Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (4): 829-836.DOI: 10.3969/j.issn.1000-2561.2020.04.026
• Ecology • Previous Articles Next Articles
ZHANG Chuanjin1,REN Kuiyu1,GUO Shuang1,PANG Shichan1,WANG Shuaishuai1,YANG Shangdong1,2,*()
Received:
2019-05-23
Revised:
2019-09-15
Online:
2020-04-25
Published:
2020-05-09
Contact:
YANG Shangdong
CLC Number:
ZHANG Chuanjin,REN Kuiyu,GUO Shuang,PANG Shichan,WANG Shuaishuai,YANG Shangdong. Soil Biological Properties in Rhizosphere of Pea (Pisum sativum L.) Improved by Magnetic Treatments[J]. Chinese Journal of Tropical Crops, 2020, 41(4): 829-836.
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处理 Treatment | 细菌 Bacteria/ (106 CFU·g-1) | 真菌 Fungi/ (104 CFU·g-1) | 放线菌Actinomycetes/ (106 CFU·g-1) |
---|---|---|---|
CK | 11.67±0.23c | 4.20±0.19b | 15.86±0.45a |
A | 10.33±0.31d | 4.03±0.24c | 14.60±0.43d |
B | 11.26±0.47c | 3.97±0.34c | 14.67±0.39cd |
C | 14.12±0.56a | 4.77±0.36a | 15.71±0.37b |
D | 12.67±0.22b | 4.54±0.39a | 15.34±0.27c |
Tab. 1 Amounts soil cultivable microorganisms in rhizosphere of peas under different magnetic treatments
处理 Treatment | 细菌 Bacteria/ (106 CFU·g-1) | 真菌 Fungi/ (104 CFU·g-1) | 放线菌Actinomycetes/ (106 CFU·g-1) |
---|---|---|---|
CK | 11.67±0.23c | 4.20±0.19b | 15.86±0.45a |
A | 10.33±0.31d | 4.03±0.24c | 14.60±0.43d |
B | 11.26±0.47c | 3.97±0.34c | 14.67±0.39cd |
C | 14.12±0.56a | 4.77±0.36a | 15.71±0.37b |
D | 12.67±0.22b | 4.54±0.39a | 15.34±0.27c |
处理 Treat- ment | 微生物生物量碳 Microbial biomass C | 微生物生物量氮 Microbial biomass N | 微生物生物量磷 Microbial biomass P |
---|---|---|---|
CK | 145.70±3.62c | 15.00±0.14e | 191.80±3.11d |
A | 148.90±4.85c | 17.10±0.26c | 177.20±4.59e |
B | 158.70±5.13b | 16.30±0.41d | 238.90±4.88b |
C | 195.30±5.22a | 21.90±0.39a | 276.50±4.96a |
D | 162.10±4.09b | 18.20±0.27b | 219.70±2.33c |
Tab. 2 Microbial biomass C, N and P in rhizosphere soil under different treatments mg·kg-1
处理 Treat- ment | 微生物生物量碳 Microbial biomass C | 微生物生物量氮 Microbial biomass N | 微生物生物量磷 Microbial biomass P |
---|---|---|---|
CK | 145.70±3.62c | 15.00±0.14e | 191.80±3.11d |
A | 148.90±4.85c | 17.10±0.26c | 177.20±4.59e |
B | 158.70±5.13b | 16.30±0.41d | 238.90±4.88b |
C | 195.30±5.22a | 21.90±0.39a | 276.50±4.96a |
D | 162.10±4.09b | 18.20±0.27b | 219.70±2.33c |
处理 Treatment | β-葡萄糖苷酶 β-Glucosidase | 氨肽酶 Aminopeptidase | 磷酸酶 Phosphatase |
---|---|---|---|
CK | 1.26±0.14c | 9.40±0.23e | 1.32±0.06d |
A | 1.23±0.09c | 9.47±0.27d | 1.16±0.07e |
B | 1.19±0.07d | 10.35±0.22c | 1.61±0.11b |
C | 1.57±0.15a | 11.63±0.19a | 1.74±0.09a |
D | 1.35±0.10b | 10.69±0.16b | 1.51±0.08c |
Tab. 3 Rhizosphere soil enzyme activity about C、N、P under different treatments nmol·g-1·min-1(30 ℃)
处理 Treatment | β-葡萄糖苷酶 β-Glucosidase | 氨肽酶 Aminopeptidase | 磷酸酶 Phosphatase |
---|---|---|---|
CK | 1.26±0.14c | 9.40±0.23e | 1.32±0.06d |
A | 1.23±0.09c | 9.47±0.27d | 1.16±0.07e |
B | 1.19±0.07d | 10.35±0.22c | 1.61±0.11b |
C | 1.57±0.15a | 11.63±0.19a | 1.74±0.09a |
D | 1.35±0.10b | 10.69±0.16b | 1.51±0.08c |
处理 Treatment | Ace指数 Ace index | Chao1指数 Chao1 index | Shannon指数 Shannon index | Simpson指数 Simpson index |
---|---|---|---|---|
CK | 2765.3±172.1bc | 2785.09±162.6b | 6.38±0.42b | 0.0180±0.007a |
A | 2766.9±82.8b | 2769.40±96.9b | 6.53±0.03a | 0.0045±0.001c |
B | 2738.0±154.1d | 2736.34±158.7c | 6.36±0.49b | 0.0100±0.001b |
