Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 978-984.DOI: 10.3969/j.issn.1000-2561.2020.05.018

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Cloning and Expression Analysis of Mitochondrial Gene of Cytoplasmic Male Sterile Line CaNAD9 in Pepper (Capsicum annuum L.)

YANG Tingyu,SHAO Guifang,ZHANG Shui,WANG Jiao,ZHANG Jingrou,ZHAO Kai,DENG Minghua()   

  1. College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
  • Received:2019-08-01 Revised:2019-09-02 Online:2020-05-25 Published:2020-06-15
  • Contact: DENG Minghua

Abstract:

In order to further study the relationship between the cytoplasmic male sterility and energy metabolism in pepper, the cytoplasmic male sterile line 9704A and homologous maintainer line 9704B were used as the experimental materials to clone the mitochondrial genome CaNAD9 gene and compare its primary structure. The difference between the two materials was analyzed by real-time quantitative PCR to analyze the expression pattern of the gene in different tissues of the maintainer line 9704B and the expression differences of the buds of the cytoplasmic male sterile line 9704A and the homologous maintainer line 9704B. The CDS length of the CaNAD9 consisted of 573 bp, which encoding 190 amino acids. RNA editing of the CaNAD9 transcript did not happened in both 9704A and 9704B. The expression of CaNAD9 in pepper was different in different tissues with the highest expression in seeds and the lowest in fetuses. There were some differences in different developmental stages of the flower buds, and the difference in the expression of the cytoplasmic male sterile lines and the homologous maintainers in the meiotic stage of pollen mother cells was the most significant. This difference may cause abnormalities in the energy metabolism of the male sterile line, leading to male sterility in the pepper.

Key words: pepper (Capsicum annuum L.), CaNAD9, cytoplasmic male sterility, energy metabolism, gene expression

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