Tissue Culture and Rapid Propagation of Dendrobium Hibiki

doi:10.3969/j.issn.1000-2561.2020.06.016

Abstract

Abstract:

The young pseudobulbs of Dendrobium Hibiki were taken as the explants for tissue culture and rapid propagation. The results showed that the success rate of explants disinfection reached 92.50% when treating the explants with proper disinfection methodsby 75% alcohol and 0.1% HgCl₂. The modified MS supplemented with 3 mg/L 6-BA and 0.5 mg/L NAA was the most suitable medium for axillary bud induction, in which the induction rate was 91.90%. MS supplemented with 5.0 mg/L 6-BA and 0.5 mg/L NAA was the most suitable medium for the multiplication of clustered shoots, in which the multiplication coefficient was 2.77. 1/2 MS supplemented with 0.5 mg/L IBA, 1.5 g/L activated carbon, 30 g/L sucrose and 100 g/L potato homogenate was the most suitable medium for the rooting and growth, the rooting rate was 100% and growth status of the plantlets was the best. On the mixture of Zhijing stone: coconut husk=1∶1 (V∶V), the plantlets transplanted grew fastly and the survival percentage was 95.56%.

Key words: Dendrobium Hibiki, pseudobulbs, proliferation, transplantion

CLC Number:

Q813.1+2

MO Yuanqi,LI Hanwen,JIANG Nan,ZHENG Feng,FANG Lin,LI Lin,ZHOU Yiqing,WU Kunlin,ZENG Songjun. Tissue Culture and Rapid Propagation of Dendrobium Hibiki[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1179-1188.

Figures/Tables 8

References 35

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基质组合 Matrix combination	移栽成活率 Survival rate of transplantation/%	生长状况 Growth status	基质组合 Matrix combination	移栽成活率 Survival rate of transplantation/%	生长状况 Growth status
植金石:松树皮1:1	88.89±2.22^abcd	15.56%叶宽增加,+++	兰石:松树皮1:1	51.11±2.22^lm	17.8%叶宽增加,+++
植金石:松树皮1:2	91.11±4.44^abc	13.33%叶宽增加,+++	兰石:松树皮1:2	48.89±2.22^m	11.11%叶宽增加,+++
植金石:松树皮1:3	82.22±2.22^cdefg	13.33%叶宽增加,++++	兰石:松树皮1:3	53.33±3.85^lm	15.56%叶宽增加,+++
植金石:椰壳1:1	95.56±4.44^ab	37.78%叶宽增加,+++++	兰石:椰壳1:1	86.67±6.68^bcde	24.45%叶宽增加,++
植金石:椰壳1:2	73.33±3.85^fghi	6.67%叶宽增加,++++	兰石:椰壳1:2	60.00±0.00^jklm	11.11%叶宽增加,++
植金石:椰壳1:3	55.56±8.01^klm	4.44%叶宽增加,++++	兰石:椰壳1:3	82.22±4.45^cdefg	4.44%叶宽增加,+
植金石:泥炭土1:1	95.55±2.22^ab	66.67%叶宽增加,+++++	兰石:泥炭土1:1	84.44±5.88^bcdef	5.56%叶宽增加,+++
植金石:泥炭土1:2	73.33±3.85^fghi	48.89%叶宽增加,++++	兰石:泥炭土1:2	66.67±3.85^hijk	40.00%叶宽增加,+++
植金石:泥炭土1:3	88.89±2.22^abcd	46.67%叶宽增加,+++	兰石:泥炭土1:3	82.22±2.22^cdefg	28.89%叶宽增加,+++
泥炭土:松树皮1:1	100.00±0.00^a	64.44%叶宽增加,++++	植金石	80.00±0.00^cdefg	46.67%叶宽增加,+++
泥炭土:松树皮1:2	84.44±4.44^bcdef	40.00%叶宽增加,+++	松树皮	62.23±2.23^ijkl	24.44%叶宽增加,++
泥炭土:松树皮1:3	75.56±4.44^efgh	37.78%叶宽增加,++++	椰壳	77.78±3.85^defgh	17.78%叶宽增加,++
泥炭土:椰壳1:1	82.22±2.22^cdefg	37.78%叶宽增加,++++	水苔	82.22±2.22^cdefg	46.67%叶宽增加,++++
泥炭土:椰壳1:2	77.78±4.45^defgh	26.67%叶宽增加,++++	泥炭土	83.34±3.34^bcdefg	53.33%叶宽增加,+++++
泥炭土:椰壳1:3	73.33±0.00^fghi	15.56%叶宽增加,++++	兰石	82.22±2.22^cdefg	53.33%叶宽增加,++

基质组合 Matrix combination	移栽成活率 Survival rate of transplantation/%	生长状况 Growth status	基质组合 Matrix combination	移栽成活率 Survival rate of transplantation/%	生长状况 Growth status
植金石:松树皮1:1	88.89±2.22^abcd	15.56%叶宽增加,+++	兰石:松树皮1:1	51.11±2.22^lm	17.8%叶宽增加,+++
植金石:松树皮1:2	91.11±4.44^abc	13.33%叶宽增加,+++	兰石:松树皮1:2	48.89±2.22^m	11.11%叶宽增加,+++
植金石:松树皮1:3	82.22±2.22^cdefg	13.33%叶宽增加,++++	兰石:松树皮1:3	53.33±3.85^lm	15.56%叶宽增加,+++
植金石:椰壳1:1	95.56±4.44^ab	37.78%叶宽增加,+++++	兰石:椰壳1:1	86.67±6.68^bcde	24.45%叶宽增加,++
植金石:椰壳1:2	73.33±3.85^fghi	6.67%叶宽增加,++++	兰石:椰壳1:2	60.00±0.00^jklm	11.11%叶宽增加,++
植金石:椰壳1:3	55.56±8.01^klm	4.44%叶宽增加,++++	兰石:椰壳1:3	82.22±4.45^cdefg	4.44%叶宽增加,+
植金石:泥炭土1:1	95.55±2.22^ab	66.67%叶宽增加,+++++	兰石:泥炭土1:1	84.44±5.88^bcdef	5.56%叶宽增加,+++
植金石:泥炭土1:2	73.33±3.85^fghi	48.89%叶宽增加,++++	兰石:泥炭土1:2	66.67±3.85^hijk	40.00%叶宽增加,+++
植金石:泥炭土1:3	88.89±2.22^abcd	46.67%叶宽增加,+++	兰石:泥炭土1:3	82.22±2.22^cdefg	28.89%叶宽增加,+++
泥炭土:松树皮1:1	100.00±0.00^a	64.44%叶宽增加,++++	植金石	80.00±0.00^cdefg	46.67%叶宽增加,+++
泥炭土:松树皮1:2	84.44±4.44^bcdef	40.00%叶宽增加,+++	松树皮	62.23±2.23^ijkl	24.44%叶宽增加,++
泥炭土:松树皮1:3	75.56±4.44^efgh	37.78%叶宽增加,++++	椰壳	77.78±3.85^defgh	17.78%叶宽增加,++
泥炭土:椰壳1:1	82.22±2.22^cdefg	37.78%叶宽增加,++++	水苔	82.22±2.22^cdefg	46.67%叶宽增加,++++
泥炭土:椰壳1:2	77.78±4.45^defgh	26.67%叶宽增加,++++	泥炭土	83.34±3.34^bcdefg	53.33%叶宽增加,+++++
泥炭土:椰壳1:3	73.33±0.00^fghi	15.56%叶宽增加,++++	兰石	82.22±2.22^cdefg	53.33%叶宽增加,++

培养基 Culture medium	腋芽的诱导率 Induction rate of axillary buds/%			腋芽起始萌动时间 Initial germination time of axillary buds/d
培养基 Culture medium	上段	中段	下段	上段	中段	下段
MS+3.0 mg/L 6-BA	0^k	65.56±2.94^fg	70.37±3.70^defg	0^j	17.67±0.33^d	16.00±0.58^ef
改良MS	0^k	71.48±3.29^defg	74.26±2.27^cdef	0^j	17.00±0.00^de	16.00±0.58^ef
改良MS+1.0mg/L 6-BA	23.33±3.33^h	78.53±0.74^bcd	85.19±3.70^ab	22.67±0.33^a	17.00±0.00^de	16.00±0.58^ef
改良MS+3.0 mg/L 6-BA	24.07±3.03^h	81.90±1.90^bc	91.90±4.23^a	22.00±0.00^ab	15.00±0.00^fg	15.00±0.00^fg
改良MS+5.0 mg/L 6-BA	16.67±3.33^ij	75.19±2.59^cde	75.47±2.49^cde	21.00±0.58^bc	13.00±0.00^h	14.00±0.58^gh
改良MS+7.0 mg/L 6-BA	10.37±0.37^j	64.45±2.22^g	68.06±3.68^efg	20.67±0.33^c	11.67±0.33ⁱ	11.83±0.31ⁱ

培养基 Culture medium	腋芽的诱导率 Induction rate of axillary buds/%			腋芽起始萌动时间 Initial germination time of axillary buds/d
培养基 Culture medium	上段	中段	下段	上段	中段	下段
MS+3.0 mg/L 6-BA	0^k	65.56±2.94^fg	70.37±3.70^defg	0^j	17.67±0.33^d	16.00±0.58^ef
改良MS	0^k	71.48±3.29^defg	74.26±2.27^cdef	0^j	17.00±0.00^de	16.00±0.58^ef
改良MS+1.0mg/L 6-BA	23.33±3.33^h	78.53±0.74^bcd	85.19±3.70^ab	22.67±0.33^a	17.00±0.00^de	16.00±0.58^ef
改良MS+3.0 mg/L 6-BA	24.07±3.03^h	81.90±1.90^bc	91.90±4.23^a	22.00±0.00^ab	15.00±0.00^fg	15.00±0.00^fg
改良MS+5.0 mg/L 6-BA	16.67±3.33^ij	75.19±2.59^cde	75.47±2.49^cde	21.00±0.58^bc	13.00±0.00^h	14.00±0.58^gh
改良MS+7.0 mg/L 6-BA	10.37±0.37^j	64.45±2.22^g	68.06±3.68^efg	20.67±0.33^c	11.67±0.33ⁱ	11.83±0.31ⁱ

植物生长调节剂Plant growth regulator/(mg·L^-1)					不定芽增殖系数 Adventitious bud proliferation coefficient	生长状况 Growth status
NAA	6-BA	TDZ	KT	2,4-D	不定芽增殖系数 Adventitious bud proliferation coefficient	生长状况 Growth status
0	0	0	0	0	0.29±0.02^fg	长势正常
0.5	0	0	0	0	1.41±0.29^cd	长势正常
0.5	0.5	0	0	0	1.80±0.33^bc	长势正常
0.5	1.0	0	0	0	1.90±0.21^bc	长势正常
0.5	1.5	0	0	0	2.17±0.31^ab	长势正常
0.5	2.0	0	0	0	2.43±0.19^ab	长势正常
0.5	3.0	0	0	0	2.43±0.24^ab	长势正常
0.5	5.0	0	0	0	2.77±0.63^a	长势正常
0.5	8.0	0	0	0	0.93±0.35^def	长势正常
0.5	10.0	0	0	0	0.89±0.24^def	长势正常
0	0	0.1	0	0	0.50±0.04^efg	少数基部膨大
0	0	0.25	0	0	0.70±0.08^defg	部分基部膨大
0	0	0.5	0	0	0.92±0.16^def	多数基部膨大
0	0	1.0	0	0	0.81±0.10^def	多数基部膨大
0	0	0	0.5	0	0.23±0.07^fg	植株粗壮
0	0	0	1.0	0	0.27±0.02^fg	植株粗壮
0	0	0	2.0	0	0.50±0.15^efg	植株粗壮
0	0	0	3.0	0	0.49±0.16^efg	株植粗壮
0	0	0	5.0	0	1.07±0.19^de	植株粗壮
0	0	0	0	1	0^g	多数死亡
0	0	0	0	2	0^g	全部死亡
0	0	0	0	3	0^g	全部死亡
0	0	0	0	4	0^g	全部死亡