Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (4): 730-736.DOI: 10.3969/j.issn.1000-2561.2020.04.014

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Clonging and Expression Analysis of WRKY52 Gene in Dimocarpus longan

XUE Xin1,2,3,ZHOU Xinzhi4,JUE Dengwei3,*()   

  1. 1. Lingnan Normal University, Zhanjiang, Guangdong 524048, China
    2. College of Horticulture, Hainan University, Haikou, Hainan 570228, China
    3. South Subtropical Corp Research Institute, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Zhanjiang, Guangdong 524091, China
    4. Chongqing Academy of Agricultural Sciences, Changqing 401329, China
  • Received:2019-07-08 Revised:2019-08-20 Online:2020-04-25 Published:2020-05-09
  • Contact: JUE Dengwei

Abstract:

WRKY transcription factors plays an important role in plant growth, development and stress response. Based on a previous study, we found several WRKY genes, such as DlWRKY52, were participated in the process of floral induction and stress response. To further reveal the function of longan WRKY genes, DlWRKY52 was cloned using the leave cDNA of ‘Sijimi’ longan as the template. Meanwhile, the sequence characteristics, tissue expression patterns, flower and fruit development process expression patterns and subcellular localization were also studied. Bioinformatics analysis indicated that the complete open reading frame (ORF) box of DlWRKY52 was 918 bp, encoding 305 amino acid residues. The amino acid sequence alignment analysis showed that DlWRKY52 contained a typical WRKY domain and a zinc finger structure, belonging to Group Ⅱc. The result of qRT-PCR showed that DlWRKY52 was highly expressed in leave, stem and fruit organs, significantly up-regualted in the pulp 80 days post-anthesis. The transient expression of Arabidopsis protoplasts demonstrated that DlWRKY52 protein was localized to the nucleus, indicating that DlWRKY52, as a typical transcription factor, is localized to the nucleus, and might participate in the regulating of longan floral induction and early fruit development.

Key words: Dimocarpus longan, WRKY, transcription factor, expression analysis, protein subcellular localization

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