Cloning, Bioinformatics and Expression Analysis of Mango <i>MiCOL6</i> Gene
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (4): 715-721.DOI: 10.3969/j.issn.1000-2561.2020.04.012

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Cloning, Bioinformatics and Expression Analysis of Mango MiCOL6 Gene

LU Xinxi,LUO Cong,ZHANG Xiujuan,YU Haixia,LIU Yuan,HE Xinhua()   

  1. College of Agriculture, Guangxi University / State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, Guangxi University, Nanning, Guangxi 530004, China
  • Received:2019-04-29 Revised:2019-09-09 Online:2020-04-25 Published:2020-05-09
  • Contact: HE Xinhua

Abstract:

CONSTANS (CO) is a core regulator of the photoperiod pathway, which integrates light quality and clock output signals to regulate the floral transformation in plants. A full-length cDNA of MiCOL6 gene was obtained from transcriptome data analysis and then verified by RT-PCR amplification in mango. Sequences analysis showed that the open reading frame was 678 bp in length, coding 226 amino, the molecular weight and isoelectric point (pI) of MiCOL6 was 26.88 kDa and 4.85, respectively; and MiCOL6 was a non-secreted hydrophilic protein. Conserved domain and phylogenetic tree analysis showed that MiCOL6 contained only one CCT domain and belonged to the group IV of the CO gene family. The secondary structure analysis showed that the protein mainly consisted of random curl and α-helix. The subcellular localization prediction showed that MiCOL6 was likely to be localized in the cytoplasm membrane. Expression pattern analysis showed that the MiCOL6 was the highest expressed in leaves during the bud differentiation period. The results of the experiment would provide a theoretical basis for the further study on the function of MiCOL6 in mango.

Key words: mango, CONSTANS, MiCOL6, gene cloning, bioinformatics analysis, expression pattern analysis

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