Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (4): 715-721.DOI: 10.3969/j.issn.1000-2561.2020.04.012
• Biotechnology and Tissue Culture • Previous Articles Next Articles
LU Xinxi,LUO Cong,ZHANG Xiujuan,YU Haixia,LIU Yuan,HE Xinhua()
Received:
2019-04-29
Revised:
2019-09-09
Online:
2020-04-25
Published:
2020-05-09
Contact:
HE Xinhua
CLC Number:
LU Xinxi,LUO Cong,ZHANG Xiujuan,YU Haixia,LIU Yuan,HE Xinhua. Cloning, Bioinformatics and Expression Analysis of Mango MiCOL6 Gene[J]. Chinese Journal of Tropical Crops, 2020, 41(4): 715-721.
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引物名称 Primers ID | 引物序列(5°-3°) Primers sequence (5°-3°) |
---|---|
MiCOL6u | AAATGGGGTACCATTCATTGT |
MiCOL6d | CTAAGCTTCTCAGGAAACTCT |
qCOL6u | CTTATGGTGAAGAGGCCATGA |
qCOL6d | CCAGTCCATGAAATCCCAAC |
qActin1u | CCGAGACATGAAGGAGAAGC |
qActin1d | GTGGTCTCATGGATACGAGCA |
Tab. 1 Primers sequence
引物名称 Primers ID | 引物序列(5°-3°) Primers sequence (5°-3°) |
---|---|
MiCOL6u | AAATGGGGTACCATTCATTGT |
MiCOL6d | CTAAGCTTCTCAGGAAACTCT |
qCOL6u | CTTATGGTGAAGAGGCCATGA |
qCOL6d | CCAGTCCATGAAATCCCAAC |
qActin1u | CCGAGACATGAAGGAGAAGC |
qActin1d | GTGGTCTCATGGATACGAGCA |
Fig. 2 Phylogenetic tree of CO proteins The GenBank accession numbers of the amino acids encoding each gene are as follows: Arabidopsis thaliana AtCOL (NM_121589), AtCOL1 (NM_ 121589), AtCOL2 (NM_111105), AtCOL6 (NM_ 106047), AtCOL7 (NM_103803), AtCOL9 (AB023039), AtCOL10 (NM_117613), AtCOL11 (NM_ 113084), AtCOL12 (NM_130356), AtCOL16 (NM_ 102355); Populus trichocarpa PtCOL16 (XP_024449388), Theobroma cacao TaCOL8 (XP_007016977).
Fig. 3 Multiple alignments of MiCOL6 with other species The GenBank accession numbers of the amino acids encoding each gene are as follows: Populus trichocarpa PtCOL16 (XP_024449388), Quercus suber QsCOL7 (XP_023874898), Gossypium arboretum GaCOL16 (XP_017641001).
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