Extraction and Antioxidant Activity of Polyphenols from Gynura formosana Kitam

doi:10.3969/j.issn.1000-2561.2020.07.023

Abstract

Abstract:

The extracting technique of polyphenols from Gynura formosana Kitam by supercritical CO₂ was optimized and the antioxidant activity in vitro and in vivo was studied to provide a theoretical basis for the development and comprehensive utilization of G. formosana Kitam. Extraction pressure, extraction time, extraction temperature, CO₂flow rate, and the consolvent concentration as single factors, the response surface method was used to optimize the extract conditions based on extraction yield. The antioxidant activity in vitro was established by DPPH and ABTS radicals scavenging rate. The antioxidant activity in vivo was evaluated using the D-galactose aging mice model. Three different doses (150, 300, 600 mg/kg every day) of polyphenols from G. formosana Kitam were given for 30 days. superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and malondialdehyde (MDA) content were determined in mice serum and liver. The best conditions for extracting polyphenols from G. formosana Kitam were extraction pressure 35 MPa, extraction time 2 h, extraction temperature 40 ℃ and CO₂ flow rate 20 L/h. The yield under the optimal conditions was 5.32% DPPH and ABTS radicals scavenging rate were improved with the increasing concentration of extraction. Compared to the control group, SOD, GSH-Px and CAT activities were decreased significantly and MDA content was increased significantly in mice serum and liver in the aging model group. Compared to the aging model group, SOD, GSH-Px and CAT activities were increased significantly and MDA content was decreased significantly in mice serum and liver in extract treatment group.

Key words: Gynura formosana Kitam, supercritical CO₂, radicals scavenging, antioxidant enzymes, malondialdehyde (MDA)

CLC Number:

S647
R284.2

MA Jingfan,LIN Zhemin,LIU Ying,LIU Ximing,CHEN Xuemei. Extraction and Antioxidant Activity of Polyphenols from Gynura formosana Kitam[J]. Chinese Journal of Tropical Crops, 2020, 41(7): 1450-1458.

Figures/Tables 12

References 24

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编号 No.	X₁压力 Pressure /MPa	X₂时间 Time/h	X₃温度 Temperature/℃	X₄CO₂流量 CO₂flow rate/(L·h^-1)	提取得率 Yield/%
1	-1(30)	1(2.5)	0(40)	0(20)	4.64
2	1(40)	1	0	0	4.65
3	0(35)	0(2.0)	1(45)	1(25)	4.69
4	0	1	1	0	4.83
5	0	0	0	0	5.31
6	0	0	0	0	5.31
7	-1	0	1	0	4.71
8	-1	0	-1(35)	0	4.53
9	0	-1(1.5)	-1	0	4.56
10	0	0	-1	1	4.49
11	1	0	0	1	4.50
12	0	-1	0	1	4.27
13	0	0	1	-1(15)	4.36
14	1	0	1	0	4.74
15	0	0	0	0	5.31
16	0	1	0	1	4.65
17	0	0	-1	-1	4.50
18	1	0	0	-1	4.37
19	0	1	-1	0	4.78
20	0	-1	0	-1	4.24
21	-1	0	0	1	4.44
22	1	-1	0	0	4.49
23	0	-1	1	0	4.58
24	0	0	0	0	5.30
25	-1	0	0	-1	4.25
26	0	0	0	0	5.29
27	1	0	-1	0	4.75
28	-1	-1	0	0	4.31
29	0	1	0	-1	4.34

编号 No.	X₁压力 Pressure /MPa	X₂时间 Time/h	X₃温度 Temperature/℃	X₄CO₂流量 CO₂flow rate/(L·h^-1)	提取得率 Yield/%
1	-1(30)	1(2.5)	0(40)	0(20)	4.64
2	1(40)	1	0	0	4.65
3	0(35)	0(2.0)	1(45)	1(25)	4.69
4	0	1	1	0	4.83
5	0	0	0	0	5.31
6	0	0	0	0	5.31
7	-1	0	1	0	4.71
8	-1	0	-1(35)	0	4.53
9	0	-1(1.5)	-1	0	4.56
10	0	0	-1	1	4.49
11	1	0	0	1	4.50
12	0	-1	0	1	4.27
13	0	0	1	-1(15)	4.36
14	1	0	1	0	4.74
15	0	0	0	0	5.31
16	0	1	0	1	4.65
17	0	0	-1	-1	4.50
18	1	0	0	-1	4.37
19	0	1	-1	0	4.78
20	0	-1	0	-1	4.24
21	-1	0	0	1	4.44
22	1	-1	0	0	4.49
23	0	-1	1	0	4.58
24	0	0	0	0	5.30
25	-1	0	0	-1	4.25
26	0	0	0	0	5.29
27	1	0	-1	0	4.75
28	-1	-1	0	0	4.31
29	0	1	0	-1	4.34

来源Source	平方和SS	自由度df	均方MS	F值F value	P值P value	显著性Significance
模型	3.23	14	0.23	575.37	< 0.0001	**
X₁	0.032	1	0.032	79.82	< 0.0001	**
X₂	0.17	1	0.17	434.57	< 0.0001	**
X₃	6.557×10^-3	1	6.557×10^-3	16.33	0.0012	*
X₄	0.082	1	0.082	203.12	< 0.0001	**
X₁ X₂	6.593×10^-3	1	6.593×10^-3	16.43	0.0012	*
X₁ X₃	9.209×10^-3	1	9.209×10^-3	22.94	0.0003	*
X₁ X₄	8.718×10^-3	1	8.718×10^-3	2.17	0.1627
X₂ X₃	2.180×10^-3	1	2.180×10^-3	0.54	0.4734
X₂ X₄	0.020	1	0.020	49.00	< 0.0001	**
X₃ X₄	0.031	1	0.031	78.19	< 0.0001	**
X₁²	0.95	1	0.95	2364.16	< 0.0001	**
X₂²	0.99	1	0.99	2456.29	< 0.0001	**
X₃²	0.37	1	0.37	933.87	< 0.0001	**
X₄²	1.91	1	1.91	4765.41	< 0.0001	**
残差	5.620×10^-3	14	4.014×10^-4
失拟项	4.922×10^-3	10	4.922×10^-4	2.82	0.1646
纯误差	6.974×10^-4	4	1.744×10^-4
总和	3.24	28

来源Source	平方和SS	自由度df	均方MS	F值F value	P值P value	显著性Significance
模型	3.23	14	0.23	575.37	< 0.0001	**
X₁	0.032	1	0.032	79.82	< 0.0001	**
X₂	0.17	1	0.17	434.57	< 0.0001	**
X₃	6.557×10^-3	1	6.557×10^-3	16.33	0.0012	*
X₄	0.082	1	0.082	203.12	< 0.0001	**
X₁ X₂	6.593×10^-3	1	6.593×10^-3	16.43	0.0012	*
X₁ X₃	9.209×10^-3	1	9.209×10^-3	22.94	0.0003	*
X₁ X₄	8.718×10^-3	1	8.718×10^-3	2.17	0.1627
X₂ X₃	2.180×10^-3	1	2.180×10^-3	0.54	0.4734
X₂ X₄	0.020	1	0.020	49.00	< 0.0001	**
X₃ X₄	0.031	1	0.031	78.19	< 0.0001	**
X₁²	0.95	1	0.95	2364.16	< 0.0001	**
X₂²	0.99	1	0.99	2456.29	< 0.0001	**
X₃²	0.37	1	0.37	933.87	< 0.0001	**
X₄²	1.91	1	1.91	4765.41	< 0.0001	**
残差	5.620×10^-3	14	4.014×10^-4
失拟项	4.922×10^-3	10	4.922×10^-4	2.82	0.1646
纯误差	6.974×10^-4	4	1.744×10^-4
总和	3.24	28

组别 Group	SOD活性 SOD activity /(U?mg^-1)	GSH-Px活性 GSH-Px activity /(U?mg^-1)	CAT活性 CAT activity /(U?mg^-1)	MDA含量 MDA content /(μmol?g^-1)
空白组	207.3±14.11	643.7±47.46	11.2±1.93	7.5±0.68
模型组	98.5±7.32^**	567.3±52.11^*	8.4±0.48^**	9.2±0.94^*
低剂量组（150 mg/kg）	134.7±8.47^##	644.0±39.43^#	8.5±0.98	7.5±0.57^#
中剂量组（300 mg/kg）	141.8±5.24^##	657.2±54.23^##	9.7±0.95^#	6.9±0.38^##
高剂量组（600 mg/kg）	149.8±9.43^##	707.7±62.37^##	11.4±0.97^##	6.2±0.66^##