Processing Parameters of Extracting Pepper Oleoresin by Three Phase Partitioning

doi:10.3969/j.issn.1000-2561.2020.08.023

Abstract

Abstract:

In this study, three phase partitioning (TPP) was employed to extract the pepper oleoresin from black pepper powder. The effects of the addition amount of pepper powder, ammonium sulphate concentration, ratio of crude extract to t-butanol, pH and temperature on the yield of pepper oleoresin and piperine content were studied. In addition, the extraction process of pepper oleoresin was optimized by a response surface test. The optimal condition obtained was as follow: the addition amount of pepper powder 5%, ammonium sulphate concentration 10%, ratio of crude extract to t-butanol 1∶0.5, pH 4 and temperature 20℃. Under the optimal conditions, the yield of black pepper oleoresin and piperine content was 12.90% and 30.46%, respectively. The results provide a reference for the industrial extraction of pepper oleoresin.

Key words: pepper oleoresin, three phase partitioning, piperine, extraction

CLC Number:

S573
TS205

CHEN Xingxing,SONG Li,GU Fenglin,WU Guiping,TAO Rui. Processing Parameters of Extracting Pepper Oleoresin by Three Phase Partitioning[J]. Chinese Journal of Tropical Crops, 2020, 41(8): 1674-1682.

Figures/Tables 10

References 31

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因素 Factors	编码水平Levels
因素 Factors	-1	0	1
（A）pH	4	5	6
（B）提取液与叔丁醇体积比	1∶0.5	1∶1	1∶1.5
（C）温度/℃	20	40	60

因素 Factors	编码水平Levels
因素 Factors	-1	0	1
（A）pH	4	5	6
（B）提取液与叔丁醇体积比	1∶0.5	1∶1	1∶1.5
（C）温度/℃	20	40	60

编号 No.	（A）pH	（B）提取液与叔丁醇体积比 Ratio of extract to t-butanol	（C）温度 Temperature	胡椒油树脂得率 Yield of pepper oleoresin/%	胡椒碱含量 Piperine content/%
1	0	0	0	16.12	25.52
2	0	1	-1	10.05	32.81
3	0	0	0	16.54	22.83
4	0	-1	-1	13.36	30.53
5	0	-1	1	10.81	33.29
6	0	0	0	16.38	23.42
7	-1	0	1	14.64	22.48
8	0	1	1	13.62	30.12
9	-1	0	-1	16.19	24.64
10	1	1	0	11.09	33.73
11	1	-1	0	11.74	32.37
12	-1	-1	0	12.07	32.85
13	1	0	-1	13.73	22.40
14	-1	1	0	13.26	31.30
15	0	0	0	16.42	25.35
16	0	0	0	16.72	23.43
17	1	0	1	15.43	27.38

编号 No.	（A）pH	（B）提取液与叔丁醇体积比 Ratio of extract to t-butanol	（C）温度 Temperature	胡椒油树脂得率 Yield of pepper oleoresin/%	胡椒碱含量 Piperine content/%
1	0	0	0	16.12	25.52
2	0	1	-1	10.05	32.81
3	0	0	0	16.54	22.83
4	0	-1	-1	13.36	30.53
5	0	-1	1	10.81	33.29
6	0	0	0	16.38	23.42
7	-1	0	1	14.64	22.48
8	0	1	1	13.62	30.12
9	-1	0	-1	16.19	24.64
10	1	1	0	11.09	33.73
11	1	-1	0	11.74	32.37
12	-1	-1	0	12.07	32.85
13	1	0	-1	13.73	22.40
14	-1	1	0	13.26	31.30
15	0	0	0	16.42	25.35
16	0	0	0	16.72	23.43
17	1	0	1	15.43	27.38

来源Source	胡椒油树脂得率Yield of pepper oleoresin/%			胡椒碱含量Piperine content/%
来源Source	F值 F value	P值 P value	显著性 Significance	F值 F value	P值 P value	显著性 Significance
模型	123.16	< 0.0001	**	33.29	< 0.0001	**
A	29.80	< 0.0009	**	2.22	0.1796
B	2.74×10^-3	0.9597		0.15	0.7083
C	2.35	0.1695		0.77	0.4083
AB	11.60	0.0113	*	2.15	0.1860
AC	36.20	0.0005	**	14.65	0.0065	**
BC	128.36	< 0.0001	**	7.59	0.0283	*
A²	26.63	0.0013	**	1.29	0.2933
B²	797.34	< 0.0001	**	268.88	< 0.0001	**
C²	33.27	0.0007	**	0.45	0.5217
失拟项	2.16	0.2350		0.16	0.9165