Abstract:

In order to understand the genetic diversity and to provide a basis for the rational utilization of A. heimuer resource in Guangxi, the characters of mycelium and fruit body of ten wild strains from Baise and nine cultivars strains of A. heimuer were determined, and the genetic variation among of the strains was analyzed by the ISSR technique. The 19 strains exhibited high diversity in the characters of mycelium and fruit body. There were evident differences between the wild strains and the cultivar strains. The former mostly produced pigment early during mycelium culture, and the fruit body was light colored, semitranslucent, soft, tender, low chewiness in the textur, while the latter produced pigment at late stage of mycelium culture or no pigment, and the fruit body was dark colored, opaque, and springy, higher chewiness in the texture. ISSR analysis showed that the similarity coefficient was ranged from 0.51 to 0.90 among the 19 strains which could be divided into five groups at the similarity coefficient of 0.69. The strains in group I and group III were all wild strains, which had a similarity coefficient 0.69-0.74 and 0.69-0.77, respectively, the strains in group II and group IV were all cultivar strains which had a similarity coefficient 0.80-0.86 and 0.85-0.90, respectively, and the strain in the group V was only the wild strain BY81. All results suggested that A. heimuer strains in Guangxi possessed high genetic diversity. There were higher genetic variations among the wild strains from Baise than that among cultivar strains. There was also high genetic variation between the wild strains and cultivar strains, which should belong to different ecological groups. A. heimuer in Baise was a precious resource waiting to be exploited.

Key words: Auricularia heimuer in Baise, wild strains, cultivar strains, ISSR, genetic variation