Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1145-1153.DOI: 10.3969/j.issn.1000-2561.2020.06.011

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Bioinformatics Analysis of Dehydrogenase Genes Family from the Blumea balsamifera (L.) DC.

BAI Lin1,2,GUAN Lingliang1,*(),ZHA Ying1,2,YU Fulai1,WANG Kai1,XIE Xiaoli1,PANG Yuxin1,CHEN Songbi1,*()   

  1. 1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Provincial Engineering Research Center for Blumea balsamifera / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Danzhou, Hainan 571737, China
    2. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
  • Received:2019-03-10 Revised:2019-10-14 Online:2020-06-25 Published:2020-07-30
  • Contact: GUAN Lingliang,CHEN Songbi


Four dehydrogenase genes, named as BbADH1, BbADH2, BbADH3, BbADH4, were cloned from Blumea balsamifera (L.) DC. by the reverse transcription polymerase chain reaction approach (RT-PCR) and rapid amplification of cDNA ends (RACE) andanalyzed by bioinformatics. The open reading frames of the four dehydrogenase sequences were all around 900 bp, and the isoelectric point (pI) of the proteins was 5.0-9.0. The most abundant amino acid of BbADH was leucine (Leu), and the least was tryptophan (Try). BbADH showed obvious hydrophobic and hydrophilic regions, no signal peptide at the N-terminus, and no transmembrane region. BbADH from B. balsamifera (L.) DC showed a high similarity with BbADH from other plants, containing the characteristic functional domain of dehydrogenase. Phylogenetic analysis indicated that BbADH1 and BbADH3 were in the same branch and were closely related to PeADH of Populus euphratica Oliv., while BbADH4 and BbADH2 were in different branches, and were closely related to VvADH of Vitis vinifera and NtADH of Nicotiana tabacum, respectively.

Key words: Blumea balsamifera (L.) DC, dehydrogenase (BbADH), amino acid sequence, conserved region, phylogenetic analysis

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