Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1179-1188.DOI: 10.3969/j.issn.1000-2561.2020.06.016
• Biotechnology and Tissue Culture • Previous Articles Next Articles
MO Yuanqi1,2,LI Hanwen3,JIANG Nan3,ZHENG Feng2,FANG Lin2,LI Lin2,ZHOU Yiqing2,WU Kunlin2,ZENG Songjun2,4,*()
Received:
2019-06-18
Revised:
2019-10-28
Online:
2020-06-25
Published:
2020-07-30
Contact:
ZENG Songjun
CLC Number:
MO Yuanqi,LI Hanwen,JIANG Nan,ZHENG Feng,FANG Lin,LI Lin,ZHOU Yiqing,WU Kunlin,ZENG Songjun. Tissue Culture and Rapid Propagation of Dendrobium Hibiki[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1179-1188.
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Fig. 1 Rapid propagation in vitro of Dendrobium Hibiki(bar=4 cm) A: D. Hibiki with flowers; B: Formation of axillary buds in modified MS+3.0mg/L 6-BA; C: Multiplication of clustered shoots in MS+5.0 mg/L 6-BA; D: Rooting on 1/2 MS+2.0mg/L NAA; E: Growth and rooting in 1/2MS + 100 g/L potato homogenate; F: Plantlets transplanted on the zhijin stone:peat soil=1:1 for two months
基质组合 Matrix combination | 移栽成活率 Survival rate of transplantation/% | 生长状况 Growth status | 基质组合 Matrix combination | 移栽成活率 Survival rate of transplantation/% | 生长状况 Growth status |
---|---|---|---|---|---|
植金石:松树皮1:1 | 88.89±2.22abcd | 15.56%叶宽增加,+++ | 兰石:松树皮1:1 | 51.11±2.22lm | 17.8%叶宽增加,+++ |
植金石:松树皮1:2 | 91.11±4.44abc | 13.33%叶宽增加,+++ | 兰石:松树皮1:2 | 48.89±2.22m | 11.11%叶宽增加,+++ |
植金石:松树皮1:3 | 82.22±2.22cdefg | 13.33%叶宽增加,++++ | 兰石:松树皮1:3 | 53.33±3.85lm | 15.56%叶宽增加,+++ |
植金石:椰壳1:1 | 95.56±4.44ab | 37.78%叶宽增加,+++++ | 兰石:椰壳1:1 | 86.67±6.68bcde | 24.45%叶宽增加,++ |
植金石:椰壳1:2 | 73.33±3.85fghi | 6.67%叶宽增加,++++ | 兰石:椰壳1:2 | 60.00±0.00jklm | 11.11%叶宽增加,++ |
植金石:椰壳1:3 | 55.56±8.01klm | 4.44%叶宽增加,++++ | 兰石:椰壳1:3 | 82.22±4.45cdefg | 4.44%叶宽增加,+ |
植金石:泥炭土1:1 | 95.55±2.22ab | 66.67%叶宽增加,+++++ | 兰石:泥炭土1:1 | 84.44±5.88bcdef | 5.56%叶宽增加,+++ |
植金石:泥炭土1:2 | 73.33±3.85fghi | 48.89%叶宽增加,++++ | 兰石:泥炭土1:2 | 66.67±3.85hijk | 40.00%叶宽增加,+++ |
植金石:泥炭土1:3 | 88.89±2.22abcd | 46.67%叶宽增加,+++ | 兰石:泥炭土1:3 | 82.22±2.22cdefg | 28.89%叶宽增加,+++ |
泥炭土:松树皮1:1 | 100.00±0.00a | 64.44%叶宽增加,++++ | 植金石 | 80.00±0.00cdefg | 46.67%叶宽增加,+++ |
泥炭土:松树皮1:2 | 84.44±4.44bcdef | 40.00%叶宽增加,+++ | 松树皮 | 62.23±2.23ijkl | 24.44%叶宽增加,++ |
泥炭土:松树皮1:3 | 75.56±4.44efgh | 37.78%叶宽增加,++++ | 椰壳 | 77.78±3.85defgh | 17.78%叶宽增加,++ |
泥炭土:椰壳1:1 | 82.22±2.22cdefg | 37.78%叶宽增加,++++ | 水苔 | 82.22±2.22cdefg | 46.67%叶宽增加,++++ |
泥炭土:椰壳1:2 | 77.78±4.45defgh | 26.67%叶宽增加,++++ | 泥炭土 | 83.34±3.34bcdefg | 53.33%叶宽增加,+++++ |
泥炭土:椰壳1:3 | 73.33±0.00fghi | 15.56%叶宽增加,++++ | 兰石 | 82.22±2.22cdefg | 53.33%叶宽增加,++ |
Tab. 7 The growth status of Dendrobium Hibiki plantlets transplanted for two month
基质组合 Matrix combination | 移栽成活率 Survival rate of transplantation/% | 生长状况 Growth status | 基质组合 Matrix combination | 移栽成活率 Survival rate of transplantation/% | 生长状况 Growth status |
---|---|---|---|---|---|
植金石:松树皮1:1 | 88.89±2.22abcd | 15.56%叶宽增加,+++ | 兰石:松树皮1:1 | 51.11±2.22lm | 17.8%叶宽增加,+++ |
植金石:松树皮1:2 | 91.11±4.44abc | 13.33%叶宽增加,+++ | 兰石:松树皮1:2 | 48.89±2.22m | 11.11%叶宽增加,+++ |
植金石:松树皮1:3 | 82.22±2.22cdefg | 13.33%叶宽增加,++++ | 兰石:松树皮1:3 | 53.33±3.85lm | 15.56%叶宽增加,+++ |
植金石:椰壳1:1 | 95.56±4.44ab | 37.78%叶宽增加,+++++ | 兰石:椰壳1:1 | 86.67±6.68bcde | 24.45%叶宽增加,++ |
植金石:椰壳1:2 | 73.33±3.85fghi | 6.67%叶宽增加,++++ | 兰石:椰壳1:2 | 60.00±0.00jklm | 11.11%叶宽增加,++ |
植金石:椰壳1:3 | 55.56±8.01klm | 4.44%叶宽增加,++++ | 兰石:椰壳1:3 | 82.22±4.45cdefg | 4.44%叶宽增加,+ |
植金石:泥炭土1:1 | 95.55±2.22ab | 66.67%叶宽增加,+++++ | 兰石:泥炭土1:1 | 84.44±5.88bcdef | 5.56%叶宽增加,+++ |
植金石:泥炭土1:2 | 73.33±3.85fghi | 48.89%叶宽增加,++++ | 兰石:泥炭土1:2 | 66.67±3.85hijk | 40.00%叶宽增加,+++ |
植金石:泥炭土1:3 | 88.89±2.22abcd | 46.67%叶宽增加,+++ | 兰石:泥炭土1:3 | 82.22±2.22cdefg | 28.89%叶宽增加,+++ |
泥炭土:松树皮1:1 | 100.00±0.00a | 64.44%叶宽增加,++++ | 植金石 | 80.00±0.00cdefg | 46.67%叶宽增加,+++ |
泥炭土:松树皮1:2 | 84.44±4.44bcdef | 40.00%叶宽增加,+++ | 松树皮 | 62.23±2.23ijkl | 24.44%叶宽增加,++ |
泥炭土:松树皮1:3 | 75.56±4.44efgh | 37.78%叶宽增加,++++ | 椰壳 | 77.78±3.85defgh | 17.78%叶宽增加,++ |
泥炭土:椰壳1:1 | 82.22±2.22cdefg | 37.78%叶宽增加,++++ | 水苔 | 82.22±2.22cdefg | 46.67%叶宽增加,++++ |
泥炭土:椰壳1:2 | 77.78±4.45defgh | 26.67%叶宽增加,++++ | 泥炭土 | 83.34±3.34bcdefg | 53.33%叶宽增加,+++++ |
泥炭土:椰壳1:3 | 73.33±0.00fghi | 15.56%叶宽增加,++++ | 兰石 | 82.22±2.22cdefg | 53.33%叶宽增加,++ |
培养基 Culture medium | 腋芽的诱导率 Induction rate of axillary buds/% | 腋芽起始萌动时间 Initial germination time of axillary buds/d | ||||
---|---|---|---|---|---|---|
上段 | 中段 | 下段 | 上段 | 中段 | 下段 | |
MS+3.0 mg/L 6-BA | 0k | 65.56±2.94fg | 70.37±3.70defg | 0j | 17.67±0.33d | 16.00±0.58ef |
改良MS | 0k | 71.48±3.29defg | 74.26±2.27cdef | 0j | 17.00±0.00de | 16.00±0.58ef |
改良MS+1.0mg/L 6-BA | 23.33±3.33h | 78.53±0.74bcd | 85.19±3.70ab | 22.67±0.33a | 17.00±0.00de | 16.00±0.58ef |
改良MS+3.0 mg/L 6-BA | 24.07±3.03h | 81.90±1.90bc | 91.90±4.23a | 22.00±0.00ab | 15.00±0.00fg | 15.00±0.00fg |
改良MS+5.0 mg/L 6-BA | 16.67±3.33ij | 75.19±2.59cde | 75.47±2.49cde | 21.00±0.58bc | 13.00±0.00h | 14.00±0.58gh |
改良MS+7.0 mg/L 6-BA | 10.37±0.37j | 64.45±2.22g | 68.06±3.68efg | 20.67±0.33c | 11.67±0.33i | 11.83±0.31i |
Tab. 1 Effects of 6-BA concentration on the induction of Dendrobium Hibiki axillary buds
培养基 Culture medium | 腋芽的诱导率 Induction rate of axillary buds/% | 腋芽起始萌动时间 Initial germination time of axillary buds/d | ||||
---|---|---|---|---|---|---|
上段 | 中段 | 下段 | 上段 | 中段 | 下段 | |
MS+3.0 mg/L 6-BA | 0k | 65.56±2.94fg | 70.37±3.70defg | 0j | 17.67±0.33d | 16.00±0.58ef |
改良MS | 0k | 71.48±3.29defg | 74.26±2.27cdef | 0j | 17.00±0.00de | 16.00±0.58ef |
改良MS+1.0mg/L 6-BA | 23.33±3.33h | 78.53±0.74bcd | 85.19±3.70ab | 22.67±0.33a | 17.00±0.00de | 16.00±0.58ef |
改良MS+3.0 mg/L 6-BA | 24.07±3.03h | 81.90±1.90bc | 91.90±4.23a | 22.00±0.00ab | 15.00±0.00fg | 15.00±0.00fg |
改良MS+5.0 mg/L 6-BA | 16.67±3.33ij | 75.19±2.59cde | 75.47±2.49cde | 21.00±0.58bc | 13.00±0.00h | 14.00±0.58gh |
改良MS+7.0 mg/L 6-BA | 10.37±0.37j | 64.45±2.22g | 68.06±3.68efg | 20.67±0.33c | 11.67±0.33i | 11.83±0.31i |
植物生长调节剂Plant growth regulator/(mg·L-1) | 不定芽增殖系数 Adventitious bud proliferation coefficient | 生长状况 Growth status | ||||
---|---|---|---|---|---|---|
NAA | 6-BA | TDZ | KT | 2,4-D | ||
0 | 0 | 0 | 0 | 0 | 0.29±0.02fg | 长势正常 |
0.5 | 0 | 0 | 0 | 0 | 1.41±0.29cd | 长势正常 |
0.5 | 0.5 | 0 | 0 | 0 | 1.80±0.33bc | 长势正常 |
0.5 | 1.0 | 0 | 0 | 0 | 1.90±0.21bc | 长势正常 |
0.5 | 1.5 | 0 | 0 | 0 | 2.17±0.31ab | 长势正常 |
0.5 | 2.0 | 0 | 0 | 0 | 2.43±0.19ab | 长势正常 |
0.5 | 3.0 | 0 | 0 | 0 | 2.43±0.24ab | 长势正常 |
0.5 | 5.0 | 0 | 0 | 0 | 2.77±0.63a | 长势正常 |
0.5 | 8.0 | 0 | 0 | 0 | 0.93±0.35def | 长势正常 |
0.5 | 10.0 | 0 | 0 | 0 | 0.89±0.24def | 长势正常 |
0 | 0 | 0.1 | 0 | 0 | 0.50±0.04efg | 少数基部膨大 |
0 | 0 | 0.25 | 0 | 0 | 0.70±0.08defg | 部分基部膨大 |
0 | 0 | 0.5 | 0 | 0 | 0.92±0.16def | 多数基部膨大 |
0 | 0 | 1.0 | 0 | 0 | 0.81±0.10def | 多数基部膨大 |
0 | 0 | 0 | 0.5 | 0 | 0.23±0.07fg | 植株粗壮 |
0 | 0 | 0 | 1.0 | 0 | 0.27±0.02fg | 植株粗壮 |
0 | 0 | 0 | 2.0 | 0 | 0.50±0.15efg | 植株粗壮 |
0 | 0 | 0 | 3.0 | 0 | 0.49±0.16efg | 株植粗壮 |
0 | 0 | 0 | 5.0 | 0 | 1.07±0.19de | 植株粗壮 |
0 | 0 | 0 | 0 | 1 | 0g | 多数死亡 |
0 | 0 | 0 | 0 | 2 | 0g | 全部死亡 |
0 | 0 | 0 | 0 | 3 | 0g | 全部死亡 |
0 | 0 | 0 | 0 | 4 | 0g | 全部死亡 |
Tab. 2 Effects of different plant growing regulators on the multiplication of Dendrobium Hibiki clustered shoots
植物生长调节剂Plant growth regulator/(mg·L-1) | 不定芽增殖系数 Adventitious bud proliferation coefficient | 生长状况 Growth status | ||||
---|---|---|---|---|---|---|
NAA | 6-BA | TDZ | KT | 2,4-D | ||
0 | 0 | 0 | 0 | 0 | 0.29±0.02fg | 长势正常 |
0.5 | 0 | 0 | 0 | 0 | 1.41±0.29cd | 长势正常 |
0.5 | 0.5 | 0 | 0 | 0 | 1.80±0.33bc | 长势正常 |
0.5 | 1.0 | 0 | 0 | 0 | 1.90±0.21bc | 长势正常 |
0.5 | 1.5 | 0 | 0 | 0 | 2.17±0.31ab | 长势正常 |
0.5 | 2.0 | 0 | 0 | 0 | 2.43±0.19ab | 长势正常 |
0.5 | 3.0 | 0 | 0 | 0 | 2.43±0.24ab | 长势正常 |
0.5 | 5.0 | 0 | 0 | 0 | 2.77±0.63a | 长势正常 |
0.5 | 8.0 | 0 | 0 | 0 | 0.93±0.35def | 长势正常 |
0.5 | 10.0 | 0 | 0 | 0 | 0.89±0.24def | 长势正常 |
0 | 0 | 0.1 | 0 | 0 | 0.50±0.04efg | 少数基部膨大 |
0 | 0 | 0.25 | 0 | 0 | 0.70±0.08defg | 部分基部膨大 |
0 | 0 | 0.5 | 0 | 0 | 0.92±0.16def | 多数基部膨大 |
0 | 0 | 1.0 | 0 | 0 | 0.81±0.10def | 多数基部膨大 |
0 | 0 | 0 | 0.5 | 0 | 0.23±0.07fg | 植株粗壮 |
0 | 0 | 0 | 1.0 | 0 | 0.27±0.02fg | 植株粗壮 |
0 | 0 | 0 | 2.0 | 0 | 0.50±0.15efg | 植株粗壮 |
0 | 0 | 0 | 3.0 | 0 | 0.49±0.16efg | 株植粗壮 |
0 | 0 | 0 | 5.0 | 0 | 1.07±0.19de | 植株粗壮 |
0 | 0 | 0 | 0 | 1 | 0g | 多数死亡 |
0 | 0 | 0 | 0 | 2 | 0g | 全部死亡 |
0 | 0 | 0 | 0 | 3 | 0g | 全部死亡 |
0 | 0 | 0 | 0 | 4 | 0g | 全部死亡 |
植物生长调节剂 Plant growing regulator/(mg·L-1) | 生根率 Rooting percentage/% | 株高 Plant height/cm | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量 Fresh weight increment/g | 生长状况 Growth status | |
---|---|---|---|---|---|---|---|
NAA | IBA | ||||||
0 | 0 | 100 | 3.61±0.06a | 3.77±0.15b | 1.29±0.17ab | 6.19±0.17d | 轻度褐化 |
0.5 | 0 | 100 | 2.86±0.32bc | 5.57±0.77a | 0.97±0.10ab | 9.90±0.25b | 轻度褐化 |
1.0 | 0 | 100 | 2.45±0.17cd | 5.23±0.33ab | 1.03±0.08ab | 11.15±0.44ab | 2%开花,轻度褐化 |
1.5 | 0 | 100 | 2.43±0.19cd | 4.30±0.25ab | 1.06±014ab | 11.77±0.80a | 2%开花,中度褐化 |
2.0 | 0 | 100 | 2.32±0.07cd | 3.90±0.23b | 0.82±0.06b | 12.02±0.61a | 3%开花,中度褐化 |
0 | 0.5 | 100 | 3.14±0.10ab | 4.87±0.34ab | 1.36±0.15a | 8.02±0.05c | 轻度褐化 |
0 | 1.0 | 100 | 2.84±0.13bc | 4.20±0.70ab | 1.30±0.31ab | 8.29±0.75c | 中度褐化 |
0 | 1.5 | 100 | 2.39±0.28cd | 4.77±0.18ab | 1.12±0.02ab | 8.39±0.49c | 2%开花,重度褐化 |
0 | 2.0 | 100 | 2.12±0.06d | 5.63±0.59a | 1.12±0.07ab | 10.95±0.33ab | 2%开花,重度褐化 |
Tab. 3 Effects of different concentrations of NAA and IBA on rooting of Dendrobium Hibiki
植物生长调节剂 Plant growing regulator/(mg·L-1) | 生根率 Rooting percentage/% | 株高 Plant height/cm | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量 Fresh weight increment/g | 生长状况 Growth status | |
---|---|---|---|---|---|---|---|
NAA | IBA | ||||||
0 | 0 | 100 | 3.61±0.06a | 3.77±0.15b | 1.29±0.17ab | 6.19±0.17d | 轻度褐化 |
0.5 | 0 | 100 | 2.86±0.32bc | 5.57±0.77a | 0.97±0.10ab | 9.90±0.25b | 轻度褐化 |
1.0 | 0 | 100 | 2.45±0.17cd | 5.23±0.33ab | 1.03±0.08ab | 11.15±0.44ab | 2%开花,轻度褐化 |
1.5 | 0 | 100 | 2.43±0.19cd | 4.30±0.25ab | 1.06±014ab | 11.77±0.80a | 2%开花,中度褐化 |
2.0 | 0 | 100 | 2.32±0.07cd | 3.90±0.23b | 0.82±0.06b | 12.02±0.61a | 3%开花,中度褐化 |
0 | 0.5 | 100 | 3.14±0.10ab | 4.87±0.34ab | 1.36±0.15a | 8.02±0.05c | 轻度褐化 |
0 | 1.0 | 100 | 2.84±0.13bc | 4.20±0.70ab | 1.30±0.31ab | 8.29±0.75c | 中度褐化 |
0 | 1.5 | 100 | 2.39±0.28cd | 4.77±0.18ab | 1.12±0.02ab | 8.39±0.49c | 2%开花,重度褐化 |
0 | 2.0 | 100 | 2.12±0.06d | 5.63±0.59a | 1.12±0.07ab | 10.95±0.33ab | 2%开花,重度褐化 |
活性炭 Activated carbon/(g·L-1) | 生根率 Rooting percentage/% | 株高 Plant height/cm | 叶片数 Number of leaves | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量 Fresh weight increment/g | 生长状况 Growth status |
---|---|---|---|---|---|---|---|