C | 2830.0±22.4a | 2846.57±42.9a | 6.52±0.04a | 0.0046±0.001c |
D | 2744.6±73.7cb | 2727.34±94.6c | 6.57±0.04a | 0.0040±0.001c |
Tab. 4 Sequence statistics and diversity index under different treatments
处理 Treatment | Ace指数 Ace index | Chao1指数 Chao1 index | Shannon指数 Shannon index | Simpson指数 Simpson index |
---|---|---|---|---|
CK | 2765.3±172.1bc | 2785.09±162.6b | 6.38±0.42b | 0.0180±0.007a |
A | 2766.9±82.8b | 2769.40±96.9b | 6.53±0.03a | 0.0045±0.001c |
B | 2738.0±154.1d | 2736.34±158.7c | 6.36±0.49b | 0.0100±0.001b |
C | 2830.0±22.4a | 2846.57±42.9a | 6.52±0.04a | 0.0046±0.001c |
D | 2744.6±73.7cb | 2727.34±94.6c | 6.57±0.04a | 0.0040±0.001c |
门Phylum | CK | A | B | C | D |
---|---|---|---|---|---|
变形菌门Proteobacteria | 36.82% | 32.84% | 30.23% | 32.58% | 30.26% |
放线菌门Actinobacteria | 27.42% | 25.91% | 27.00% | 27.96% | 26.73% |
绿湾菌门Chloroflex | 9.67% | 14.30% | 15.54% | 12.31% | 15.02% |
酸杆菌门Acidobacteria | 4.15% | 5.08% | 6.41% | 4.66% | 6.27% |
厚壁菌门Firmicutes | 11.05% | 6.02% | 4.74% | 6.79% | 5.65% |
拟杆菌门Bacteroidetes | 3.21% | 4.19% | 4.74% | 4.63% | 4.52% |
芽单胞菌门Gemmatimonadetes | 3.23% | 3.72% | 3.05% | 3.92% | 3.60% |
Saccharibacteria | 0.98% | 3.50% | 2.90% | 2.66% | 2.27% |
Parcubacteria | 0.40% | 0.81% | 1.53% | 1.00% | 1.18% |
Tab. 5 Bacterial community proportion at major phylum under different treatments
门Phylum | CK | A | B | C | D |
---|---|---|---|---|---|
变形菌门Proteobacteria | 36.82% | 32.84% | 30.23% | 32.58% | 30.26% |
放线菌门Actinobacteria | 27.42% | 25.91% | 27.00% | 27.96% | 26.73% |
绿湾菌门Chloroflex | 9.67% | 14.30% | 15.54% | 12.31% | 15.02% |
酸杆菌门Acidobacteria | 4.15% | 5.08% | 6.41% | 4.66% | 6.27% |
厚壁菌门Firmicutes | 11.05% | 6.02% | 4.74% | 6.79% | 5.65% |
拟杆菌门Bacteroidetes | 3.21% | 4.19% | 4.74% | 4.63% | 4.52% |
芽单胞菌门Gemmatimonadetes | 3.23% | 3.72% | 3.05% | 3.92% | 3.60% |
Saccharibacteria | 0.98% | 3.50% | 2.90% | 2.66% | 2.27% |
Parcubacteria | 0.40% | 0.81% | 1.53% | 1.00% | 1.18% |
属Genus | CK | A | B | C | D |
---|---|---|---|---|---|
厌氧绳菌属Anaerolineaceae | 1.68 | 4.50 | 5.66 | 3.38 | 4.11 |
小双孢菌属Microbispora | 2.58 | 3.40 | 6.19 | 3.35 | 2.35 |
嗜酸细菌属Acidobacteria | 2.40 | 2.82 | 4.37 | 2.52 | 4.10 |
Saccharibacteria | 0.98 | 3.50 | 2.90 | 2.66 | 2.27 |
链霉菌属Streptomyces | 2.11 | 2.68 | 2.31 | 2.71 | 2.00 |
芽孢杆菌属Bacillus | 2.17 | 2.13 | 1.78 | 2.70 | 2.23 |
放线菌属Actinomadura | 2.14 | 1.40 | 1.93 | 2.73 | 3.01 |
蔷薇菌属Roseiflexus | 2.10 | 2.30 | 1.80 | 1.94 | 1.60 |
Steroidobacter | 1.17 | 2.01 | 2.21 | 1.96 | 2.01 |
JG30-KF-CM45 | 1.62 | 1.76 | 2.02 | 1.73 | 1.90 |
Tab. 6 Bacterial community proportion at major genus under different treatments %
属Genus | CK | A | B | C | D |
---|---|---|---|---|---|
厌氧绳菌属Anaerolineaceae | 1.68 | 4.50 | 5.66 | 3.38 | 4.11 |
小双孢菌属Microbispora | 2.58 | 3.40 | 6.19 | 3.35 | 2.35 |
嗜酸细菌属Acidobacteria | 2.40 | 2.82 | 4.37 | 2.52 | 4.10 |
Saccharibacteria | 0.98 | 3.50 | 2.90 | 2.66 | 2.27 |
链霉菌属Streptomyces | 2.11 | 2.68 | 2.31 | 2.71 | 2.00 |
芽孢杆菌属Bacillus | 2.17 | 2.13 | 1.78 | 2.70 | 2.23 |
放线菌属Actinomadura | 2.14 | 1.40 | 1.93 | 2.73 | 3.01 |
蔷薇菌属Roseiflexus | 2.10 | 2.30 | 1.80 | 1.94 | 1.60 |
Steroidobacter | 1.17 | 2.01 | 2.21 | 1.96 | 2.01 |
JG30-KF-CM45 | 1.62 | 1.76 | 2.02 | 1.73 | 1.90 |
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