Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1167-1173.DOI: 10.3969/j.issn.1000-2561.2020.06.014
• Biotechnology and Tissue Culture • Previous Articles Next Articles
WANG Mengxin,WU Guohuo,CUI Lin,HAN Baoyu()
Received:
2019-05-19
Revised:
2019-10-16
Online:
2020-06-25
Published:
2020-07-30
Contact:
HAN Baoyu
CLC Number:
WANG Mengxin,WU Guohuo,CUI Lin,HAN Baoyu. Enzyme Activity Characteristics and Expression of Cu/Zn-SOD Gene in Tea Flowers[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1167-1173.
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基因Gene | NCBI登录号 Accession in NCBI | 引物Primer | 序列Sequences |
---|---|---|---|
Cu/Zn-SOD1 | KR078346 | CSD1-F | ATGCTGGTGGAAGGATTG |
CSD1-R | ATCCCAAGTCTCAACCAAT | ||
Cu/Zn-SOD2 | KR078347 | CSD2-F | ACGAACTTAGCCTTTCCACT |
CSD2-R | CAAGCAAGTCACTAGTACACAG | ||
Cu/Zn-SOD3 | KR819175 | CSD3-F | AGGTGCAAGAGTCGGATGT |
CSD3-R | AGCGAGATGATATGAGGGTT | ||
GAPDH | GE651107 | GAPDH-F | TTGGCATCGTTGAGGGTCT |
GAPDH-R | CAGTGGGAACACGGAAAGC |
Tab. 1 Primer sequences for expression analysis of target genes
基因Gene | NCBI登录号 Accession in NCBI | 引物Primer | 序列Sequences |
---|---|---|---|
Cu/Zn-SOD1 | KR078346 | CSD1-F | ATGCTGGTGGAAGGATTG |
CSD1-R | ATCCCAAGTCTCAACCAAT | ||
Cu/Zn-SOD2 | KR078347 | CSD2-F | ACGAACTTAGCCTTTCCACT |
CSD2-R | CAAGCAAGTCACTAGTACACAG | ||
Cu/Zn-SOD3 | KR819175 | CSD3-F | AGGTGCAAGAGTCGGATGT |
CSD3-R | AGCGAGATGATATGAGGGTT | ||
GAPDH | GE651107 | GAPDH-F | TTGGCATCGTTGAGGGTCT |
GAPDH-R | CAGTGGGAACACGGAAAGC |
Fig. 3 Difference in activity of Cu/Zn-SOD in tea flowers of fifteen tea plants cultivars Different lowercase letters represent significant difference among different cultivars at 0.05 level.
Fig. 4 Expression analysis of Cu/Zn-SOD genes in four developmental stages of ‘Longjing43’ tea flowers Different lowercase letters represent significant difference among stages at 0.05 level.
Fig. 5 Expression levels of three Cu/Zn-SOD genes in four developmental stages of ‘Longjing43’ tea flowers Different lowercase letters represent significant difference among different genes at the same stages at 0.05 level.
Fig. 6 Difference in expression levels of three kinds of Cu/Zn-SOD genes in tea flowers of fifteen cultivars of tea plants Different lowercase letters represent significant differences among different cultivars at 0.05 level.
基因 Genes | 相关系数 Correlation coefficient | 显著性水平 Significance level |
---|---|---|
Cu/Zn-SOD1 | 0.035 | P>0.05 |
Cu/Zn-SOD2 | 0.185 | P>0.05 |
Cu/Zn-SOD3 | 0.555 | P<0.05 |
Tab. 2 Correlation between the expression of each Cu/Zn- SOD gene and Cu/Zn-SOD activity within the third developmental stage in tea flowers of fifteen tea cultivars
基因 Genes | 相关系数 Correlation coefficient | 显著性水平 Significance level |
---|---|---|
Cu/Zn-SOD1 | 0.035 | P>0.05 |
Cu/Zn-SOD2 | 0.185 | P>0.05 |
Cu/Zn-SOD3 | 0.555 | P<0.05 |
基因 Genes | 相关系数 Correlation coefficient | 显著性水平 Significance level |
---|---|---|
Cu/Zn-SOD1 | -0.789 | P>0.05 |
Cu/Zn-SOD2 | 0.702 | P>0.05 |
Cu/Zn-SOD3 | -0.177 | P>0.05 |
Tab. 3 Correlation between expression of every Cu/Zn- SOD gene with Cu/Zn-SOD activity in whole developmental stages of ‘Longjing 43’ tea flowers
基因 Genes | 相关系数 Correlation coefficient | 显著性水平 Significance level |
---|---|---|
Cu/Zn-SOD1 | -0.789 | P>0.05 |
Cu/Zn-SOD2 | 0.702 | P>0.05 |
Cu/Zn-SOD3 | -0.177 | P>0.05 |
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