0 | 100 | 3.57±0.30b | 3.57±0.30b | 8.39±0.59a | 1.51±0.07c | 15.05±0.67a | +++ |
0.25 | 100 | 3.53±0.21b | 3.53±0.12b | 5.78±0.67b | 1.37±0.07c | 11.94±0.85b | ++++ |
0.50 | 100 | 3.78±0.08a | 3.78±0.08b | 7.78±0.82a | 1.74±0.05b | 11.69±0.55b | ++++ |
1.00 | 100 | 3.93±0.10ab | 3.93±0.10ab | 6.67±0.00a | 1.98±0.09a | 11.39±0.25b | ++++ |
1.50 | 100 | 4.37±0.10a | 4.37±0.17a | 5.00±0.17b | 1.72±0.03b | 11.07±0.37b | +++++ |
Tab. 4 Effect of activated carbon on rooting of Dendrobium Hibiki
活性炭 Activated carbon/(g·L-1) | 生根率 Rooting percentage/% | 株高 Plant height/cm | 叶片数 Number of leaves | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量 Fresh weight increment/g | 生长状况 Growth status |
---|---|---|---|---|---|---|---|
0 | 100 | 3.57±0.30b | 3.57±0.30b | 8.39±0.59a | 1.51±0.07c | 15.05±0.67a | +++ |
0.25 | 100 | 3.53±0.21b | 3.53±0.12b | 5.78±0.67b | 1.37±0.07c | 11.94±0.85b | ++++ |
0.50 | 100 | 3.78±0.08a | 3.78±0.08b | 7.78±0.82a | 1.74±0.05b | 11.69±0.55b | ++++ |
1.00 | 100 | 3.93±0.10ab | 3.93±0.10ab | 6.67±0.00a | 1.98±0.09a | 11.39±0.25b | ++++ |
1.50 | 100 | 4.37±0.10a | 4.37±0.17a | 5.00±0.17b | 1.72±0.03b | 11.07±0.37b | +++++ |
添加物 Additives/(g·L-1) | 生根率 Rooting rate/% | 株高 Plant height/cm | 叶片数 Number of leaves | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量Fresh weight increment/g | 生长状况 Growth status | |
---|---|---|---|---|---|---|---|---|
土豆泥 | 蔗糖 | |||||||
0 | 0 | 72.22±5.55b | 3.12±0.12a | 3.56±0.20b | 1.42±0.10e | 0.20±0.08d | 3.57±0.23c | ++ |
150 | 0 | 77.74±5.54b | 3.31±0.02a | 4.56±0.20a | 2.00±0.00de | 0.15±0.03d | 4.46±0.15c | +++ |
150 | 10 | 100.00±0.00a | 3.35±0.05a | 4.50±0.10a | 3.83±0.25cd | 0.25±0.03d | 8.40±0.51b | +++++ |
150 | 20 | 100.00±0.00a | 3.13±0.04a | 4.61±0.06a | 5.83±0.19c | 1.18±0.13c | 9.46±1.02b | +++++ |
150 | 30 | 100.00±0.00a | 3.23±0.05a | 4.14±0.07a | 8.39±0.59b | 1.51±0.07b | 15.05±0.67a | +++++ |
150 | 40 | 100.00±0.00a | 2.33±0.10b | 4.22±0.22a | 12.50±1.48a | 1.83±0.10a | 16.03±2.68a | ++++ |
Tab. 5 Effect of sucrose concentration on rooting of Dendrobium Hibiki
