Bioinformatics Analysis of Dehydrogenase Genes Family from the Blumea balsamifera (L.) DC.

doi:10.3969/j.issn.1000-2561.2020.06.011

Abstract

Abstract:

Four dehydrogenase genes, named as BbADH1, BbADH2, BbADH3, BbADH4, were cloned from Blumea balsamifera (L.) DC. by the reverse transcription polymerase chain reaction approach (RT-PCR) and rapid amplification of cDNA ends (RACE) andanalyzed by bioinformatics. The open reading frames of the four dehydrogenase sequences were all around 900 bp, and the isoelectric point (pI) of the proteins was 5.0-9.0. The most abundant amino acid of BbADH was leucine (Leu), and the least was tryptophan (Try). BbADH showed obvious hydrophobic and hydrophilic regions, no signal peptide at the N-terminus, and no transmembrane region. BbADH from B. balsamifera (L.) DC showed a high similarity with BbADH from other plants, containing the characteristic functional domain of dehydrogenase. Phylogenetic analysis indicated that BbADH1 and BbADH3 were in the same branch and were closely related to PeADH of Populus euphratica Oliv., while BbADH4 and BbADH2 were in different branches, and were closely related to VvADH of Vitis vinifera and NtADH of Nicotiana tabacum, respectively.

Key words: Blumea balsamifera (L.) DC, dehydrogenase (BbADH), amino acid sequence, conserved region, phylogenetic analysis

CLC Number:

BAI Lin,GUAN Lingliang,ZHA Ying,YU Fulai,WANG Kai,XIE Xiaoli,PANG Yuxin,CHEN Songbi. Bioinformatics Analysis of Dehydrogenase Genes Family from the Blumea balsamifera (L.) DC.[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1145-1153.

Figures/Tables 9

References 19

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编号 Number	引物名称 Primer name	引物序列 Primer sequence	用途 Purpose
1	BbADH1-5°RACE	5°-GTATCCTGACATTGTAGCAG-3°	BbADH1基因5°RACE克隆
2	BbADH1-3°RACE	5°-GTTGGCTGTACAATGTCTAC-3°	BbADH1基因3°RACE克隆
3	BbADH2-5°RACE	5°-GGAGCTTCTGGAGTCGTAGT-3°	BbADH2基因5°RACE克隆
4	BbADH2-3°RACE	5°-CTTCAAGTTCATCATTCCTC-3°	BbADH2基因3°RACE克隆
5	BbADH3-5°RACE	5°-TGTCACTCAAGCCTTGCTAG-3°	BbADH3基因5°RACE克隆
6	BbADH3-3°RACE	5°-TAAGAGCTGCTTTGGAGACG-3°	BbADH3基因3°RACE克隆
7	BbADH4-5°RACE	5°-TCTCCATAAGCAGAGCTAAC-3°	BbADH4基因5°RACE克隆
8	BbADH4-3°RACE	5°-TCGAGCAACCTTATGAACTT-3°	BbADH4基因3°RACE克隆
9	BbADH1-pf	5°-GTCTACAAACAAACTACTATGG-3°	BbADH1基因全长克隆
10	BbADH1-pr	5°-TCTTCTGTTA GGTTCTCAAC-3°	BbADH1基因全长克隆
11	BbADH2-pf	5°- TGCCTGGACACAAACTACTATG-3°	BbADH2基因全长克隆
12	BbADH2-pr	5°-CAGTTAAAGACTCGAGATCCCC-3°	BbADH2基因全长克隆
13	BbADH3-pf	5°- AAATATGTCGCCAACTTGCT-3°	BbADH3基因全长克隆
14	BbADH3-pr	5°-GATGTTGTATGCTAGTTGGATC-3°	BbADH3基因全长克隆
15	BbADH4-pf	5°- GTTACTTGATATCGAGAAGTT-3°	BbADH4基因全长克隆
16	BbADH4-pr	5°- CTTGTGTATGCATTAAGAGCT-3°	BbADH4基因全长克隆

编号 Number	引物名称 Primer name	引物序列 Primer sequence	用途 Purpose
1	BbADH1-5°RACE	5°-GTATCCTGACATTGTAGCAG-3°	BbADH1基因5°RACE克隆
2	BbADH1-3°RACE	5°-GTTGGCTGTACAATGTCTAC-3°	BbADH1基因3°RACE克隆
3	BbADH2-5°RACE	5°-GGAGCTTCTGGAGTCGTAGT-3°	BbADH2基因5°RACE克隆
4	BbADH2-3°RACE	5°-CTTCAAGTTCATCATTCCTC-3°	BbADH2基因3°RACE克隆
5	BbADH3-5°RACE	5°-TGTCACTCAAGCCTTGCTAG-3°	BbADH3基因5°RACE克隆
6	BbADH3-3°RACE	5°-TAAGAGCTGCTTTGGAGACG-3°	BbADH3基因3°RACE克隆
7	BbADH4-5°RACE	5°-TCTCCATAAGCAGAGCTAAC-3°	BbADH4基因5°RACE克隆
8	BbADH4-3°RACE	5°-TCGAGCAACCTTATGAACTT-3°	BbADH4基因3°RACE克隆
9	BbADH1-pf	5°-GTCTACAAACAAACTACTATGG-3°	BbADH1基因全长克隆
10	BbADH1-pr	5°-TCTTCTGTTA GGTTCTCAAC-3°	BbADH1基因全长克隆
11	BbADH2-pf	5°- TGCCTGGACACAAACTACTATG-3°	BbADH2基因全长克隆
12	BbADH2-pr	5°-CAGTTAAAGACTCGAGATCCCC-3°	BbADH2基因全长克隆
13	BbADH3-pf	5°- AAATATGTCGCCAACTTGCT-3°	BbADH3基因全长克隆
14	BbADH3-pr	5°-GATGTTGTATGCTAGTTGGATC-3°	BbADH3基因全长克隆
15	BbADH4-pf	5°- GTTACTTGATATCGAGAAGTT-3°	BbADH4基因全长克隆
16	BbADH4-pr	5°- CTTGTGTATGCATTAAGAGCT-3°	BbADH4基因全长克隆

物种名称Species name	相似性Similarity/%				序列名 Sequence name	登录号Accession ID
物种名称Species name	BbADH1	BbADH2	BbADH3	BbADH4	序列名 Sequence name	登录号Accession ID
青蒿素	82	86	80	79	AaADH	PWA85459.1
莴苣	82	85	81	74	LsADH	XP_023730777.1
向日葵	81	83	58	78	HaADH	XP_022017183.1
番茄	68	77	—	—	SlADH	NP_001316484.1
巨桉	69	75	55	—	EgADH	XP_010053674.1
葡萄	68	76	57	56	VvADH	XP_002267348.2
锦葵	70	69	—	—	GhADH	XP_016676893.1
亚洲棉	70	68	—	—	GaADH	XP_017647505.1
毛果杨	67	70	56	—	PtADH	XP_002301348.1
马铃薯	67	71	—	—	StADH	XP_006339532.1
木薯	68	71	—	57	MeADH	XP_021615637.1
橡胶树	68	72	54	61	HbADH	XP_021653042.1
可可树	69	—	54	—	TcADH	XP_017969593.1
辣椒	66	—	—	—	CaADH	PHT74473.1
红蔷薇	68	67	57	—	RcADH	PRQ57903.1
金丝小枣	68	70	54	54	ZjADH	XP_015888644.1
烟草	66	67	—	—	NtADH	XP_016443912.1
榴莲	68	69	55	56	DzADH	XP_022733902.1
桃	67	69	52	—	PpADH	ONI22969.1
梅花	65	71	55	—	PmADH	XP_008246061.1
胡杨	64	66	57	—	PeADH	XP_024464232.1
芝麻	68	73	—	—	SiADH	XP_011080505.1
苦瓜	—	72	—	—	McADH	XP_022157338.1
南天竹	—	—	60	56	NdADH	ACN87275.1

物种名称Species name	相似性Similarity/%				序列名 Sequence name	登录号Accession ID
物种名称Species name	BbADH1	BbADH2	BbADH3	BbADH4	序列名 Sequence name	登录号Accession ID
青蒿素	82	86	80	79	AaADH	PWA85459.1
莴苣	82	85	81	74	LsADH	XP_023730777.1
向日葵	81	83	58	78	HaADH	XP_022017183.1
番茄	68	77	—	—	SlADH	NP_001316484.1
巨桉	69	75	55	—	EgADH	XP_010053674.1
葡萄	68	76	57	56	VvADH	XP_002267348.2
锦葵	70	69	—	—	GhADH	XP_016676893.1
亚洲棉	70	68	—	—	GaADH	XP_017647505.1
毛果杨	67	70	56	—	PtADH	XP_002301348.1
马铃薯	67	71	—	—	StADH	XP_006339532.1
木薯	68	71	—	57	MeADH	XP_021615637.1
橡胶树	68	72	54	61	HbADH	XP_021653042.1
可可树	69	—	54	—	TcADH	XP_017969593.1
辣椒	66	—	—	—	CaADH	PHT74473.1
红蔷薇	68	67	57	—	RcADH	PRQ57903.1
金丝小枣	68	70	54	54	ZjADH	XP_015888644.1
烟草	66	67	—	—	NtADH	XP_016443912.1
榴莲	68	69	55	56	DzADH	XP_022733902.1
桃	67	69	52	—	PpADH	ONI22969.1
梅花	65	71	55	—	PmADH	XP_008246061.1
胡杨	64	66	57	—	PeADH	XP_024464232.1
芝麻	68	73	—	—	SiADH	XP_011080505.1
苦瓜	—	72	—	—	McADH	XP_022157338.1
南天竹	—	—	60	56	NdADH	ACN87275.1

不同物种ADHs Different species ADHs	保守区Ⅰ Conserved regionⅠ	保守区Ⅱ Conserved regionⅡ	保守区Ⅲ Conserved regionⅢ
	AVVTGANKGIG	VVFHQLDV	FGKLDILVNNG
菊科 AaADH		VIFHQLDV	FGKLDILVNNA
LsADH		VIFHQLDV	FGKLDILVNSG
HaADH			FGKLDILVNSG
BbADH1
BbADH2
BbADH3	AVVTGGNKGIG
BbADH4	AVVTGGNKGIG	VVFHQLDI	YKKLDILVNNG
桃金娘科 EqADH		IVFHQLDV
葡萄科 VvADH		LVFHQLDV
杨柳科 PeADH
巴豆亚科 HbADH		LVFHQLDV
MeADH		IVFHQLDV
蔷薇科 RcADH			IGKLDILVNNG
鼠李科 ZjADH
木棉科 DzADH	AVVTGSNKGIG
小檗科 NdADH	AVVTGSNKGIG