Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (7): 1450-1458.DOI: 10.3969/j.issn.1000-2561.2020.07.023

• Post-harvest Treatment & Quality Safety • Previous Articles     Next Articles

Extraction and Antioxidant Activity of Polyphenols from Gynura formosana Kitam

MA Jingfan1,2,LIN Zhemin1,LIU Ying1,LIU Ximing3,CHEN Xuemei1,2   

  1. 1. College of Life Sciences, Longyan University, Longyan, Fujian 364012, China
    2. Fujian Key Laboratory of Preventive Veterinary Medicine and Biotechnology (Longyan University), Longyan, Fujian 364012, China
    3. College of Communication and Design, Longyan University, Longyan, Fujian 364012, China
  • Received:2019-08-05 Revised:2019-10-15 Online:2020-07-25 Published:2020-08-24

Abstract:

The extracting technique of polyphenols from Gynura formosana Kitam by supercritical CO2 was optimized and the antioxidant activity in vitro and in vivo was studied to provide a theoretical basis for the development and comprehensive utilization of G. formosana Kitam. Extraction pressure, extraction time, extraction temperature, CO2 flow rate, and the consolvent concentration as single factors, the response surface method was used to optimize the extract conditions based on extraction yield. The antioxidant activity in vitro was established by DPPH and ABTS radicals scavenging rate. The antioxidant activity in vivo was evaluated using the D-galactose aging mice model. Three different doses (150, 300, 600 mg/kg every day) of polyphenols from G. formosana Kitam were given for 30 days. superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and malondialdehyde (MDA) content were determined in mice serum and liver. The best conditions for extracting polyphenols from G. formosana Kitam were extraction pressure 35 MPa, extraction time 2 h, extraction temperature 40 ℃ and CO2 flow rate 20 L/h. The yield under the optimal conditions was 5.32% DPPH and ABTS radicals scavenging rate were improved with the increasing concentration of extraction. Compared to the control group, SOD, GSH-Px and CAT activities were decreased significantly and MDA content was increased significantly in mice serum and liver in the aging model group. Compared to the aging model group, SOD, GSH-Px and CAT activities were increased significantly and MDA content was decreased significantly in mice serum and liver in extract treatment group.

Key words: Gynura formosana Kitam, supercritical CO2, radicals scavenging, antioxidant enzymes, malondialdehyde (MDA)

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