Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1202-1207.DOI: 10.3969/j.issn.1000-2561.2020.06.019

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Sequence Characteristics of Laccase Gene Cglac3 and Its Expression in Two Infection-Related Gene Mutants from Colletotrichum gloeosporioides on Mango

ZHONG Changkai1,XIAO Chunli1,ZHANG He2,PU Jinji2,WU Qiuyu1,LIU Yanli1,LIU Xiaomei1,*()   

  1. 1. College of Plant Protection, Hainan University / Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, Hainan 570228, China
    2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received:2019-08-01 Revised:2019-08-23 Online:2020-06-25 Published:2020-07-30
  • Contact: LIU Xiaomei

Abstract:

In this experiment, a laccase gene Cglac3 was cloned by homologously from Colletotrichum gloeosporioides, which is the fungal pathogen of mango anthracnose, and its expressions during infection process, in laccase gene Cglac1 and exoglucanase gene CgCBH1 deletion knockout mutants, were comparatively analyzed using real-time fluorescent quantitative PCR. Cglac3 was 1842 bp in size with three introns of 56, 51 and 58 bp respectively. The open reading frame of Cglac3 encoded 558 amino acids with a protein molecular weight of 61.38 kDa and an isoelectric point (PI) of 4.50. Cglac3 was highly expressed at 6 h after inoculation when the fungal spores were germing and forming appressoria, increased rapidly at 12 h after inoculation when the fugus were invading. Since then, the expresson of Cglac3 fluctuated during hyphal expansion and leaf necrosis, but both were much higher than the expression level at 0 h. It suggested that Cglac3 played an important role during C. gloeosporioides infection, might be a key virulent factor. Compared with the wild type, the expression of Cglac3 decreased by 74% in the C. gloeosporioides ΔLAC1 mutant, and decreased by 56% in the ΔCgCBH1 mutant. Interestingly, the expression of LAC1 was also decreased by 68% in the ΔCgCBH1 mutant, consistent with the trend of Cglac3 expression, indicating that LAC1 affected the expression of Cglac3, and CgCBH1 affected both the expressions of Cglac3 and LAC1.

Key words: mango, laccase, Cglac3 gene, qRT-PCR

CLC Number: