Effects of Simulated Salinization on Seed Germination and Physiological Characteristics of Muskmelon Seedlings

doi:10.3969/j.issn.1000-2561.2020.05.010

Abstract

Abstract:

Using muskmelon cultivar ‘Xinyinhui’ as the material, the effects of salinization on the germination of muskmelon seeds were observed after treated by NaCl and NaHCO₃ alone and combined. At the same time, some physiological and biochemical indexes related to saline-alkali stress were measured. The results showed that the simulated salinization of NaCl, NaHCO₃, NaCl and NaHCO₃ could inhibit seed germination. The inhibition increased with the increase of stress concentration and the extension of stress time. Only low concentration (50 mmol/L) of NaCl showed a certain promoting effect. The activities of superoxide dismutase (SOD) and catalase (CAT) first increased, then decreased, and then turned to an upward trend during the germination of muskmelon seeds treated with NaCl and NaHCO₃. Peroxidase (POD) activity increased first and then decreased. Proline (Pro) content continued to rise, while soluble sugar content continued to decline. Malondialdehyde (MDA) content was always significantly higher than that of the control group treated with distilled water. Starch content decreased with the extension of treatment time. The activity of α-amylase and β-amylase decreased first and then increased. Generally speaking, simulated salinization significantly inhibited the seed germination and seedling growth of muskmelon, and protective enzymes and starch metabolism significantly participated in the response to salt-alkali stress. The results would help to reveal the response mechanism of muskmelon seeds to salinization stress during germination, and provide theoretical and practical references for facility seedling raising and plant cultivation in saline-alkali environment.

Key words: muskmelon, salinization, seed germination, protective enzyme, starch metabolism

CLC Number:

S652

ZHANG Beibei,FAN Jiaru,WANG Jingrong,HAN Xiaoyun,YUAN Huimin,Gefu WANG-PRUSKI,ZHANG Zhizhong. Effects of Simulated Salinization on Seed Germination and Physiological Characteristics of Muskmelon Seedlings[J]. Chinese Journal of Tropical Crops, 2020, 41(5): 912-920.

Figures/Tables 8

References 44

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NaCl浓度 NaCl concentration (mmol·L^-1)	发芽势Germination energy/%	发芽率 Germination rate/%	发芽指数Germination index	活力指数Vital index
0	86.67±0.033^a	86.67±0.033^ab	16.37±0.770^a	0.12±0.003^a
50	90.00±0.058^a	92.50±0.033^a	11.55±0.130^b	0.12±0.007^a
100	83.33±0.033^a	86.67±0.255^a	9.36±0.050^b	0.09±0.002^b
150	73.33±0.050^b	80.00±0.050^b	8.35±1.200^b	0.07±0.005^c
200	0.00±0.033^c	76.67±0.030^a	8.34±0.100^b	0.05±0.003^d
250	0.00±0.000^c	0.00±0.000^b	0.00±0.000^c	0.00±0.000^c

NaCl浓度 NaCl concentration (mmol·L^-1)	发芽势Germination energy/%	发芽率 Germination rate/%	发芽指数Germination index	活力指数Vital index
0	86.67±0.033^a	86.67±0.033^ab	16.37±0.770^a	0.12±0.003^a
50	90.00±0.058^a	92.50±0.033^a	11.55±0.130^b	0.12±0.007^a
100	83.33±0.033^a	86.67±0.255^a	9.36±0.050^b	0.09±0.002^b
150	73.33±0.050^b	80.00±0.050^b	8.35±1.200^b	0.07±0.005^c
200	0.00±0.033^c	76.67±0.030^a	8.34±0.100^b	0.05±0.003^d
250	0.00±0.000^c	0.00±0.000^b	0.00±0.000^c	0.00±0.000^c

NaHCO₃浓度 NaHCO₃ concentration/ (mmol·L^-1)	发芽势 Germination energy/%	发芽率 Germination rate/%	发芽指数Germination index	活力指数Vital index
0	86.67±3.330^a	96.67±3.330^a	14.51±0.964^a	0.18±0.024^a
30	81.67±6.670^a	83.33±6.670^a	13.28±1.063^a	0.06±0.004^b
60	76.67±3.330^a	80.00±5.780^a	8.73±0.540^b	0.05±0.003^b
90	0.00±0.000^b	76.67±8.820^a	6.23±1.069^b	0.045±0.007^b
120	0.00±0.000^b	0.00±0.000^b	0.00±0.00	0.00±0.000^c

NaHCO₃浓度 NaHCO₃ concentration/ (mmol·L^-1)	发芽势 Germination energy/%	发芽率 Germination rate/%	发芽指数Germination index	活力指数Vital index
0	86.67±3.330^a	96.67±3.330^a	14.51±0.964^a	0.18±0.024^a
30	81.67±6.670^a	83.33±6.670^a	13.28±1.063^a	0.06±0.004^b
60	76.67±3.330^a	80.00±5.780^a	8.73±0.540^b	0.05±0.003^b
90	0.00±0.000^b	76.67±8.820^a	6.23±1.069^b	0.045±0.007^b
120	0.00±0.000^b	0.00±0.000^b	0.00±0.00	0.00±0.000^c

不同处理 Different treatments	发芽势Germination energy/%	发芽率 Germination rate/%	发芽指数Germination index	活力指数Vital index	肧根长 Root length/cm	胚轴长 Hypocotyl length/cm	鲜重 Fresh weight/g
CK	80.00±6.670^a	93.33±3.330^a	19.80±0.128^a	2.13±0.059^a	6.78±0.537^a	5.97±0.660^a	0.11±0.005^a
A	76.67±3.330^a	83.33±3.330^ab	12.68±0.630^b	0.58±0.017^b	1.37±0.318^b	1.54±0.240^b	0.46±0.005^b
B	73.33±3.330^a	76.66±3.330^b	11.96±0.240^b	0.49±0.028^b	0.66±0.060^c	0.47±0.090^c	0.041±0.003^c
C	0.00±0.000^b	0.00±0.000^c	0.00±0.000^c	0.00±0.000^c	0.00±0.000^d	0.00±0.000^d	0.00±0.000^d