Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1179-1188.DOI: 10.3969/j.issn.1000-2561.2020.06.016

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Tissue Culture and Rapid Propagation of Dendrobium Hibiki

MO Yuanqi1,2,LI Hanwen3,JIANG Nan3,ZHENG Feng2,FANG Lin2,LI Lin2,ZHOU Yiqing2,WU Kunlin2,ZENG Songjun2,4,*()   

  1. 1. Institute of Crop Germplasm Resources, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550006, China
    2. Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China
    3. Dongguan Agricultural Science Research Center, Dongguan, Guangdong 523000, China
    4. Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China
  • Received:2019-06-18 Revised:2019-10-28 Online:2020-06-25 Published:2020-07-30
  • Contact: ZENG Songjun

Abstract:

The young pseudobulbs of Dendrobium Hibiki were taken as the explants for tissue culture and rapid propagation. The results showed that the success rate of explants disinfection reached 92.50% when treating the explants with proper disinfection methodsby 75% alcohol and 0.1% HgCl2. The modified MS supplemented with 3 mg/L 6-BA and 0.5 mg/L NAA was the most suitable medium for axillary bud induction, in which the induction rate was 91.90%. MS supplemented with 5.0 mg/L 6-BA and 0.5 mg/L NAA was the most suitable medium for the multiplication of clustered shoots, in which the multiplication coefficient was 2.77. 1/2 MS supplemented with 0.5 mg/L IBA, 1.5 g/L activated carbon, 30 g/L sucrose and 100 g/L potato homogenate was the most suitable medium for the rooting and growth, the rooting rate was 100% and growth status of the plantlets was the best. On the mixture of Zhijing stone: coconut husk=1∶1 (V∶V), the plantlets transplanted grew fastly and the survival percentage was 95.56%.

Key words: Dendrobium Hibiki, pseudobulbs, proliferation, transplantion

CLC Number: