Exogenous Betaine Modulating the Tolerance of Osmotic Stress in Watermelon Cells
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (9): 1816-1821.DOI: 10.3969/j.issn.1000-2561.2020.09.014

• Plant Cultivation, Physiology & Biochemistry • Previous Articles     Next Articles

Exogenous Betaine Modulating the Tolerance of Osmotic Stress in Watermelon Cells

WANG Shihao1,ZHU Fangming1,SUN Mengli1,XU Zijian1,JIANG Xuefei1,*(),QIAO Fei2   

  1. 1. College of Horticulture, Hainan University / Hainan Key Laboratory of Sustainable Utilization of Tropical Biological Resources, Haikou, Hainan 570228, China
    2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministy of Agriculture and Rural Affairs Danzhou, Hainan 571737, China
  • Received:2019-09-19 Revised:2019-12-25 Online:2020-09-25 Published:2020-10-16
  • Contact: JIANG Xuefei

Abstract:

In order to explore the influence of exogenous glycine betaine on watermelon cell growth under osmotic stress and determine the correlation between the glycine betaine and the watermelon’s osmotic stress resistance, measurement of fresh cell weight, cell volume, intracellular reactive oxygen species (ROS) and extracellular pH was performed with the suspension cultured cells of diploid watermelon as the experimental materials, the mannitol simulating the osmotic stress and the exogenous glycine betaine being applied simultaneously. Under osmotic stress, the fresh weight and growth weight of watermelon cells were both inhibited, and extracellular alkalization and burst of intracellular ROS were also induced. The exogenous glycine betaine was found to be able to alleviate the inhibition effect of osmotic stress on the growth weight of the suspension cultured cells of watermelon to a certain degree, and be of regulative effect on extracellular pH and intracellular ROS level. In a word, the exogenous glycine betaine could maintain the watermelon cell growth and regulate the resistant reaction under osmotic stress.

Key words: watermelon, osmotic stress, betaine, extracellular pH, ROS

CLC Number: