Effects of Two Polyamines on in Vitro Flowering of Dendrobium officinale Kimura et Migo

doi:10.3969/j.issn.1000-2561.2020.04.011

Abstract

Abstract:

The effects of different concentrations of exogenous putrescine (Put) and spermine (Spm) on the flowering of Dendrobium officinale Kimura et Migo were studied using the rootless plantlets in vitro. The flowering rate of D. officinale could be increased by adding appropriate amount of Put and Spm in the medium. The flowering rate of D. officinale was the highest (30.47%) when the concentration of Put was 0.4 mg/L, and the flowering rate of D. officinale was 22.26% when the concentration of Spm was 0.2 mg/L. The time to have the first flower bud appeared of D. officinale was the shortest (83.33 d) when the concentration of Put was 0.2 mg/L, and the viewing period of D. officinale could be prolonged by 43.33 days. When the concentration of Put was 0.4 mg/L, the content of soluble sugar and soluble protein was the highest. The content of total N was the highest in CK. When the concentration of Spm was 0.6 mg/L, the C/N ratio of the plant was the highest. The concentration of Put 0.4 mg/L would be beneficial to the accumulation of carbon and nitrogen compounds, which could promote the flowering rate of D. officinale.

Key words: Dendrobium officinale Kimura et Migo, polyamines, in vitro flowering, rate of flowering

CLC Number:

S682.31

LI Ru,LI Zhilin,BAI Jiankun,SHANG Zhengrui,ZHAO Liying,WANG Yuying. Effects of Two Polyamines on in Vitro Flowering of Dendrobium officinale Kimura et Migo[J]. Chinese Journal of Tropical Crops, 2020, 41(4): 709-714.

Figures/Tables 6

References 28

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处理编号 Treatment number	开花率 Flowering percentage/%	始花期 Day to first flower bud/d	花期 Blooming period/d	花朵大小 Flower size/cm	花梗长 Peduncle length/cm
P1	11.52±0.11^e	83.33±3.06^b	43.33±2.52^a	1.33±0.06^b	1.07±0.06^a
P2	30.47±1.66^a	95.67±13.05^a	24.33±9.29^b	1.33±0.81^b	0.64±0.36^b
P3	16.47±0.21^c	98.33±3.06^a	30.05±2.16^b	2.43±0.06^a	0.62±0.07^b
S1	22.26±0.61^b	105.67±1.53^a	27.67±1.53^b	1.47±0.06^b	0.80±0.15^ab
S2	17.54±0.54^d	93.42±1.35^a	24.13±1.17^b	1.34±0.51^b	0.61±0.14^b
S3	14.23±0.45^d	84.33±1.53^b	28.67±1.53^b	1.15±0.82^b	0.53±0.15^b
CK	0±0^f	0±0^c	0±0^c	0±0^c	0±0^c

处理编号 Treatment number	开花率 Flowering percentage/%	始花期 Day to first flower bud/d	花期 Blooming period/d	花朵大小 Flower size/cm	花梗长 Peduncle length/cm
P1	11.52±0.11^e	83.33±3.06^b	43.33±2.52^a	1.33±0.06^b	1.07±0.06^a
P2	30.47±1.66^a	95.67±13.05^a	24.33±9.29^b	1.33±0.81^b	0.64±0.36^b
P3	16.47±0.21^c	98.33±3.06^a	30.05±2.16^b	2.43±0.06^a	0.62±0.07^b
S1	22.26±0.61^b	105.67±1.53^a	27.67±1.53^b	1.47±0.06^b	0.80±0.15^ab
S2	17.54±0.54^d	93.42±1.35^a	24.13±1.17^b	1.34±0.51^b	0.61±0.14^b
S3	14.23±0.45^d	84.33±1.53^b	28.67±1.53^b	1.15±0.82^b	0.53±0.15^b
CK	0±0^f	0±0^c	0±0^c	0±0^c	0±0^c

