%, 75.6% and 19.6%, respectively; compared with the root exudates obtained from the matrix of A. annua without AMF inoculation (NM-S), the content of soluble protein, soluble sugar and free amino acid in the root exudates obtained from matrix of A. annua with AMF inoculation (AM-S) increased by 74.38%, 16.13% and 203%, respectively, following the types and contents of organic acids increased significantly; but the effects of AMF on the root exudates of hydroponic solution showed an opposite trend. In the substrate, AMF may improve root morphology, root activity and secretion of the root exudates so that A. annua could absorb more nutrients and improve its biomass.

Key words: arbuscular mycorrhizal fungi, Artemisia annua L., root exudates

CLC Number:

S567.2

SUN Chenyu,ZENG Yanhong,MA Junqing,LIU Lu,WANG Wenqi,HUANG Jinghua. Effects of Arbuscular Mycorrhizal Fungi on Artemisia annua L. Growth and Chemical Composition of Root Exudates[J]. Chinese Journal of Tropical Crops, 2020, 41(9): 1831-1837.

Figures/Tables 5

References 28

[1]	Inderjit, Wardle D A, Karban R, et al. The ecosystem and evolutionary contexts of allelopathy[J]. Trends in Ecology and Evolution, 2011,26(12):655-662. DOI URL PMID
[2]	Fernandez C, Monnier Y, Santonja M, et al. The impact of competition and allelopathy on the trade-off between plant defense and growth in two contrasting tree species[J]. Frontiers in Plant Science, 2016,7:594. DOI URL PMID
[3]	Zheng Y L, Feng Y L, Zhang L K, et al. Integrating novel chemical weapons and evolutionarily increased competitive ability in success of a tropical invader[J]. New Phytologist, 2015,205(3):1350-1359. DOI URL PMID
[4]	Efferth T. From ancient herb to modern drug: Artemisia annua and artemisinin for cancer therapy[J]. Seminars in Cancer Biology, 2017,46:65-83. DOI URL PMID
[5]	Li J, Feng W, Lu H, et al. Artemisinin inhibits breast cancer‐induced osteolysis by inhibiting osteoclast formation and breast cancer cell proliferation[J]. Journal of Cellular Physiology, 2019,234(8):12663-12675. DOI URL PMID
[6]	Whiteside M D, Digman M A, Gratton E, et al. Organic nitrogen uptake by arbuscular mycorrhizal fungi in a boreal forest[J]. Soil Biology and Biochemistry, 2012,55:7-13. DOI URL
[7]	Wipf D, Krajinski F, Tuinen D V, et al. Trading on the arbuscular mycorrhiza market: From arbuscules to common mycorrhizal networks[J]. The New Phytologist, 2019,223(3):1127-1142. DOI URL PMID
[8]	Lenoir I, Fontaine J, Lounès-Hadj Sahraoui A. Arbuscular mycorrhizal fungal responses to abiotic stresses: A review[J]. Phytochemistry, 2016,123:4-15. URL PMID
[9]	Parihar M, Meena V S, Mishra P K, et al. Arbuscular mycorrhiza: A viable strategy for soil nutrient loss reduction[J]. Archives of Microbiology, 2019,201(6):723-735. DOI URL PMID
[10]	Stefanowicz A M, Zubek S, Stanek M, et al. Invasion of Rosa rugosa induced changes in soil nutrients and microbial communities of coastal sand dunes[J]. Science of the Total Environment, 2019,677:340-349. DOI URL PMID
[11]	Chen M, Yang G, Sheng Y, et al. Glomus mosseae inoculation improves the root system architecture, photosynthetic efficiency and flavonoids accumulation of liquorice under nutrient stress[J]. Frontiers in Plant Science, 2017,8:931. DOI URL PMID
[12]	黄京华, 谭钜发, 揭红科, 等. 丛枝菌根真菌对黄花蒿生长及药效成分的影响[J]. 应用生态学报, 2011,22(6):1443-1449.
[13]	刘润进, 李晓林. 丛枝菌根及其应用[M]. 北京: 科学出版社, 2000.
[14]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[15]	曾燕红. 丛枝菌根真菌共生条件下黄花蒿根系及其分泌物化感作用研究[D]. 南宁: 广西大学, 2015.
[16]	Laliberté E. Below-ground frontiers in trait-based plant ecology[J]. New Phytologist, 2017,213(4):1597-1603. DOI URL PMID
[17]	Mallik A U, Biswas S R, Collier L C S. Belowground interactions between Kalmia angustifolia and Picea mariana: roles of competition, root exudates and ectomycorrhizal association[J]. Plant and Soil, 2016,403:471-483. DOI URL
[18]	Bardgett R D, Mommer L, De Vries F T. Going underground: Root traits as drivers of ecosystem processes[J]. Trends in Ecology and Evolution, 2014,29(12):692-699. DOI URL PMID
[19]	Baetz U, Martinoia E. Root exudates: the hidden part of plant defense[J]. Trends in Plant Science, 2014,19(2):90-98. DOI URL
[20]	Tsunoda T, van Dam N M. Root chemical traits and their roles in belowground biotic interactions[J]. Pedobiologia, 2017,65:58-67. DOI URL
[21]	Gealy D R, Moldenhauer K A K, Duke S. Root distribution and potential interactions between allelopathic rice, sprangletop (Leptochloa spp.), and barnyardgrass (Echinochloa crus-galli) based on ¹³C isotope discrimination analysis[J]. Journal of Chemical Ecology, 2013,39(2):186-203. DOI URL
[22]	Zhu X C, Skoneczny D, Weidenhamer J D, et al. Identification and localization of bioactive naphthoquinones in the roots and rhizosphere of Paterson’s curse (Echium plantagineum), a noxious invader[J]. Journal of Experimental Botany, 2016,67(12):3777-3788. DOI URL PMID
[23]	Feng G, Zhang F, Li X, et al. Improved tolerance of maize plants tosalt stress by arbuscular mycorrhiza is related to higher accumulation of soluble sugars in roots[J]. Mycorrhiza, 2002,12(4):185-190. DOI URL PMID
[24]	包静. 盐胁迫对黄瓜根系分泌物及土壤微生物的影响[D]. 哈尔滨: 东北农业大学, 2009.
[25]	Ohwaki Y, Hirata H. Differences in carboxylic acid exudation among p-starved leguminous crops in relation to carboxylic acid contents in plant tissues and phospholipid level in roots[J]. Soil Science and Plant Nutrition, 1992,38(2):235-243. DOI URL
[26]	Dinkelaker B, Romheld V, Marschner H. Citric acid excretion and precipitation of calcium citrate in rhizosphere of white lupin (Lupinus albus L.)[J]. Plant, Cell and Environment, 1989,12(3):285-292. DOI URL
[27]	郭修武, 李坤, 郭印山, 等. 丛枝菌根真菌对连作土壤中葡萄生长及根系分泌特性的影响[J]. 沈阳农业大学学报, 2009,40(4):392-395.
[28]	刘进法, 王鹏, 罗园, 等. 低磷胁迫下AM真菌对枳实生苗吸磷效应及根系分泌有机酸的影响[J]. 亚热带植物科学, 2010,39(1):9-13.

项目 Item	株高 Height /cm	苗干重 Shoot DW/g	根干重 Root DW/g	根长 Root length /cm	根表面积 Root area /cm²	根直径 Root diameter/mm	根体积 Root volume /cm³	根尖数 Tips	根分叉数 Forks	根系活力 Root activity /(μg·g^-1·h^-1)
NM	64.9	2.39	0.93	2 954.5	205.6	0.22	2.14	4 243	20 394	33.16
AM	78.0^**	3.53^*	1.45^**	5 526.5^**	405.0^**	0.23	2.37^**	5 871^**	35 822^**	39.66^**
CV/%	±2.9	±15.6	±11.8	±16.1	±14.6	±4.4	±15.0	±4.1	±12.4	±13.8

项目 Item	株高 Height /cm	苗干重 Shoot DW/g	根干重 Root DW/g	根长 Root length /cm	根表面积 Root area /cm²	根直径 Root diameter/mm	根体积 Root volume /cm³	根尖数 Tips	根分叉数 Forks	根系活力 Root activity /(μg·g^-1·h^-1)
NM	64.9	2.39	0.93	2 954.5	205.6	0.22	2.14	4 243	20 394	33.16
AM	78.0^**	3.53^*	1.45^**	5 526.5^**	405.0^**	0.23	2.37^**	5 871^**	35 822^**	39.66^**
CV/%	±2.9	±15.6	±11.8	±16.1	±14.6	±4.4	±15.0	±4.1	±12.4	±13.8

样品 Samples	乳酸 Lactic acid /(μL·L^-1)	乙酸 Acetic acid /(μL·L^-1)	甲酸 Formic acid /(μL·L^-1)	苹果酸 Malic acid /(mg·L^-1)	酒石酸 Tartaric acid /(mg·L^-1)	草酸 Oxalic acid /(mg·L^-1)	柠檬酸 Citric acid /(mg·L^-1)
NM-W	16.53±0.31	0.45±0.01	1.52±0.02	10.33±0.14	2.56±0.05	72.36±0.77	18.91±0.04
AM-W	-	-	1.17±0.01^**	4.30±0.04^**	1.97±0.05^**	67.65±0.18^**	9.97±0.02^**
NM-S	8.13±0.16	-	1.41±0.07	-	-	4.78±0.08	-
AM-S	13.23±0.40^**	0.40±0.04	1.83±0.10^**	-	-	8.92±0.21^**	-

样品 Samples	乳酸 Lactic acid /(μL·L^-1)	乙酸 Acetic acid /(μL·L^-1)	甲酸 Formic acid /(μL·L^-1)	苹果酸 Malic acid /(mg·L^-1)	酒石酸 Tartaric acid /(mg·L^-1)	草酸 Oxalic acid /(mg·L^-1)	柠檬酸 Citric acid /(mg·L^-1)
NM-W	16.53±0.31	0.45±0.01	1.52±0.02	10.33±0.14	2.56±0.05	72.36±0.77	18.91±0.04
AM-W	-	-	1.17±0.01^**	4.30±0.04^**	1.97±0.05^**	67.65±0.18^**	9.97±0.02^**
NM-S	8.13±0.16	-	1.41±0.07	-	-	4.78±0.08	-
AM-S	13.23±0.40^**	0.40±0.04	1.83±0.10^**	-	-	8.92±0.21^**	-