Tissue Culture Technology for Pedicel Shoots of Dendrobium kingianum Bidwill

doi:10.3969/j.issn.1000-2561.2020.08.004

Abstract

Abstract:

The induction and proliferation and rooting tests of Dendrobium kingianum Bidwill were studied using pedicel shoots as the explant. The two induction media of MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+10% CM and MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+AC 1.0 g/L+10% CM could induce buds. 70.6% pedicels died without lateral buds induced or lateral buds too weak, but it provided an effective way for seedling production and germplasm resources protection. In the proliferation culture, the medium of MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM was beneficial to proliferation culture. In the rooting and growth culture, the medium of 1/2 MS+NAA 0.3-0.5 mg/L+10% CM was suitable for the rooting culture of D. kingianum ‘Jinsika’ (DJ).

Key words: Dendrobium kingianum Bidwill, pedicel, tissue culture, induction, proliferation, rooting

CLC Number:

S682.31

CHEN Heming,JIANG Nan,LYU Fubing,XIAO Wenfang,LI Zuo,ZHU Genfa. Tissue Culture Technology for Pedicel Shoots of Dendrobium kingianum Bidwill[J]. Chinese Journal of Tropical Crops, 2020, 41(8): 1529-1534.

Figures/Tables 14

References 30

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品种 Varieties	培养基 Culture medium	配方 Formulation	接种数 Number of inoculation	成功数 Number of success	诱导出芽率 Induction rate/%
澳0	1号诱导培养基	MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+10% CM	10	3	30.0
澳4			2	0	0.00
澳5			2	0	0.00
澳11			2	0	0.00
澳12			2	0	0.00
澳13			3	0	0.00
金斯卡			50	18	36.0
澳0	2号诱导培养基	MS+BA 2.0 mg/L+ NAA 0.5 mg/L+AC 1.0 g/L+10% CM	10	0	0.00
澳4			2	1	50.0
澳5			2	0	0.00
澳11			4	0	0.00
澳12			1	0	0.00
澳13			3	0	0.00
金斯卡			50	20	40.0

品种 Varieties	培养基 Culture medium	配方 Formulation	接种数 Number of inoculation	成功数 Number of success	诱导出芽率 Induction rate/%
澳0	1号诱导培养基	MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+10% CM	10	3	30.0
澳4			2	0	0.00
澳5			2	0	0.00
澳11			2	0	0.00
澳12			2	0	0.00
澳13			3	0	0.00
金斯卡			50	18	36.0
澳0	2号诱导培养基	MS+BA 2.0 mg/L+ NAA 0.5 mg/L+AC 1.0 g/L+10% CM	10	0	0.00
澳4			2	1	50.0
澳5			2	0	0.00
澳11			4	0	0.00
澳12			1	0	0.00
澳13			3	0	0.00
金斯卡			50	20	40.0

品种 Varieties	培养基 Culture medium	培养基配方 Medium formulation	增殖倍数 Proliferation multiple
澳0	2号增殖培养基	MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM	3.0^a
澳4	2号增殖培养基	MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM	3.0^a

品种 Varieties	培养基 Culture medium	培养基配方 Medium formulation	增殖倍数 Proliferation multiple
澳0	2号增殖培养基	MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM	3.0^a
澳4	2号增殖培养基	MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM	3.0^a

培养基 Culture medium	培养基配方 Medium formulation	增殖倍数 Proliferation multiple
1号增殖培养基	MS+6-BA 2.0 mg/L+AD 2.0 mg/L+10% CM	2.1^b
2号增殖培养基	MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM	3.2^a
3号增殖培养基	MS+6-BA 4.0 mg/L+AD 4.0 mg/L+10% CM	3.3^a