处理 Treatment	叶片数 Number of leaves plant	叶长 Leaf length/ cm	叶宽 Leaf width/ mm	根数（条/株） Number of roots /(root·plant^-1)	根粗 Root diameter/ mm	根长 Root length/mm	株高 Plant height/cm	鲜质重 Fresh weights/g
P1	4.33±0.58^b	1.12±0.28^b	6.24±0.85^c	8.00±1.00^bc	1.37±0.21^ab	6.37±0.91^bc	2.40±0.23^c	0.40±0.01^c
P2	5.00±1.00b	1.57±0.14^ab	6.53±1.57^bc	5.00±1.73^c	1.21±0.07^b	4.21±0.99^de	2.31±0.25^c	0.33±0.13^c
P3	4.33±1.15b	1.46±0.43^ab	6.54±1.26^bc	7.67±2.31^bc	1.31±0.08^ab	4.64±1.56^cd	2.80±0.66^c	0.39±0.11^c
S1	6.33±0.58^a	2.72±0.56^a	9.33±1.17^ab	13.00±7.81^ab	1.65±0.33^a	2.71±0.61^e	4.90±0.51^b	1.06±0.31^b
S2	7.33±0.58^a	2.17±0.29^a	9.64±2.56^a	18.67±4.93^a	1.56±0.16^ab	9.62±0.81^a	6.67±1.15^a	1.45±0.34^a
S3	4.33±0.58^b	1.57±0.52^ab	7.37±2.31^abc	8.00±2.00^bc	1.42±0.22^ab	6.59±0.78^b	2.83±0.57^c	0.63±0.05^c
CK	4.00±0^b	0.93±0.16^b	4.97±0.32^c	4.67±0.58^c	1.41±0.26^ab	4.57±0.99^cd	2.27±0.29^c	0.37±0.12^c

处理 Treatment	叶片数 Number of leaves plant	叶长 Leaf length/ cm	叶宽 Leaf width/ mm	根数（条/株） Number of roots /(root·plant^-1)	根粗 Root diameter/ mm	根长 Root length/mm	株高 Plant height/cm	鲜质重 Fresh weights/g
P1	4.33±0.58^b	1.12±0.28^b	6.24±0.85^c	8.00±1.00^bc	1.37±0.21^ab	6.37±0.91^bc	2.40±0.23^c	0.40±0.01^c
P2	5.00±1.00b	1.57±0.14^ab	6.53±1.57^bc	5.00±1.73^c	1.21±0.07^b	4.21±0.99^de	2.31±0.25^c	0.33±0.13^c
P3	4.33±1.15b	1.46±0.43^ab	6.54±1.26^bc	7.67±2.31^bc	1.31±0.08^ab	4.64±1.56^cd	2.80±0.66^c	0.39±0.11^c
S1	6.33±0.58^a	2.72±0.56^a	9.33±1.17^ab	13.00±7.81^ab	1.65±0.33^a	2.71±0.61^e	4.90±0.51^b	1.06±0.31^b
S2	7.33±0.58^a	2.17±0.29^a	9.64±2.56^a	18.67±4.93^a	1.56±0.16^ab	9.62±0.81^a	6.67±1.15^a	1.45±0.34^a
S3	4.33±0.58^b	1.57±0.52^ab	7.37±2.31^abc	8.00±2.00^bc	1.42±0.22^ab	6.59±0.78^b	2.83±0.57^c	0.63±0.05^c
CK	4.00±0^b	0.93±0.16^b	4.97±0.32^c	4.67±0.58^c	1.41±0.26^ab	4.57±0.99^cd	2.27±0.29^c	0.37±0.12^c

碳氮化合物 Carbon and nitrogen compounds	花朵大小 Flower size	始花期 Days to first flower bud	花梗长 Peduncle length	花期 Blooming period	开花率 Flowering percentage
C/N	0.245	0.281	0.279	0.429	0.110
全N含量	-0.014	-0.057	0.099	0.058	0.011
蛋白质含量	0.140	0.138	0.220	0.300	0.062
可溶性糖含量	0.250	0.506^*	0.512^*	0.457^*	0.541^*