Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (4): 685-693.DOI: 10.3969/j.issn.1000-2561.2020.04.008

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Analysis of Physio-biochemical Characteristics of T2 α-tubulin SoTUA Transgenic Sugarcane

CHEN Jiaoyun1,KHAN Qaisar1,WEI Jianglu1,TANG Lihua1,DONG Dengfeng1,*(),LI Yangrui1,2,*()   

  1. 1. College of Agriculture, Guangxi University, Nanning, Guangxi 530004, China
    2. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning, Guangxi 530007, China
  • Received:2019-06-26 Revised:2019-08-22 Online:2020-04-25 Published:2020-05-09
  • Contact: DONG Dengfeng,LI Yangrui


Six SoTUA transgenic lines (T1, T2, T3, T4, T5, T6) were selected from the positive SoTUA transgenic sugarcane lines of T2 generation after PCR amplification and sequencing. The transgenic lines and control (wild type, WT) were treated with low temperature stress (4 ℃), and the level of malondialdehyde (MDA) and osmotic adjustment substances (soluble sugar, soluble protein) and activities of peroxidase (POD) and superoxide dismutase SOD were determined, and RT-qPCR analysis was done for SoTUA gene expression in sugarcane leaves at 0, 5 and 10 days of the treatment. The relative expression of α-tubulin transcription factor in the SoTUA transgenic sugarcane and WT was up-regulated, getting higher with the cold stress time under low temperature treatment at 4 ℃. The relative expression level was always higher in the SoTUA transgenic lines than that in WT at 5 and 10 days of the treatment. At the 10th day, the relative expression level of the transgenic line T1 was significantly higher than that of other transgenic strains (P<0.05). The content of MDA increased gradually under cold stress, but it was found significantly lower in the transgenic sugarcane than that in WT by 41.77% (P<0.05). The trend of POD activity varied among different lines, but it was higher in the SoTUA transgenic lines than in WT (P<0.05). The SOD activity of transgenic lines T1, T2, T4 and T6 increased first and then decreased while SOD activity of WT and transgenic lines T3 and T5 increased gradually. The soluble sugar content increased gradually and the trend of soluble protein varied in different strains. The expression of SoTUA gene was positively correlated with soluble sugar. Using the membership function comprehensive analysis method, the ranking result for cold resistance was obtained as T3 > T2 > T1 > T5 > T6 > T4 > WT. Up-regulated expression of SoTUA gene in the transgenic sugarcane could improve cold resistance, and the genetic stability is good.

Key words: sugarcane, SoTUA gene, cold resistance, physio-biochemical characteristic, genetic stability

CLC Number: