Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 901-911.DOI: 10.3969/j.issn.1000-2561.2020.05.009

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Salt Tolerance Analysis of F2 Generation Population of Switchgrass Based on Salt Tolerance Trait Index STTI

LIU Yiming1,KONG Danyu2,LIU Xiaohui3,ZHANG Xunzhong4,ZHAO Bingyu5,*(),LIU Guodao1,*()   

  1. 1. Tropical Crop Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture & Rural Affairs, Danzhou, Hainan 571737, China
    2. College of Tropical Crops, Hainan University, Danzhou, Hainan 571737, China
    3. College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
    4. Department of Crop and Soil Environmental Science, Virginia Tech, Blacksburg, Virginia 24061, USA
    5. Department of Horticulture, Virginia Tech, Blacksburg Virginia 24061, USA
  • Received:2019-06-20 Revised:2019-08-12 Online:2020-05-25 Published:2020-06-15
  • Contact: ZHAO Bingyu,LIU Guodao

Abstract:

Cross-breeding is one of important ways to breed new varieties of plants, and it is essential to analyze the performance of hybrid F2 generation. In this study, 53 F2 generation plants of Panicum virgatum were used as the research material. The parents Alamo, Dacotah and F1 generation DA90 and DA120 were used as the reference. Salt tolerance performance was evaluated with sand culture method under 250 mmol/L NaCl stress conditions. The electrolyte leakage rate (EL), relative water content (RWC), dry weight (DW), net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) were measured after 24 days of salt treatment. Principal component analysis and membership function analysis of salt tolerance trait index (STTI) showed that there were significant differences in salt tolerance between different F2 plants. Parent Alamo was relatively salt tolerant, Dacotah was sensitive to salt, DA90 and DA120 had moderate salt tolerance, and the F2 generation had significant segregation in salt tolerance. Six F2 plants were better in salt tolerance than Alamo. Seven F2 plants were worse in salt tolerance than Dacotah. Three principal components with a cumulative contribution rate of 87.47% were selected by the principal component analysis. The 57 test plants were divided into five categories by cluster analysis. Membership function indicated that F2 generations 39, 45, 27, 48, 35, 53 and 7 had higher salt tolerance, which would build up a good basis for the construction of the genetic map of the salt tolerance-related traits, the QTL mapping and the salt-tolerant breeding of switchgrass.

Key words: switchgrass (Panicum virgatum), salt tolerance, STTI, F2 generation

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