Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 939-946.DOI: 10.3969/j.issn.1000-2561.2020.05.013

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Ca2+/CaM Signaling Involved in Salicylic Acid-Induced Glycine Betaine Accumulation in Jatropha curcas L. under Osmotic Stress

YANG Shuanglong,YANG Ting,GONG Ming()   

  1. College of Life Sciences / Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education / Key Laboratory of Biomass Energy and Environmental Biotechnology, Yunnan Normal University, Kunming, Yunnan 650500, China
  • Received:2019-08-26 Revised:2019-09-28 Online:2020-05-25 Published:2020-06-15
  • Contact: GONG Ming


Hydroponic experiments were carried out to study the effect of exogenous salicylic acid (SA) on the content of glycine betaine (GB), the activity of the key enzyme betaine aldehyde dehydrogenase (BADH) of GB biosynthesis, and the expression of GB metabolism-related genes in the leaves of Jatropha curcas seedlings under PEG 6000 stress. Meanwhile, the effects of Ca 2+ and calmodulin (CaM) antagonists on SA-induced GB accumulation were investigated too. SA treatment significantly enhanced the content of GB, increased the activity of BADH, and improved the expression level of JcCMO and JcBADH in the leaves of J. curcas seedlings under osmotic stress. Interestingly, compared to the seedlings exposed to PEG treatment alone, SA treatment significantly increased the activity of CaM under osmotic stress. Calcium chloride (CaCl2) treatment could promote SA-induced GB accumulation. It also induced an increase of BADH activity, and up-regulated JcBADH expression. The results indicate that exogenous SA treatment can enhance the biosynthesis of GB, and Ca 2+/CaM signaling might be involved in the regulation of SA-induced GB accumulation.

Key words: salicylic acid, osmotic stress, glycine betaine, Jatropha curcas L., Ca2+/CaM signaling

CLC Number: