Tissue Culture Technology for Pedicel Shoots of <i>Dendrobium kingianum </i>Bidwill
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (8): 1529-1534.DOI: 10.3969/j.issn.1000-2561.2020.08.004

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Tissue Culture Technology for Pedicel Shoots of Dendrobium kingianum Bidwill

CHEN Heming1,2,JIANG Nan3,LYU Fubing1,2,XIAO Wenfang1,2,LI Zuo1,2,ZHU Genfa1,2,*()   

  1. 1. Floricultural Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China
    2. Guangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Guangzhou, Guangdong 510640, China
    3. Dongguan Agricultural Science Research Center, Dongguan, Guangdong 523000, China
  • Received:2019-11-02 Revised:2020-02-28 Online:2020-08-25 Published:2020-09-17
  • Contact: ZHU Genfa

Abstract:

The induction and proliferation and rooting tests of Dendrobium kingianum Bidwill were studied using pedicel shoots as the explant. The two induction media of MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+10% CM and MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+AC 1.0 g/L+10% CM could induce buds. 70.6% pedicels died without lateral buds induced or lateral buds too weak, but it provided an effective way for seedling production and germplasm resources protection. In the proliferation culture, the medium of MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM was beneficial to proliferation culture. In the rooting and growth culture, the medium of 1/2 MS+NAA 0.3-0.5 mg/L+10% CM was suitable for the rooting culture of D. kingianum ‘Jinsika’ (DJ).

Key words: Dendrobium kingianum Bidwill, pedicel, tissue culture, induction, proliferation, rooting

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