Effects of Exogenous ABA on Physiology of Four New Oil Palm Varieties under Cold Stress

doi:10.3969/j.issn.1000-2561.2020.06.008

Abstract

Abstract:

In order to provide theoretical basis for improving cold resistance of different oil palm varieties, the present experiment was conducted to investigate the expression characteristics of ABA synthase gene NCED3 of different oil palm varieties under cold stress and the growth and physiology effects of different ABA concentrations on cold resistance of different oil palm varieties. The new oil palm varieties Ni, C×N, B×E and D×N were used as the materials, treated with low temperature stress (15 ℃, 10 ℃, 5 ℃, 5 d). The expression characteristics of NCED3 in the seedlings were detected by real-time quantitative PCR (qPCR). Then, different concentrations of ABA (50-300 μmol/L ABA) were applied to the seedlings under 10 ℃, and the physiological indexes were determined, including soluble protein content, soluble sugar content, proline content, H₂O₂ content, MDA content, SOD and POD activity. Cold stress could trigger the overexpression of ABA synthase gene NCED3 in the seedlings. The application of 50-300 μmol/L ABA decreased the plasma membrane permeability and SOD activity of the seedlings, inhibited the increase of MDA and H₂O₂ levels, so the membrance lipid peroxidation caused by cold stress was relieved, soluble protein, soluble sugar content and POD activity were increased, and thus improved cold resistance of the seedlings. B×E had strong cold tolerance.

Key words: oil palm, ABA, cold stress

CLC Number:

LIU Yanju,ZHOU Lixia,CAO Hongxing. Effects of Exogenous ABA on Physiology of Four New Oil Palm Varieties under Cold Stress[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1124-1131.

Figures/Tables 4

References 27

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ABA浓度 Concentration of ABA/(μmol?L^-1)		株高生长量Height increment/cm	全株干质量 Dry weight of whole plant/g	根冠比 Root-shoot ratio
Ni	对照	2.35^aB	8.57^aB	0.57^aB
	0	1.13^dA	8.25^cB	0.38^cB
	50	1.45^cA	8.32^bA	0.39^cB
	100	1.56^cA	8.21^cB	0.45^bB
	200	1.81^bB	8.49^aB	0.44^bB
	300	1.61^cA	8.35^bB	0.42^bB
C×N	对照	2.01^aC	8.40^aB	0.54^aB
	0	1.25^bA	7.82^cC	0.43^bB
	50	1.31^bA	8.17^bA	0.35^cB
	100	1.69^aA	8.29^bB	0.39^bC
	200	1.67^aC	8.31^aB	0.42^bB
	300	1.83^aA	8.33^aB	0.35^cC
B×E	对照	2.75^aA	9.12^aA	0.67^aA
	0	1.29^eA	8.52^bA	0.52^bA
	50	1.53^dA	8.59^bA	0.47^cA
	100	1.74^cA	8.78^bA	0.53^bA
	200	2.05^bA	8.97^aA	0.55^bA
	300	1.87^cA	8.86^aA	0.51^bA
D×N	对照	1.79^aD	7.93^aC	0.42^aC
	0	0.85^dB	7.41^bD	0.36^bB
	50	0.98^dB	7.59^bB	0.33^bB
	100	1.14^cB	7.64^bC	0.37^bC
	200	1.39^bD	7.69^bC	0.35^bC
	300	1.15^cB	7.57^bC	0.34^bC

ABA浓度 Concentration of ABA/(μmol?L^-1)		株高生长量Height increment/cm	全株干质量 Dry weight of whole plant/g	根冠比 Root-shoot ratio
Ni	对照	2.35^aB	8.57^aB	0.57^aB
	0	1.13^dA	8.25^cB	0.38^cB
	50	1.45^cA	8.32^bA	0.39^cB
	100	1.56^cA	8.21^cB	0.45^bB
	200	1.81^bB	8.49^aB	0.44^bB
	300	1.61^cA	8.35^bB	0.42^bB
C×N	对照	2.01^aC	8.40^aB	0.54^aB
	0	1.25^bA	7.82^cC	0.43^bB
	50	1.31^bA	8.17^bA	0.35^cB
	100	1.69^aA	8.29^bB	0.39^bC
	200	1.67^aC	8.31^aB	0.42^bB
	300	1.83^aA	8.33^aB	0.35^cC
B×E	对照	2.75^aA	9.12^aA	0.67^aA
	0	1.29^eA	8.52^bA	0.52^bA
	50	1.53^dA	8.59^bA	0.47^cA
	100	1.74^cA	8.78^bA	0.53^bA
	200	2.05^bA	8.97^aA	0.55^bA
	300	1.87^cA	8.86^aA	0.51^bA
D×N	对照	1.79^aD	7.93^aC	0.42^aC
	0	0.85^dB	7.41^bD	0.36^bB
	50	0.98^dB	7.59^bB	0.33^bB
	100	1.14^cB	7.64^bC	0.37^bC
	200	1.39^bD	7.69^bC	0.35^bC
	300	1.15^cB	7.57^bC	0.34^bC

ABA浓度 Concentration of ABA/(μmol·L^-1)		可溶性蛋白含量 Soluble protein content/ (mg·g^-1 FW)	可溶性糖含量 Soluble sugar content/ (mmol·g^-1)	质膜透性 Membrane permeability/%	MDA含量 MDA content/ (μmol·g^-1)	脯氨酸含量 Proline content/ (μg·g^-1)	H₂O₂含量 H₂O₂content/ (μmol·g^-1)
Ni	对照	0.29^fB	37.92^eC	12.51^dA	9.80^fB	16.10^fB	69.48^bB
	0	0.50^eB	73.36^cC	32.69^aA	24.03^aA	39.69^aC	135.49^aA
	50	0.92^cB	83.03^aA	11.64^dC	15.46^dB	26.14^cB	50.07^cC
	100	0.87^dB	77.43^bA	12.87^cB	19.49^cA	23.15^dB	44.97^dC
	200	0.97^dB	69.85^dA	12.73^cB	13.12^eA	19.59^eA	28.64^eC
	300	1.10^aB	77.07b^cA	14.35^bB	20.33^bA	34.12^bB	40.07^dC
C×N	对照	0.30^eB	35.94^eC	11.47^eB	9.19^fB	19.11^dA	69.34^cB
	0	0.54^dB	72.08^aC	25.91^aB	18.01^aB	32.46^aD	132.93^aA
	50	0.81^cC	68.73^bB	23.51^bA	17.54^bA	25.77^bB	86.48^bB
	100	0.85^bB	65.52^cB	21.59^cA	13.15^dB	21.52^cB	60.7^dB
	200	0.92^aB	53.98^dC	17.41^dA	10.05^eB	14.69^eC	42.93^fA
	300	0.86^bC	54.22^dD	18.17^dA	14.25^cB	11.84^fC	53.64^eB
B×E	对照	0.34^dA	42.59^fB	10.62^dB	9.04^dB	20.24^eA	70.56^bB
	0	0.54^cB	82.13^aA	20.97^aC	15.90^aC	57.27^aA	98.50^aB
	50	1.24^bA	74.39^cB	13.36^cC	15.49^aB	26.28^dB	50.79^cC
	100	1.27^bA	75.08^bA	14.04^cB	14.02^bB	27.71^cA	47.46^dC
	200	1.70^aA	70.63^dA	8.02^eC	12.86^cA	17.31^fB	31.43^eB
	300	1.75^aA	69.82^eB	17.88^bA	13.61^bB	48.65^bA	32.82^eD
D×N	对照	0.36^fA	47.73^fA	13.23^dA	10.73^eA	16.82^dB	80.08^eA
	0	0.69^eA	79.41^aB	32.89^aA	16.84^aC	50.48^aB	132.57^aA
	50	0.89^cB	72.64^bB	18.62^bB	15.17^bB	35.95^bA	110.87^bA
	100	0.93^bB	67.82^cB	15.73^cB	13.79^cB	28.46^cA	106.69^cA
	200	0.98^aB	62.19^eB	11.51^eB	12.38^dA	15.05^eC	32.82^fB
	300	0.82^dC	63.38^dC	13.72^dB	14.87^bB	11.84^fC	96.67^dA

ABA浓度 Concentration of ABA/(μmol·L^-1)		可溶性蛋白含量 Soluble protein content/ (mg·g^-1 FW)	可溶性糖含量 Soluble sugar content/ (mmol·g^-1)	质膜透性 Membrane permeability/%	MDA含量 MDA content/ (μmol·g^-1)	脯氨酸含量 Proline content/ (μg·g^-1)	H₂O₂含量 H₂O₂content/ (μmol·g^-1)
Ni	对照	0.29^fB	37.92^eC	12.51^dA	9.80^fB	16.10^fB	69.48^bB
	0	0.50^eB	73.36^cC	32.69^aA	24.03^aA	39.69^aC	135.49^aA
	50	0.92^cB	83.03^aA	11.64^dC	15.46^dB	26.14^cB	50.07^cC
	100	0.87^dB	77.43^bA	12.87^cB	19.49^cA	23.15^dB	44.97^dC
	200	0.97^dB	69.85^dA	12.73^cB	13.12^eA	19.59^eA	28.64^eC
	300	1.10^aB	77.07b^cA	14.35^bB	20.33^bA	34.12^bB	40.07^dC
C×N	对照	0.30^eB	35.94^eC	11.47^eB	9.19^fB	19.11^dA	69.34^cB
	0	0.54^dB	72.08^aC	25.91^aB	18.01^aB	32.46^aD	132.93^aA
	50	0.81^cC	68.73^bB	23.51^bA	17.54^bA	25.77^bB	86.48^bB
	100	0.85^bB	65.52^cB	21.59^cA	13.15^dB	21.52^cB	60.7^dB
	200	0.92^aB	53.98^dC	17.41^dA	10.05^eB	14.69^eC	42.93^fA
	300	0.86^bC	54.22^dD	18.17^dA	14.25^cB	11.84^fC	53.64^eB
B×E	对照	0.34^dA	42.59^fB	10.62^dB	9.04^dB	20.24^eA	70.56^bB
	0	0.54^cB	82.13^aA	20.97^aC	15.90^aC	57.27^aA	98.50^aB
	50	1.24^bA	74.39^cB	13.36^cC	15.49^aB	26.28^dB	50.79^cC
	100	1.27^bA	75.08^bA	14.04^cB	14.02^bB	27.71^cA	47.46^dC
	200	1.70^aA	70.63^dA	8.02^eC	12.86^cA	17.31^fB	31.43^eB
	300	1.75^aA	69.82^eB	17.88^bA	13.61^bB	48.65^bA	32.82^eD
D×N	对照	0.36^fA	47.73^fA	13.23^dA	10.73^eA	16.82^dB	80.08^eA
	0	0.69^eA	79.41^aB	32.89^aA	16.84^aC	50.48^aB	132.57^aA
	50	0.89^cB	72.64^bB	18.62^bB	15.17^bB	35.95^bA	110.87^bA
	100	0.93^bB	67.82^cB	15.73^cB	13.79^cB	28.46^cA	106.69^cA
	200	0.98^aB	62.19^eB	11.51^eB	12.38^dA	15.05^eC	32.82^fB
	300	0.82^dC	63.38^dC	13.72^dB	14.87^bB	11.84^fC	96.67^dA

ABA浓度 Concentration of ABA/(μmol·L^-1)		品种Varieties
ABA浓度 Concentration of ABA/(μmol·L^-1)		Ni		C×N	B×E	D×N
SOD	对照	80.11^bA	85.92^bA		81.37^bA	82.74^bA
	0	120.37^aB	123.04^aB		135.29^aA	118.22^aB
	50	69.23^cB	81.01^cA		66.62^cB	80.53^bA
	100	64.88^dB	76.14^dA		65.36^cB	77.43^cA
	200	60.10^eB	71.71^eA		57.22^dB	75.29^cA
	300	69.82^cB	75.03^dA		58.72^dC	75.80^cA
POD	对照	124.07^aA	130.79^aA		123.37^aA	125.9^aA
	0	70.32^fC	88.61^cA		78.70^eB	70.59^eC
	50	101.38^cA	93.65^bC		103.60^cA	101.32^bA
	100	81.91^eB	77.91^eB		119.53^bA	68.53^eC
	200	93.61^dB	91.79^bB		98.03^dA	98.88^cA
	300	113.29^bA	81.71^dD		97.17^dB	90.90^dC