Transcriptome Analysis of Primary Stem of Chlorophyll Deficient Mutants from <i>Artocarpus heterophyllus</i>
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Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (8): 1513-1221.DOI: 10.3969/j.issn.1000-2561.2020.08.002

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Transcriptome Analysis of Primary Stem of Chlorophyll Deficient Mutants from Artocarpus heterophyllus

ZHENG Liting1,YU Xudong1,CAI Zeping1,*(),LUO Jiajia2,3,WU Fanhua4,DONG Junna1,CAO Peina1   

  1. 1. College of Forestry, Hainan University / Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, Haikou, Hainan 570228, China
    2. College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    3. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Danzhou, Hainan 571737, China
    4. College of Life Science and Pharmacy, Hainan University, Haikou, Hainan 570228, China
  • Received:2019-11-02 Revised:2020-02-28 Online:2020-08-25 Published:2020-09-17
  • Contact: CAI Zeping

Abstract:

To explore the influence of chlorophyll synthesis deficiency on the primary stem development of Artocarpus heterophyllus, the transcriptome of the primary stem of a chlorophyll deficient mutant (CDM) of A. heterophyllus was analyzed. After assembly by de novo, 295 869 Unigenes were obtained. NR, NT, Swissprot, KEGG, KOG, Pfam and GO databases were used for sequence alignment. A total of 174 291 Unigenes were annotated. After filtering the low abundance genes, 22 988 differentially expressed genes were selected. Compared with the control check (CK), there were 379 genes up-regulated in CDM and 22 609 genes down-regulated. GO classification results showed that 3712 genes were annotated and classified into three categories: molecular function, cellular component and biological process, with a total of 48 functional groups. In addition, 2080 Unigenes were involved in 19 KEGG pathways, 30 of which were involved in flavonoids biosynthesis pathways. Through transcriptome sequencing, this paper analyzed the effect of chlorophyll synthesis deficiency on the primary stem development of A. heterophyllus, which would provide data foundation for the study on the stem development of woody plants.

Key words: Artocarpus heterophyllus, chlorophyll deficient mutant, primary stem, transcriptome analysis

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