Genetic Analysis of Resistance of Watermelon Cultivars ‘Heipi’ to Aphis gossypii

doi:10.3969/j.issn.1000-2561.2020.07.018

Abstract

Abstract:

To get stable resistant watermelon cultivars, the genetic analysis of the resistance of watermelon cultivar ‘Heipi’ to Aphis gossypii was proceeded by the ratio of aphid number at seedling in a greenhouse added with RAPD and SCAR. The reciprocal-hybrid F₁ of ‘Heipi’ and ‘Hualv’ was resistant while the resistant and susceptible plants in F₂ hybrid fitted the separation ratio of 3:1 by χ ²test. Four resistant cultivars, four susceptible cultivars, ten resistant F₂single plants and ten susceptible F₂single plants were verified with screened RAPD WO4₆₀₀and SCAR WO4-S₅₃₀. The results indicated that the resistance of the parent ‘Heipi’ to A. gossypii was controlled by a dominant single gene which was stably inherited, ‘Heipi’, ‘Lvmeiren’, ‘Heimeiren’ and ‘Huilan’ were aphid-resistant cultivars, ‘Hualv’, ‘Fengguang’, ‘Huibao’ and ‘Tianmeiren’ were aphid-susceptible cultivars.

Key words: watermelon cultivars, Aphis gossypii, aphid-resistance, genetic analysis

CLC Number:

S436.5

CHEN Qing,LIANG Xiao,WU Chunling,LIU Ying,XU Xuelian. Genetic Analysis of Resistance of Watermelon Cultivars ‘Heipi’ to Aphis gossypii[J]. Chinese Journal of Tropical Crops, 2020, 41(7): 1415-1419.

Figures/Tables 7

Tab. 1 Genetic analysis of watermelon resistance to A. gossypii

杂交组合 Hybridization combination	F₁			F₂			分离比 Separation ratio	χ²_0.05
杂交组合 Hybridization combination	植株总数 No. of plants	平均单株蚜量比值 Average aphid ratio per plant	表型 phenotype	植株总数 No. of plants	抗 Resistant	感 Susceptible	分离比 Separation ratio	χ²_0.05
‘黑皮’×‘花绿’	97	0.22	HR	625	468	157	2.98:1	0.0005
‘花绿’×‘黑皮’	101	0.24	HR	598	456	142	3.21:1	0.437

Fig. 1 RAPD primers to amplify by PCR in mixed DNA of F2 resistant DNA and susceptible DNA 1-2: Primer WO4; 3-4: Primer WO9; 5-6: PrimerWO18; 7-8: Primer WO27; 9-10: Primer WO39; M: DNA marker.

Fig. 2 Primer WO4 to amplify by PCR in resistant parent DNA, susceptible parent DNA, F1 DNA, mixed DNA of F2 resistant DNA and susceptible DNA, F2 single resistant plant DNA, F2 single susceptible plant DNA 1: F2 susceptible DNA; 2: Susceptible parent ‘Hualv’; 3: F2 single susceptible plant; 4: F2 resistant DNA; 5: Resistant parent ‘Heipi’; 6: F1; 7: F2 single resistant plant; M: DNA marker.

Fig. 3 Primer WO4 to amplify by PCR in 20 DNA of F2 single resistant and susceptible plants 1-10: F2 aphid-susceptible plant; 11-20: F2 aphid-resistant plant; M: DNA marker.

Fig. 4 Primer WO4 to amplify by PCR in 8 DNA of resistant and susceptible cultivars 1-4: Susceptible cultivars ‘Hualv’, ‘Fengguang’, ‘Huibao’ and ‘Tianmeiren’; 5-8: Resistant cultivars ‘Heipi’, ‘Lvmeiren’, ‘Heimeiren’ and ‘Huilan’; M: DNA marker.

Fig. 5 Primer WO4-S to amplify by PCR in 8 DNA of resistant and susceptible cultivars 1-4: Susceptible cultivars; 5-8: Resistant cultivars; M: DNA marker.

Fig. 6 Primer WO4 to amplify by PCR in DNA of F2 single resistant and susceptible plants 1-10: F2 aphid- susceptible plant; 11-20: F2 aphid-resistant plant; M: DNA marker.

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