Developmental Anatomy of Flower Apparatus of Male Sterile Plant of <i>Camellia vietnamensis</i>
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (9): 1808-1815.DOI: 10.3969/j.issn.1000-2561.2020.09.013

• Plant Cultivation, Physiology & Biochemistry • Previous Articles     Next Articles

Developmental Anatomy of Flower Apparatus of Male Sterile Plant of Camellia vietnamensis

JIA Zechong1,LIU Yunhao1,CHEN Xia1,WANG Hai1,DENG Yajie1,HU Xinwen1,ZHOU Yang2,PANG Zhenzhen2,LAI Hanggui1,*()   

  1. 1. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    2. College of Horticulture, Hainan University, Haikou, Hainan 570228, China
  • Received:2019-10-16 Revised:2020-01-08 Online:2020-09-25 Published:2020-10-16
  • Contact: LAI Hanggui

Abstract:

The flowering sterility of Camellia vietnamensis is common in Hainan, which would seriously affect the production level of Camellia oil. In this study, the normal and sterile plants of C. vietnamensis in Hainan were used as the experimental materials, the external morphological characteristics of flower bud development were compared and observed, and the differences of pollen germination and pollen tube development were detected by the pollen in situ germination technique. The paraffin section method was used to observe the pistil and stamen during flower bud differentiation, and the correlation between the period of pistil and stamen development and the external morphology of flower bud was analyzed. In this study, the causes of sterility caused by flower organ development of C. vietnamensis in Hainan were analyzed in developmental biology, reproductive genetics and cell anatomy. There was no significant difference in the external morphology between the sterile flower and the normal flower, but the ovary of the sterile plant fell off after flowering, indicating that there was no normal insemination, the pollen grain did not germinate and the pollen tube did not elongate after stigma pollination. From the paraffin sections of pistil and stamen, it could be seen that the flower organ of the male sterile plant was abnormal, the tapetum of the anther was abnormally degraded and could not form mature pollen grains, but the sterile plant failed to form a mature embryo sac in the process of ovule development, resulting in hollow ovules.

Key words: Camellia vietnamensis, sterile plant, floral organ, pollen in situ germination, anatomical observation

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