Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 964-970.DOI: 10.3969/j.issn.1000-2561.2020.05.016

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Cloning and Expression of Low Temperature Stress of DlICE1 in Dimocarpus longan Lour.

HUO Wen,LI Jiami,XU Xiaoping,LAI Ruilian,CHEN Xiaohui,LIN Yuling,CHEN Yukun,LAI Zhongxiong()   

  1. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 35002, China
  • Received:2019-04-28 Revised:2019-09-09 Online:2020-05-25 Published:2020-06-15
  • Contact: LAI Zhongxiong

Abstract:

In this experiment, the embryogenic callus of longan were used as the materials, the cDNA sequence of DlICE1 was cloned from longan embryogenic callus by the method of RACE-PCR and RT-PCR, then it was analyzed by bioinformatical methods and expression analysis under low temperature stress. The full-length sequence of DlICE1 was 2327 bp, which contained an 1614 bp open reading frame encoded 537 amino acids. The bioinformatics analysis indicated that DlICE1 belonged to the unstable acidic protein, which didn’t has a typical signal peptide and wasn’t a secreted protein; subcellular localization indicated that it is located in the nucleus, which was closest to the homologous protein of sweet orange. The qRT-PCR results indicated that the longan DlICE1 could effectively respond to low temperature stress and induce increased expression at low temperature. In summary, the study suggests that the longan DlICE1 gene may be involved in the cold resistance in longan embryogenic callus.

Key words: Dimocarpus longan Lour., embryogenic callus, DlICE1 gene, gene cloning, real-time fluorescent quantitative PCR

CLC Number: