Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 1022-1029.DOI: 10.3969/j.issn.1000-2561.2020.05.024
• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles Next Articles
ZHANG Ming,DU Liqing(),MA Feiyue,SHUAI Xixiang,TU Xinghao
Received:
2019-05-21
Revised:
2019-09-02
Online:
2020-05-25
Published:
2020-06-15
Contact:
DU Liqing
CLC Number:
ZHANG Ming,DU Liqing,MA Feiyue,SHUAI Xixiang,TU Xinghao. Optimization of Ultrasonic-Assisted Extraction and Antioxidant Activity of Total Flavonoids from Macadamia Green Peel[J]. Chinese Journal of Tropical Crops, 2020, 41(5): 1022-1029.
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水平 level | A乙醇浓度 Ethanol content/% | B料液比 Solid-liquid ratio/(g·mL-1) | C提取温度 Extraction temperature/℃ | D提取时间 Extraction time/min |
---|---|---|---|---|
1 | 30 | 1:60 | 30 | 15 |
2 | 40 | 1:75 | 40 | 30 |
3 | 50 | 1:90 | 50 | 45 |
Tab. 1 Factors and levels of orthogonal experiment
水平 level | A乙醇浓度 Ethanol content/% | B料液比 Solid-liquid ratio/(g·mL-1) | C提取温度 Extraction temperature/℃ | D提取时间 Extraction time/min |
---|---|---|---|---|
1 | 30 | 1:60 | 30 | 15 |
2 | 40 | 1:75 | 40 | 30 |
3 | 50 | 1:90 | 50 | 45 |
Fig. 1 Effect of extraction methods on extraction yields of total flavonoids from macadamia green peel Different letters indicate significant difference at 0.05 level.
Fig. 2 Effect of ethanol content on the extraction yields of total flavonoids from macadamia green peel Different letters indicate significant difference at 0.05 level.
编号No. | A | B | C | D | 总黄酮提取量Extraction yields of total flavonoids /(mg·100 g-1) |
---|---|---|---|---|---|
1 | 1 | 1 | 1 | 1 | 1334.67 |
2 | 1 | 2 | 2 | 2 | 1476.67 |
3 | 1 | 3 | 3 | 3 | 1692.00 |
4 | 2 | 1 | 2 | 3 | 1481.34 |
5 | 2 | 2 | 3 | 1 | 1335.00 |
6 | 2 | 3 | 1 | 2 | 1272.00 |
7 | 3 | 1 | 3 | 2 | 1301.34 |
8 | 3 | 2 | 1 | 3 | 1401.67 |
9 | 3 | 3 | 2 | 1 | 1252.00 |
K1 | 4503.34 | 4117.35 | 4008.34 | 3921.67 | |
K2 | 4088.34 | 4213.34 | 4210.01 | 4050.01 | |
K3 | 3955.01 | 4216.00 | 4328.34 | 4575.01 | |
k1 | 1501.11 | 1372.45 | 1336.11 | 1307.22 | |
k2 | 1362.78 | 1404.45 | 1403.34 | 1350.00 | |
k3 | 1318.34 | 1405.33 | 1442.78 | 1525.00 | |
极差 | 182.77 | 32.88 | 106.23 | 217.78 |
Tab. 2 Results of orthogonal tests
编号No. | A | B | C | D | 总黄酮提取量Extraction yields of total flavonoids /(mg·100 g-1) |
---|---|---|---|---|---|
1 | 1 | 1 | 1 | 1 | 1334.67 |
2 | 1 | 2 | 2 | 2 | 1476.67 |
3 | 1 | 3 | 3 | 3 | 1692.00 |
4 | 2 | 1 | 2 | 3 | 1481.34 |
5 | 2 | 2 | 3 | 1 | 1335.00 |
6 | 2 | 3 | 1 | 2 | 1272.00 |
7 | 3 | 1 | 3 | 2 | 1301.34 |
8 | 3 | 2 | 1 | 3 | 1401.67 |
9 | 3 | 3 | 2 | 1 | 1252.00 |
K1 | 4503.34 | 4117.35 | 4008.34 | 3921.67 | |
K2 | 4088.34 | 4213.34 | 4210.01 | 4050.01 | |
K3 | 3955.01 | 4216.00 | 4328.34 | 4575.01 | |
k1 | 1501.11 | 1372.45 | 1336.11 | 1307.22 | |
k2 | 1362.78 | 1404.45 | 1403.34 | 1350.00 | |
k3 | 1318.34 | 1405.33 | 1442.78 | 1525.00 | |
极差 | 182.77 | 32.88 | 106.23 | 217.78 |
来源 Source | III型平方和 Type III of squares | df | 均方 Mean square | F | 显著性Significance |
---|---|---|---|---|---|
校正模型 | 307 920.610a | 8 | 38 490.076 | 23.326 | ** |
A | 66 081.818 | 2 | 33 040.909 | 20.023 | ** |
B | 4 934.667 | 2 | 2 467.333 | 1.495 | |
C | 34 302.083 | 2 | 17 151.042 | 10.394 | ** |
D | 119 909.300 | 2 | 59 954.650 | 36.333 | ** |
误差 | 14 851.121 | 9 | 1 650.125 |
Tab. 3 Variance analysis of orthogonal tests
来源 Source | III型平方和 Type III of squares | df | 均方 Mean square | F | 显著性Significance |
---|---|---|---|---|---|
校正模型 | 307 920.610a | 8 | 38 490.076 | 23.326 | ** |
A | 66 081.818 | 2 | 33 040.909 | 20.023 | ** |
B | 4 934.667 | 2 | 2 467.333 | 1.495 | |
C | 34 302.083 | 2 | 17 151.042 | 10.394 | ** |
D | 119 909.300 | 2 | 59 954.650 | 36.333 | ** |
误差 | 14 851.121 | 9 | 1 650.125 |
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