Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1167-1173.DOI: 10.3969/j.issn.1000-2561.2020.06.014

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Enzyme Activity Characteristics and Expression of Cu/Zn-SOD Gene in Tea Flowers

WANG Mengxin,WU Guohuo,CUI Lin,HAN Baoyu()   

  1. College of Life Sciences, China Metrology University / Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Hangzhou, Zhejiang 310018, China
  • Received:2019-05-19 Revised:2019-10-16 Online:2020-06-25 Published:2020-07-30
  • Contact: HAN Baoyu


In order to study the expression characteristics of Cu/Zn-SOD gene in the flowers within different developmental stages and various cultivars of tea plant flowers, and to discuss the correlationship between the expression of Cu/Zn-SOD gene and its enzyme activity, four developmental stages of cultivar ‘Longjing 43’ tea flower and 15 tea plant cultivars of fresh flowers were studied. The expression of three Cu/Zn-SOD genes was quantitatively analyzed by real-time fluorescent quantitative PCR techniques, and the enzyme activity of the three Cu/Zn-SOD was also determined simultaneously. The lowest Cu/Zn-SOD enzyme specific activity was found in the fourth stage, and the difference was significant when compared with the other developmental stages. The specific activity of Cu/Zn-SOD enzyme in different cultivars of tea flowers was significantly different. The Cu/Zn-SOD enzyme specific activity of ‘Zijuan’ tea flower was the highest, followed by ‘Huangjinya’ and ‘Baiye 1’. The activity of Cu/Zn-SOD enzyme of ‘Huangguanyin’ tea flower was the lowest. The expression level of Cu/Zn-SOD differed among the developmental stages, and the expression of cytoplasmic Cu/Zn-SOD1 gene was much higher than those of chloroplast type Cu/Zn-SOD2 and peroxisome Cu/Zn-SOD3. The expression of Cu/Zn-SOD in different cultivars was significantly different. Cu/Zn-SOD1 had the highest expression in cultivar ‘Huangmudan’ tea flower, Cu/Zn-SOD2 gene had the highest expression in cultivars ‘Baiye 1’ and ‘Huangguanyin’ tea flowers, and Cu/Zn-SOD3 gene had the highest expression in cultivar ‘Zijaun’ tea flowers. The result of correlation analysis showed that the specific activity of Cu/Zn-SOD had no correlation with Cu/Zn-SOD gene expression in different developmental stages of ‘Longjing 43’ tea flowers, but had considerable correlation with Cu/Zn-SOD3 gene expression in different tea cultivars. In this study, both tea cultivars and suitable plucking stage with high Cu/Zn-SOD activity were preliminarily expounded, which would provide references for the further research on SOD.

Key words: tea plant flowers, Cu/Zn-SOD gene, real time fluorescence quantitative PCR, Cu/Zn-SOD enzyme activity, cultivar ‘Longjing 43’

CLC Number: