Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1160-1166.DOI: 10.3969/j.issn.1000-2561.2020.06.013

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Reference Genes Selection for Quantitative Real-time PCR in Antrodia cinnamomea Mycelium of Different Development Stages

LI Jing1,WANG Zehui1,2,CHEN Li2,LIU Yanling1,XIA Shuning2,LIN Zhanxi1,*()   

  1. 1. China National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2019-03-27 Revised:2019-09-23 Online:2020-06-25 Published:2020-07-30
  • Contact: LIN Zhanxi


Quantitative Real-time PCR (qRT-PCR) technology is widely used in the analysis of gene expression because of its high sensit-ivity, high accuracy and high specificity. Therefore, it is important to select appropriate reference genes to measure the expression and accuracy of the sample. In this study, the expressions of 18 candidate internal reference genes of Actin, TPK, Floxuridine, CAP-Gly, α-Amylase, Phosphatase, HA2- helicase and MAGT1 were detected by qRT-PCR with A. cinnamomea Mycelium under 7, 21 and 35 d liquid fermentation time. The stability of the internal reference genes were analyzed using softwares of geNorm, NormFinder and BestKeeper. Floxuridine/ HA2-helicase, TPK, CAP-Gly genes were found more stable with geNorm. TPK, CAP-Gly, Floxuridine, HA2-helicase were found more stable with NormFinder. HA2-helicase was found to be best expressed with BestKeeper, followed by TPK and MAGT1. The comprehensive analysis showed that TPK and HA2-helicase had the best stability and were more suitable as the internal reference genes for A. camphorata. The results of this study would have a practical guiding role in the analysis of the selection of internal reference genes in the gene expression of A. cinnamomea by qRT-PCR.

Key words: Antrodia cinnamomea, reference gene, quantitative Real-time PCR

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