Identification and Biological Characteristics of Dalbergia odorifera Leaf Blight

doi:10.3969/j.issn.1000-2561.2020.05.022

Abstract

Abstract:

In recent years, the harmfulness of Dalbergia odorifera leaf blight has become more and more serious. In order to provide a basis for the control and comprehensive treatment of this disease, the pathogenic fungus causing the disease was isolated and identified and the biological characteristics were studied. In this study, the pathogenic fungus was isolated from the pathogen of D. odorifera leaf blight, and the morphological observation of the pathogenic fungi was carried out. And the rDNA-ITS sequence was obtained by sequencing. The pathogenic fungus was identified as Phomopsis asparagi. Through experiments on the growth medium, temperature, pH value and illumination of the pathogens, the mycelial growth of the fungus in PFA medium was the fastest. The mycelium could grow at 10-30 ℃, and the optimum temperature for mycelium growth was 25-30 ℃. The optimum pH for the mycelium growth was 6. Light had no significant effect on the growth of the pathogen.

Key words: Dalbergia odorifera, leaf blight, Phomopsis asparagi, identification, biological characteristics

CLC Number:

S763.15

CHEN Yao,LI Hangyu,ZHOU Deming. Identification and Biological Characteristics of Dalbergia odorifera Leaf Blight[J]. Chinese Journal of Tropical Crops, 2020, 41(5): 1007-1012.

Figures/Tables 10

Fig. 1 Pathogenic morphology A: Symptoms of leaf disease of Dalbergia odorifera; B: Conidia; C:Black particles on the diseased leaves; D、E: Hyphae in early stage and late stage of leaf blight.

Fig. 2 Results of back experiment A: Symptoms of leaf disease of Dalbergia odorifera; B: Re-isolation of strains growing for 3 days.

Fig. 3 Electrophoresis of rDNA ITS regions M: DL2000 Marker; 1-6: Strain JXHT16.

Fig. 4 Comparison of the ITS sequences between JXHT16 and JQ614001

Fig. 5 Molecular phylogenetic tree based on ITS sequences

Fig. 6 Effect of culture medium on mycelial growth Different lowercase letters indicate signicant difference at 0.05 level.

Tab. 1 Effect of culture medium on mycelial growth

培养基 Medium	均值 Average/cm	菌落颜色 Color	菌落形态 Morphology
PDA	4.11^abA	灰白色	致密
PSA	4.85^aA	灰白色	致密
PMA	3.25^abAB	灰白色	致密
PLA	2.68^bcAB	白色	较致密
PFA	4.94^aA	灰白色	致密
淀粉	2.12^cB	白色	较稀疏

Fig. 7 Effects of temperature on mycelium growth Different lowercase letters indicate signicant difference at 0.05 level.

Fig. 8 Effects of pH on mycelium growth Different lowercase letters indicate signicant difference at 0.05 level.

Fig. 9 Effects of sunlight on mycelium growth Different lowercase letters indicate signicant difference at 0.05 level.

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