Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 947-954.DOI: 10.3969/j.issn.1000-2561.2020.05.014

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Phylogeny and Expression Profile of Fatty Acyl-ACP Thioesterase (FAT) Gene Family in Cacao (Theobroma cacao L.)

LI Fupeng1,DENG Yunmei1,2,WU Baoduo1,QIN Xiaowei1,YAN Lin1,LAI Jianxiong1,*()   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs / Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
    2. College of Tropical Crop Science, Yunnan Agricultural University, Pu'er, Yunnan 665099, China
  • Received:2019-05-12 Revised:2019-09-02 Online:2020-05-25 Published:2020-06-15
  • Contact: LAI Jianxiong

Abstract:

Fatty acyl-ACP thioesterase (FAT) is the key enzyme regulating the synthesis of plant lipid. In order to better understand the characteristics of FAT gene family in Theobroma cacao, six novel FAT genes were identified from economically important cacao trees, designated as TcFATA, TcFATB1, TcFATB2, TcFATB3, TcFATB4, TcFATB5, respectively. Sequences analyses revealed that CDS of TcFATs was 1128-1263 bp, containing 6-7 exons. The molecular weight of the six predicted protein was 42.72-46.47 kDa, and pI of the proteins was 6.57-9.10. FAT family could be divided into FATA (one TcFATA) and FATB (five TcFATBs) subfamily. The expression patterns of the genes were investigated via real-time PCR in various developmental phases. The transcription level of TcFATA and TcFATB1 was continuously decreased along with pod development. Furthermore, the expression level of TcFATA and TcFATB1 was positively associated with the ratio of oleic acid (C18:1) and palmitic acid (C16:0) respectively. The results indicate that TcFATA and TcFATB1 are significantly related with the regulation of fatty acid component.

Key words: Theobroma cacao, fatty acyl-ACP thioesterase (FAT), phylogenetic, expression profile

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