Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1154-1159.DOI: 10.3969/j.issn.1000-2561.2020.06.012

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

DNA Amount Estimation and Genome Survey of Sweetpotato Wild Relatives Ipomoea cordatotriloba

WANG Yao1,2,DENG Yitong2,DAI Xibin2,ZHANG An2,CAO Qinghe2,CHEN Yanli1,*()   

  1. 1. College of Horticulture, Hainan University, Haikou, Hainan 570228, China
    2. Jiangsu Xuhuai Regional Xuzhou Institute of Agricultural Sciences, Xuzhou, Jiangsu 221131, China
  • Received:2019-09-16 Revised:2019-10-25 Online:2020-06-25 Published:2020-07-30
  • Contact: CHEN Yanli


DNA content of sweetpotato wild relatives Ipomoea cordatotriloba was determinated by flow cytometry using soybean Williams 82 as the internal reference in this study. The ploidy was determined by chromosome counting. In order to verify the reliability of the results, the next-generation sequencing was used to further test and verify. It was a diploid species with base number was 15. The genome size was (539.69±13.76) Mb by the flow cytometry analysis. The C-value determined by sequencing was similar to the results of flow cytometry. Furthermore, the genomic heterozygosity rate, repeat sequence ratio and GC content was 0.40%, 57.93% and 38.10%, respectively. The above results would lay the foundation for the whole genome sequencing and assemble of this species, and provide references for the utilization of sweetpotato relatives.

Key words: sweetpotato, Ipomoea cordatotriloba, genome size, flow cytometry, sequencing

CLC Number: