Ultrasound-microwave Assisted Extraction and Antioxidant Activity of Total Flavonoids from Hibiscus sabdariffa L.

doi:10.3969/j.issn.1000-2561.2020.06.024

Abstract

Abstract:

The ultrasonic-microwave assisted extraction of the flavonoids from Hibiscus sabdariffa L. was optimized by response surface analysis with a Box-Behnken design. The effects of five important factors, liquid-material ratio, ethanol concentraion, extraction time, microwave power and the extraction times, on the flavonoids extraction of H. sabdariffa Lwere investigated in the single factor experiments. The regression model of the liquid-material ratio, ethanol concentration and microwave power to solvent was established. The optimum technology condition for the ultrasonic-microwave assisted extraction of the flavonoids from H. sabdariffa L. was determined as follow: the ethanol concentration 40%, liquid-material ratio 50∶1 (mL/g), microwave power 250 W, extraction time 20 min and ultrasonic power 50 W, extracted twice. Under the conditions, the flavonoids yield was 40.2 mg/g. Using vitamin C as the control, the antioxidant activity of the crude extract and purified flavonoids was studied. The crude extract and purified flavonoids from H. sabdariffa L. had a strong antioxidant activity, and they had an antioxidation effect on DPPH and O ^-₂?, and the scavenging activity had an obvious dose-effect relationship of concentration, but the antioxidant activity was weaker than that of vitamin C.

Key words: response surface methodology, ultrasound-microwave assisted, total flavonoids from Hibiscus sabdariffa L., extraction, antioxidation activity

CLC Number:

S681
TS201.2

HUANG Qiong,HUANG Xiaomei. Ultrasound-microwave Assisted Extraction and Antioxidant Activity of Total Flavonoids from Hibiscus sabdariffa L.[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1242-1250.

Figures/Tables 15

References 23

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水平 Levels	因素Factors
水平 Levels	A液固比 Liquid-material ratio/(mL·g^-1)	B 乙醇浓度 Ethanol concentration/%	C 微波功率 Microwave power/W
-1	40:1	30	200
0	50:1	40	250
1	60:1	50	300

水平 Levels	因素Factors
水平 Levels	A液固比 Liquid-material ratio/(mL·g^-1)	B 乙醇浓度 Ethanol concentration/%	C 微波功率 Microwave power/W
-1	40:1	30	200
0	50:1	40	250
1	60:1	50	300

因素Factor	F值 F value	P值 P value
液固比/(mL·g^-1)	369.15	<0.0001^**
乙醇浓度/%	145.70	<0.0001^**
提取时间/min	9.62	0.0173^*
微波功率/W	50.8	0.0002^**
提取次数	4.19	0.08

因素Factor	F值 F value	P值 P value
液固比/(mL·g^-1)	369.15	<0.0001^**
乙醇浓度/%	145.70	<0.0001^**
提取时间/min	9.62	0.0173^*
微波功率/W	50.8	0.0002^**
提取次数	4.19	0.08

方差来源 Sources of variance	平方和 Quadratic sum	自由度 Variance	均方 Mean square	F值 F value	P值 P value	显著性 Significant
模型	1587.66	9	176.41	457.78	<0.0001	**
A	40.05	1	40.05	103.93	<0.0001	**
B	14.05	1	14.05	36.45	0.0005	**
C	3.00	1	3.00	7.79	0.0269	*
AB	82.81	1	82.81	214.89	<0.0001	**
AC	1.32	1	1.32	3.43	0.1064
BC	12.96	1	12.96	33.63	0.0007	**
A²	334.45	1	334.45	867.90	<0.0001	**
B²	610.11	1	610.11	1583.24	<0.0001	**
C²	342.00	1	342.00	887.49	<0.0001	**
残差	2.70	7	0.39
失拟项	1.90	3	0.63	3.16	0.1476
纯误差	0.80	4	0.20
所有项	1590.36	16