DNA Amount Estimation and Genome Survey of Sweetpotato Wild Relatives Ipomoea cordatotriloba

doi:10.3969/j.issn.1000-2561.2020.06.012

Abstract

Abstract:

DNA content of sweetpotato wild relatives Ipomoea cordatotriloba was determinated by flow cytometry using soybean Williams 82 as the internal reference in this study. The ploidy was determined by chromosome counting. In order to verify the reliability of the results, the next-generation sequencing was used to further test and verify. It was a diploid species with base number was 15. The genome size was (539.69±13.76) Mb by the flow cytometry analysis. The C-value determined by sequencing was similar to the results of flow cytometry. Furthermore, the genomic heterozygosity rate, repeat sequence ratio and GC content was 0.40%, 57.93% and 38.10%, respectively. The above results would lay the foundation for the whole genome sequencing and assemble of this species, and provide references for the utilization of sweetpotato relatives.

Key words: sweetpotato, Ipomoea cordatotriloba, genome size, flow cytometry, sequencing

CLC Number:

S531
Q75

WANG Yao,DENG Yitong,DAI Xibin,ZHANG An,CAO Qinghe,CHEN Yanli. DNA Amount Estimation and Genome Survey of Sweetpotato Wild Relatives Ipomoea cordatotriloba[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1154-1159.

Figures/Tables 9

References 30

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项目 Item	总长度 Total length/bp	总数量 Total number	最大长度 Max length/bp	N50/bp	N90/bp
Contigs	466 005 979	978 566	67 068	2 488	144
Scaffolds	467 229 510	961 850	67 068	2 818	145

项目 Item	总长度 Total length/bp	总数量 Total number	最大长度 Max length/bp	N50/bp	N90/bp
Contigs	466 005 979	978 566	67 068	2 488	144
Scaffolds	467 229 510	961 850	67 068	2 818	145