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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (8): 1693-1699.DOI: 10.3969/j.issn.1000-2561.2020.08.025

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Inhibitory Activity of Extracts of Antidesma montanum on the Proliferation of Breast Cancer Cells MDA-MB-231

MA Funing,HUANG Dongmei,SONG Shun,WU Bin,GUO Gang,WANG Daxin(),TAN Lin()   

  1. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory of Banana Genetic Improvement, Haikou, Hainan 571101, China
  • Received:2019-10-15 Revised:2019-11-29 Online:2020-08-25 Published:2020-09-17
  • Contact: WANG Daxin,TAN Lin


In order to understand the health function and active components of tropical wild plants in Hainan Island the ethanol extract of the fruits and leaves of Antidesma montanum inhibiting the proliferation of breast cancer cells MDA-MB-231 by the CCK-8 method was studied in this research. The IC50 were 483 μg/mL and 306 μg/mL after 72 h treatment. The ethanol extract of the leaves of A. montanum was separated by petroleum ether, ethyl acetate, n-butanol and water respect. The ethyl acetate phase showed higher inhibitory activity than other phases, and the inhibition proliferation IC50 was 150.5 μg/mL after 72 h treatment. Through the wound healing and transwell test, the ethyl acetate phase showed inhibition in migration and invasion activity of MDA-MB-231 cells. The active components could make the S phase in cell division delayed by flow cytometry detection. One of the components was identified as amentoflavone analyzed by UPLC-MS/MS. The IC50 of amentoflavone inhibition proliferation of MDA-MB-231 cells was 192.6 μg/mL after 72 h treatment, which showed it’s one of the active ingredients in the leaves of A.montanum.

Key words: natural products, active ingredients, breast cancer, Antidesma montanum

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