添加物 Additives/(g·L-1) | 生根率 Rooting rate/% | 株高 Plant height/cm | 叶片数 Number of leaves | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量Fresh weight increment/g | 生长状况 Growth status | |
---|---|---|---|---|---|---|---|---|
土豆泥 | 蔗糖 | |||||||
0 | 0 | 72.22±5.55b | 3.12±0.12a | 3.56±0.20b | 1.42±0.10e | 0.20±0.08d | 3.57±0.23c | ++ |
150 | 0 | 77.74±5.54b | 3.31±0.02a | 4.56±0.20a | 2.00±0.00de | 0.15±0.03d | 4.46±0.15c | +++ |
150 | 10 | 100.00±0.00a | 3.35±0.05a | 4.50±0.10a | 3.83±0.25cd | 0.25±0.03d | 8.40±0.51b | +++++ |
150 | 20 | 100.00±0.00a | 3.13±0.04a | 4.61±0.06a | 5.83±0.19c | 1.18±0.13c | 9.46±1.02b | +++++ |
150 | 30 | 100.00±0.00a | 3.23±0.05a | 4.14±0.07a | 8.39±0.59b | 1.51±0.07b | 15.05±0.67a | +++++ |
150 | 40 | 100.00±0.00a | 2.33±0.10b | 4.22±0.22a | 12.50±1.48a | 1.83±0.10a | 16.03±2.68a | ++++ |
添加物 Additives/(g·L-1) | 株高 Plant height/cm | 叶片数 Number of leaves | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量 Fresh weight increment/g | 生长状况 Growth status |
---|---|---|---|---|---|---|
0 | 3.23±0.12bc | 4.30±0.12abcd | 8.70±0.61def | 0.92±0.13c | 7.47±0.67bc | ++++ |
5%椰汁 | 2.81±0.14ef | 4.33±0.12abcd | 9.88±0.84bcde | 1.26±0.12abc | 6.82±0.32cde | ++++ |
10%椰汁 | 2.73±0.14efg | 4.57±0.28ab | 9.83±0.44bcde | 1.57±0.09a | 6.85±0.23cde | ++++ |
15%椰汁 | 3.00±0.13cde | 4.67±0.12a | 8.57±0.24def | 1.59±0.07a | 7.85±0.43bc | ++++ |
20%椰汁 | 2.71±0.15efg | 4.43±0.28abc | 8.57±0.32def | 1.54±0.06a | 9.55±0.44a | ++++ |
50 g/L香蕉泥 | 2.48±0.06fgh | 4.00±0.10cde | 11.17±1.39bc | 1.47±0.17a | 8.45±0.11b | ++++ |
100 g/L香蕉泥 | 2.16±0.06h | 3.67±0.12ef | 11.80±0.5b | 1.38±0.04ab | 10.40±0.12a | ++++ |
150 g/L香蕉泥 | 1.95±0.08h | 3.47±0.19f | 15.73±1.56a | 0.97±0.07bc | 7.49±0.03bc | ++ |
200 g/L香蕉泥 | 0i | 0g | 0g | 0d | 2.87±0.16f | + |
50 g/L土豆泥 | 3.21±0.04bcd | 4.63±0.03ab | 7.43±0.19ef | 1.26±0.01abc | 6.21±0.18de | ++++ |
100 g/L土豆泥 | 3.52±0.14b | 4.70±0.20a | 8.90±0.06cdef | 1.41±0.28ab | 9.53±0.07a | +++++ |
150 g/L土豆泥 | 3.88±0.08a | 4.63±0.12ab | 7.87±1.07def | 1.38±0.16ab | 7.20±0.11cd | +++++ |
200 g/L土豆泥 | 3.09±0.28cde | 4.40±0.10abcd | 6.58±0.13f | 1.34±0.06abc | 6.07±0.30e | +++ |
25 g/L苹果泥 | 2.85±0.09de | 4.50±0.15abc | 9.13±0.33cde | 1.46±0.20a | 6.97±0.33cde | ++ |
50 g/L苹果泥 | 2.42±0.01gh | 4.13±0.18bcde | 9.20±0.87cde | 1.66±0.25a | 7.60±0.24bc | ++ |
75 g/L苹果泥 | 2.42±0.07gh | 4.03±0.13cde | 10.23±0.37bcd | 1.49±0.12a | 7.78±0.64bc | +++ |
100 g/L苹果泥 | 2.39±0.03gh | 3.90±0.15def | 11.87±1.03b | 1.32±0.16abc | 6.92±0.07cde | +++ |
Tab. 6 Effects of organic amendments on rooting and transplantation of Dendrobium Hibiki
添加物 Additives/(g·L-1) | 株高 Plant height/cm | 叶片数 Number of leaves | 根数 Number of roots | 平均根长 Average root length/cm | 鲜重增量 Fresh weight increment/g | 生长状况 Growth status |
---|---|---|---|---|---|---|
0 | 3.23±0.12bc | 4.30±0.12abcd | 8.70±0.61def | 0.92±0.13c | 7.47±0.67bc | ++++ |
5%椰汁 | 2.81±0.14ef | 4.33±0.12abcd | 9.88±0.84bcde | 1.26±0.12abc | 6.82±0.32cde | ++++ |
10%椰汁 | 2.73±0.14efg | 4.57±0.28ab | 9.83±0.44bcde | 1.57±0.09a | 6.85±0.23cde | ++++ |
15%椰汁 | 3.00±0.13cde | 4.67±0.12a | 8.57±0.24def | 1.59±0.07a | 7.85±0.43bc | ++++ |
20%椰汁 | 2.71±0.15efg | 4.43±0.28abc | 8.57±0.32def | 1.54±0.06a | 9.55±0.44a | ++++ |
50 g/L香蕉泥 | 2.48±0.06fgh | 4.00±0.10cde | 11.17±1.39bc | 1.47±0.17a | 8.45±0.11b | ++++ |
100 g/L香蕉泥 | 2.16±0.06h | 3.67±0.12ef | 11.80±0.5b | 1.38±0.04ab | 10.40±0.12a | ++++ |
150 g/L香蕉泥 | 1.95±0.08h | 3.47±0.19f | 15.73±1.56a | 0.97±0.07bc | 7.49±0.03bc | ++ |
200 g/L香蕉泥 | 0i | 0g | 0g | 0d | 2.87±0.16f | + |
50 g/L土豆泥 | 3.21±0.04bcd | 4.63±0.03ab | 7.43±0.19ef | 1.26±0.01abc | 6.21±0.18de | ++++ |
100 g/L土豆泥 | 3.52±0.14b | 4.70±0.20a | 8.90±0.06cdef | 1.41±0.28ab | 9.53±0.07a | +++++ |
150 g/L土豆泥 | 3.88±0.08a | 4.63±0.12ab | 7.87±1.07def | 1.38±0.16ab | 7.20±0.11cd | +++++ |
200 g/L土豆泥 | 3.09±0.28cde | 4.40±0.10abcd | 6.58±0.13f | 1.34±0.06abc | 6.07±0.30e | +++ |
25 g/L苹果泥 | 2.85±0.09de | 4.50±0.15abc | 9.13±0.33cde | 1.46±0.20a | 6.97±0.33cde | ++ |
50 g/L苹果泥 | 2.42±0.01gh | 4.13±0.18bcde | 9.20±0.87cde | 1.66±0.25a | 7.60±0.24bc | ++ |
75 g/L苹果泥 | 2.42±0.07gh | 4.03±0.13cde | 10.23±0.37bcd | 1.49±0.12a | 7.78±0.64bc | +++ |
100 g/L苹果泥 | 2.39±0.03gh | 3.90±0.15def | 11.87±1.03b | 1.32±0.16abc | 6.92±0.07cde | +++ |
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