Inhibitory Activity of Extracts of Antidesma montanum on the Proliferation of Breast Cancer Cells MDA-MB-231

doi:10.3969/j.issn.1000-2561.2020.08.025

Abstract

Abstract:

In order to understand the health function and active components of tropical wild plants in Hainan Island the ethanol extract of the fruits and leaves of Antidesma montanum inhibiting the proliferation of breast cancer cells MDA-MB-231 by the CCK-8 method was studied in this research. The IC₅₀ were 483 μg/mL and 306 μg/mL after 72 h treatment. The ethanol extract of the leaves of A. montanum was separated by petroleum ether, ethyl acetate, n-butanol and water respect. The ethyl acetate phase showed higher inhibitory activity than other phases, and the inhibition proliferation IC₅₀ was 150.5 μg/mL after 72 h treatment. Through the wound healing and transwell test, the ethyl acetate phase showed inhibition in migration and invasion activity of MDA-MB-231 cells. The active components could make the S phase in cell division delayed by flow cytometry detection. One of the components was identified as amentoflavone analyzed by UPLC-MS/MS. The IC₅₀of amentoflavone inhibition proliferation of MDA-MB-231 cells was 192.6 μg/mL after 72 h treatment, which showed it’s one of the active ingredients in the leaves of A.montanum.

Key words: natural products, active ingredients, breast cancer, Antidesma montanum

CLC Number:

R284.2

MA Funing,HUANG Dongmei,SONG Shun,WU Bin,GUO Gang,WANG Daxin,TAN Lin. Inhibitory Activity of Extracts of Antidesma montanum on the Proliferation of Breast Cancer Cells MDA-MB-231[J]. Chinese Journal of Tropical Crops, 2020, 41(8): 1693-1699.

Figures/Tables 7

Fig. 1 Inhibitory activity of fruits and leaves extract of A. montanum in proliferation of MDA-MB-231 cell ** means extremely significant difference (P<0.01). A: Ethanol extracts of fruits; B: Ethanol extracts of leaves; C: Different extracts phase of leaves (200 μg?mL-1); D: Ethyl acetate phase of leaves.

Fig. 2 Results of wound healing test A: Control 0 h; B: Ethyl acetate phase of leaves 0 h; C: Control 24 h; D: Ethyl acetate phase of leaves 24 h (100 μg?mL-1).

Fig. 3 Results of transwell test **means extremely significant difference (P<0.01). A: Control; B: Ethyl acetate phase of leaves (25 μg?mL-1); C: Ethyl acetate phase of leaves (50 μg?mL-1); D: Ethyl acetate phase of leaves (100 μg?mL-1).

Fig. 4 Effect of ethyl acetate phase extract of A. montanum on cell cycle of MDA-MB-231 ** means significant difference (P<0.05). A: Control; B: Ethyl acetate phase of leaves (50 μg?mL-1)。

Fig. 5 UPLC total ion chromatogram of ethyl acetate phase extract of A. montanum

Fig. 6 Mass spectrometry of amentoflavone

Fig. 7 Inhibition proliferation of MDA-MB-231 cells by amentoflavone

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