Effects of Exogenous ABA on Physiology of Four New Oil Palm Varieties under Cold Stress
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (6): 1124-1131.DOI: 10.3969/j.issn.1000-2561.2020.06.008

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Effects of Exogenous ABA on Physiology of Four New Oil Palm Varieties under Cold Stress

LIU Yanju1,2,ZHOU Lixia1,2,CAO Hongxing1,2,*()   

  1. 1. Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, China
    2. Hainan Key Biological Laboratory of Tropical Oil Crops, Wenchang, Hainan 571339, China
  • Received:2019-09-10 Revised:2019-10-23 Online:2020-06-25 Published:2020-07-30
  • Contact: CAO Hongxing

Abstract:

In order to provide theoretical basis for improving cold resistance of different oil palm varieties, the present experiment was conducted to investigate the expression characteristics of ABA synthase gene NCED3 of different oil palm varieties under cold stress and the growth and physiology effects of different ABA concentrations on cold resistance of different oil palm varieties. The new oil palm varieties Ni, C×N, B×E and D×N were used as the materials, treated with low temperature stress (15 ℃, 10 ℃, 5 ℃, 5 d). The expression characteristics of NCED3 in the seedlings were detected by real-time quantitative PCR (qPCR). Then, different concentrations of ABA (50-300 μmol/L ABA) were applied to the seedlings under 10 ℃, and the physiological indexes were determined, including soluble protein content, soluble sugar content, proline content, H2O2 content, MDA content, SOD and POD activity. Cold stress could trigger the overexpression of ABA synthase gene NCED3 in the seedlings. The application of 50-300 μmol/L ABA decreased the plasma membrane permeability and SOD activity of the seedlings, inhibited the increase of MDA and H2O2 levels, so the membrance lipid peroxidation caused by cold stress was relieved, soluble protein, soluble sugar content and POD activity were increased, and thus improved cold resistance of the seedlings. B×E had strong cold tolerance.

Key words: oil palm, ABA, cold stress

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