Abstract:

The cDNA-SRAP molecular marker technique was used to isolate the differentially expressed genes of the stems and leaves of Stylosanthes under low phosphorus stress, and bioinformatics analysis was carried out to study the molecular mechanism and reaction mechanism of Stylosanthes growing under low phosphorus condition. A total of 195 differential fragments were amplified from 8 pairs of primer combinations. Among them, there were 56 inhibitory expression fragments, 92 inducible expression fragments, 36 upregulated expression fragments and 11 down-regulated expression fragments, and the sizes ranged from 50 to 1000 bp. After the second amplification, the specific bands were recovered and sequenced. Through blast alignment, the homologous sequences of 14 different fragments were compared, and the 8 differentially expressed nucleotide sequences had high homology with known functional genes (JCVI-FLLj-1K4 unknown mRNA, NBS-LRR type disease resistance protein, ATP synthase, aldosterone reductase, chloroplast RF2), five sequences were highly homologous to the putative or predicted genes (UPF0051 protein, protease promoter, SCEI binding enzyme, indole-3-pyruvate monooxygenase). This study has laid a foundation for screening differential genes in response to low phosphorus stress.

Key words: Stylosanthes, phosphorus deficiency, homolog, cDNA-SRAP, differential expression