Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (5): 888-892.DOI: 10.3969/j.issn.1000-2561.2020.05.007

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Optimization of Chromosome Mounting Technique and Karyotype Analysisi of Cinnamomum kanehirae Hay of Sharp Leaves

GUAN Jinyan,LUO Qingwen,TAN Jiana,HUANG Haiying,WEN Mingfu,LUO Jianpiao()   

  1. Guangdong key Lab of Sugarcane Improvement & Biorefinery / Guandong Provincial Bioengingeering Institute Guangzhou Sugarcane Industry Research Institute, Guangzhou, Guangdong 510316, China
  • Received:2019-08-20 Revised:2019-10-14 Online:2020-05-25 Published:2020-06-15
  • Contact: LUO Jianpiao

Abstract:

In order tooptimize the chromosome mounting technique and explore the chromosome karyotype of C. kanehirae Hay, and to provide important cytological evidences for the study of the evolution, evolutionary characteristics and genetic regularity of camphor plants, the effects of differentpretreatment methods and enzymatic hydrolysis time on the production of C. kanehirae were discussed by the enzymatic dissociation wall low permeability method. The observation showed that pretreated in 0.002 mol/L 8-hydroxyl for 2 h, dissociated in 4% cellulase and 5% pectinase for 5 h could achieve the optimal experimental results. The karyotype analysis showed that the chromosome number was 24, with a karyotype formula 2n=2x=24=22m+2sm. The results demonstrated that the asymmetry index was 55.81%, the karyotype was 2B type, suggesting that C. kanehirae Hay maybe a relatively primitive species.

Key words: Cinnamomum kanehirae Hay of sharp leaves, root tip, chromosome preparation, karyotype analysis

CLC Number: