Bioinformatics Analysis of MYB Transcription Factor Family in Blumea balsamifera

doi:10.3969/j.issn.1000-2561.2020.07.004

Abstract

Abstract:

MYB is one of the largest transcription factors family and has a wide range of biological functions in plants. Based on the full-length transcriptome database of Blumea balsamifera, DNAMAN 6.0, MEGA 5.05 software and ProtParam, WebLogo and SOPMA online sites were used to analyze and predict the types, physicochemical properties, advanced structure of amino acid sequence, conserved domain and phylogenetic evolution of MYB transcription factor in B. balsamifera, The results showed that 127 BbMYB genes were extracted, and 47 protein sequences with MYB conserved domains were predicted. According to the domain, they could be divided into 1R-BbMYB and R2R3-MYB. Meanwhile, the R2-BbMYB and R3-BbMYB motifs contained three conserved tryptophans, while R1-BbMYB contained only two. Morever, all proteins of MYB family in B. balsamifera were hydrophilic with high thermal stability and rich in basic amino acids. And most of the proteins were irregular curls except the R2R3-BbMYB3, R2R3-BbMYB4 and 1R-BbMYB15. The MYB family of B. balsamifera evolved into two major categories and six subcategories by comparing with the evolutionary tree of Arabidopsis thaliana. It would provide a method for further research on the functional identification of the MYB family in B. balsamifera by the adjacent or evolutionary relationship with the same function.

Key words: Blumea balsamifera, MYB transcription factor, bioinformatics

CLC Number:

GUAN Lingliang,ZHA Ying,BAI Lin,YU Fulai,CHEN Zhenxia,CHEN Xiaolu,XIE Xiaoli,WANG Kai,HUANG Mei. Bioinformatics Analysis of MYB Transcription Factor Family in Blumea balsamifera[J]. Chinese Journal of Tropical Crops, 2020, 41(7): 1305-1312.

Figures/Tables 5

References 25

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基因编号 Gene number	类型 Type	氨基酸残基数 Number of amino acid/aa	分子量 Molecular weight/Da	脂肪系数 Aliphatic index	等电点 Isoelectric point	总平均亲水性 GRAVY
BbMYB1	2R-MYB	294	31 982.39	60.41	6.26	-0.718
BbMYB3	2R-MYB	804	92 171.87	73.25	6.72	-0.935
BbMYB4	2R-MYB	158	18 353.65	57.41	9.16	-1.089
BbMYB6	2R-MYB	305	34 021.06	56.89	8.34	-0.704
BbMYB7	2R-MYB	368	41 595.82	55.76	6.13	-0.908
BbMYB8	2R-MYB	395	43 216.10	71.37	9.26	-0.459
BbMYB9	2R-MYB	303	33 920.11	66.01	7.61	-0.712
BbMYB10	2R-MYB	219	25 298.57	53.47	9.53	-1.016
BbMYB11	2R-MYB	546	60 109.08	64.29	5.14	-0.691
BbMYB12	2R-MYB	188	21 278.31	70.53	9.47	-0.820
BbMYB13	2R-MYB	239	27 447.15	69.71	8.93	-0.714
BbMYB14	2R-MYB	457	53 107.84	84.27	8.73	-0.653
BbMYB16	2R-MYB	357	41 968.78	74.31	8.23	-0.796
BbMYB17	2R-MYB	827	92 670.50	66.37	5.09	-0.748
BbMYB47	2R-MYB	1307	142 984.70	65.36	5.74	-0.662
BbMYB2	1R-MYB	503	56 870.07	69.58	6.05	-0.674
BbMYB5	1R-MYB	222	25 181.41	73.83	6.24	-0.801
BbMYB15	1R-MYB	298	34 950.43	83.46	9.20	-0.573
BbMYB18	1R-MYB	662	73 575.78	56.87	9.20	-1.226
BbMYB19	1R-MYB	615	68 143.18	69.72	5.70	-0.694
BbMYB20	1R-MYB	400	44 308.55	59.15	8.47	-0.831
BbMYB21	1R-MYB	799	88 442.20	64.12	6.25	-0.660
BbMYB22	1R-MYB	146	16 815.87	52.67	5.52	-0.855
BbMYB23	1R-MYB	373	41 411.59	60.29	9.27	-0.832
BbMYB24	1R-MYB	448	52 263.28	64.60	9.38	-1.028
BbMYB25	1R-MYB	327	35 118.95	66.48	5.87	-0.516
BbMYB26	1R-MYB	259	28 817.59	69.23	7.11	-0.607
BbMYB27	1R-MYB	296	33 140.47	68.48	8.78	-0.646
BbMYB28	1R-MYB	167	18 967.51	70.06	10.19	-0.634
基因编号 Gene number	类型 Type	氨基酸残基数 Number of amino acid/aa	分子量 Molecular weight/Da	脂肪系数 Aliphatic index	等电点 Isoelectric point	总平均亲水性 GRAVY
BbMYB29	1R-MYB	449	50 334.14	76.28	9.30	-0.695
BbMYB30	1R-MYB	200	22 312.07	70.70	9.85	-0.608
BbMYB31	1R-MYB	467	52 388.68	78.97	9.25	-0.622
BbMYB32	1R-MYB	143	15 947.70	62.80	10.20	-0.850
BbMYB33	1R-MYB	140	15 660.00	85.14	11.58	-0.509
BbMYB34	1R-MYB	210	24 681.50	45.48	7.81	-1.022
BbMYB35	1R-MYB	696	76 791.71	61.82	5.89	-0.796
BbMYB36	1R-MYB	716	78 648.78	61.62	5.59	-0.756
BbMYB37	1R-MYB	229	25 487.85	66.81	7.75	-0.347
BbMYB38	1R-MYB	308	34 412.54	72.82	5.40	-0.681
BbMYB39	1R-MYB	211	23 546.53	66.11	5.86	-0.547
BbMYB40	1R-MYB	625	69 380.68	71.58	5.83	-0.638
BbMYB41	1R-MYB	225	24 703.54	72.40	8.54	-0.616
BbMYB42	1R-MYB	559	61 754.45	62.33	5.82	-0.700
BbMYB43	1R-MYB	541	59 732.29	63.33	5.77	-0.693
BbMYB44	1R-MYB	406	45 500.58	77.07	5.82	-0.497
BbMYB45	1R-MYB	152	17 120.45	89.74	4.70	-0.245
BbMYB46	1R-MYB	753	83 001.81	61.43	5.63	-0.714

基因编号 Gene number	类型 Type	氨基酸残基数 Number of amino acid/aa	分子量 Molecular weight/Da	脂肪系数 Aliphatic index	等电点 Isoelectric point	总平均亲水性 GRAVY
BbMYB1	2R-MYB	294	31 982.39	60.41	6.26	-0.718
BbMYB3	2R-MYB	804	92 171.87	73.25	6.72	-0.935
BbMYB4	2R-MYB	158	18 353.65	57.41	9.16	-1.089
BbMYB6	2R-MYB	305	34 021.06	56.89	8.34	-0.704
BbMYB7	2R-MYB	368	41 595.82	55.76	6.13	-0.908
BbMYB8	2R-MYB	395	43 216.10	71.37	9.26	-0.459
BbMYB9	2R-MYB	303	33 920.11	66.01	7.61	-0.712
BbMYB10	2R-MYB	219	25 298.57	53.47	9.53	-1.016
BbMYB11	2R-MYB	546	60 109.08	64.29	5.14	-0.691
BbMYB12	2R-MYB	188	21 278.31	70.53	9.47	-0.820
BbMYB13	2R-MYB	239	27 447.15	69.71	8.93	-0.714
BbMYB14	2R-MYB	457	53 107.84	84.27	8.73	-0.653
BbMYB16	2R-MYB	357	41 968.78	74.31	8.23	-0.796
BbMYB17	2R-MYB	827	92 670.50	66.37	5.09	-0.748
BbMYB47	2R-MYB	1307	142 984.70	65.36	5.74	-0.662
BbMYB2	1R-MYB	503	56 870.07	69.58	6.05	-0.674
BbMYB5	1R-MYB	222	25 181.41	73.83	6.24	-0.801
BbMYB15	1R-MYB	298	34 950.43	83.46	9.20	-0.573
BbMYB18	1R-MYB	662	73 575.78	56.87	9.20	-1.226
BbMYB19	1R-MYB	615	68 143.18	69.72	5.70	-0.694
BbMYB20	1R-MYB	400	44 308.55	59.15	8.47	-0.831
BbMYB21	1R-MYB	799	88 442.20	64.12	6.25	-0.660
BbMYB22	1R-MYB	146	16 815.87	52.67	5.52	-0.855
BbMYB23	1R-MYB	373	41 411.59	60.29	9.27	-0.832
BbMYB24	1R-MYB	448	52 263.28	64.60	9.38	-1.028
BbMYB25	1R-MYB	327	35 118.95	66.48	5.87	-0.516
BbMYB26	1R-MYB	259	28 817.59	69.23	7.11	-0.607
BbMYB27	1R-MYB	296	33 140.47	68.48	8.78	-0.646
BbMYB28	1R-MYB	167	18 967.51	70.06	10.19	-0.634
基因编号 Gene number	类型 Type	氨基酸残基数 Number of amino acid/aa	分子量 Molecular weight/Da	脂肪系数 Aliphatic index	等电点 Isoelectric point	总平均亲水性 GRAVY
BbMYB29	1R-MYB	449	50 334.14	76.28	9.30	-0.695
BbMYB30	1R-MYB	200	22 312.07	70.70	9.85	-0.608
BbMYB31	1R-MYB	467	52 388.68	78.97	9.25	-0.622
BbMYB32	1R-MYB	143	15 947.70	62.80	10.20	-0.850
BbMYB33	1R-MYB	140	15 660.00	85.14	11.58	-0.509
BbMYB34	1R-MYB	210	24 681.50	45.48	7.81	-1.022
BbMYB35	1R-MYB	696	76 791.71	61.82	5.89	-0.796
BbMYB36	1R-MYB	716	78 648.78	61.62	5.59	-0.756
BbMYB37	1R-MYB	229	25 487.85	66.81	7.75	-0.347
BbMYB38	1R-MYB	308	34 412.54	72.82	5.40	-0.681
BbMYB39	1R-MYB	211	23 546.53	66.11	5.86	-0.547
BbMYB40	1R-MYB	625	69 380.68	71.58	5.83	-0.638
BbMYB41	1R-MYB	225	24 703.54	72.40	8.54	-0.616
BbMYB42	1R-MYB	559	61 754.45	62.33	5.82	-0.700
BbMYB43	1R-MYB	541	59 732.29	63.33	5.77	-0.693
BbMYB44	1R-MYB	406	45 500.58	77.07	5.82	-0.497
BbMYB45	1R-MYB	152	17 120.45	89.74	4.70	-0.245
BbMYB46	1R-MYB	753	83 001.81	61.43	5.63	-0.714