Transplantation Effects of Different Nursery Substrates on the Growth of Tissue Culture Seedlings of Mytilaria laosensis

doi:10.3969/j.issn.1000-2561.2020.04.004

Abstract

Abstract:

The effect of different nursery substrates on the transplant survival rate, growth indicator and seedling quality index of M. laosensis tissue culture seedlings was investigated by setting five different nursery substrates according to the volume ratio of two nursery substrates (yellow soil and peat soil). T₁(100% yellow soil) and T₃ (60% yellow soil+40% peat soil) were more conducive to the survival of M. laosensis seedlings after transplantation, and the survival rate of the seedlings transplanted for 1 month was the highest (83.33% for T₁, and 81.11% for T₃). Plant height, leaf number and ground diameter of M. laosensis seedlings increased first and then decreased with the increase of the proportion of peat soil, and reached the maximum at T_3.The three indicators of the seedlings transplanted after 6 months for T₃ were 30.93 cm, 8.1 and 5.04 mm, respectively, which were significantly higher than those of the other four treatments (P<0.01). The aboveground and underground dry weight of M. laosensis seedlings transplanted after 6 months were T₃ > T₄ > T₅ > T₂ > T₁, and the aboveground and underground dry weight for T₃ were 8.51 and 5.56 times of those of T_1.Furthermore, the seedling quality index of T₃ reached 1.13, which was significantly higher than that of T₅, T₂ and T₁ (P<0.05). Therefore, the mixture nursery substrates with 60% yellow soil and 40% peat soil were the ideal nursery substrates for tissue culture seedlings of M. laosensis.

Key words: Mytilaria laosensis, tissue culture seedlings, nursery substrates, effect of transplantation, seedling quality index

CLC Number:

S722.3+7

LIU Zhenxiang,LI Rongsheng,ZOU Wentao,QIU Zhenfei,YU Niu,YANG Jinchang. Transplantation Effects of Different Nursery Substrates on the Growth of Tissue Culture Seedlings of Mytilaria laosensis[J]. Chinese Journal of Tropical Crops, 2020, 41(4): 655-660.

Figures/Tables 7

References 27

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处理 Treatment	容重 Volume weight /(g·cm^-3)	总孔隙 Total porosity/%	持水力 Water retention ability/%
T₁	1.18	44.37	35.60
T₂	1.05	49.34	43.76
T₃	0.88	54.61	56.15
T₄	0.69	54.25	69.71
T₅	0.46	55.98	88.22

处理 Treatment	容重 Volume weight /(g·cm^-3)	总孔隙 Total porosity/%	持水力 Water retention ability/%
T₁	1.18	44.37	35.60
T₂	1.05	49.34	43.76
T₃	0.88	54.61	56.15
T₄	0.69	54.25	69.71
T₅	0.46	55.98	88.22

处理 Treatment	pH	有机质 Organic matter/ (g·kg^-1)	全氮 Total nitrogen/ (g·kg^-1)	全磷 Total phosphorus/ (g·kg^-1)	全钾 Total potassium/ (g·kg^-1)	碱解氮Available nitrogen/ (mg·kg^-1)	有效磷Available phosphorus/ (mg·kg^-1)	速效钾Available potassium/ (mg·kg^-1)
T₁	5.31	1.92	0.11	0.08	23.77	7.50	0.25	100.40
T₂	5.43	23.28	0.46	0.12	23.39	38.02	24.50	432.86
T₃	5.60	55.00	0.99	0.18	22.86	83.35	60.48	925.82
T₄	5.85	106.90	1.76	0.31	21.94	157.55	119.36	1732.99
T₅	6.35	207.34	3.36	0.51	20.18	301.06	233.36	3294.92

处理 Treatment	pH	有机质 Organic matter/ (g·kg^-1)	全氮 Total nitrogen/ (g·kg^-1)	全磷 Total phosphorus/ (g·kg^-1)	全钾 Total potassium/ (g·kg^-1)	碱解氮Available nitrogen/ (mg·kg^-1)	有效磷Available phosphorus/ (mg·kg^-1)	速效钾Available potassium/ (mg·kg^-1)
T₁	5.31	1.92	0.11	0.08	23.77	7.50	0.25	100.40
T₂	5.43	23.28	0.46	0.12	23.39	38.02	24.50	432.86
T₃	5.60	55.00	0.99	0.18	22.86	83.35	60.48	925.82
T₄	5.85	106.90	1.76	0.31	21.94	157.55	119.36	1732.99
T₅	6.35	207.34	3.36	0.51	20.18	301.06	233.36	3294.92

处理 Treatment	0.5个月0.5 months		1个月1 month		6个月6 months
处理 Treatment	株高 Plant height/cm	叶片数 Number of leaves	株高 Plant height/cm	叶片数 Number of leaves	株高 Plant height/cm	叶片数 Number of leaves	地径 Ground diameter/mm
T₁	3.26±0.08^bB	2.97±0.13^aA	5.08±0.11^cC	3.34±0.10^bB	14.63±0.61^eD	4.47±0.47^bC	3.01±0.09^dC
T₂	3.80±0.10^aA	2.85±0.11^abA	5.82±0.21^bB	3.46±0.13^bB	19.03±0.84^dC	4.77±0.39^bC	3.57±0.16^cBC
T₃	3.51±0.10^bAB	2.92±0.11^abA	6.42±0.22^aA	3.97±0.12^aA	30.93±0.75^aA	8.13±0.59^aA	5.04±0.25^aA
T₄	3.48±0.09^bAB	2.91±0.11^abA	5.09±0.14^cC	3.36±0.11^bB	26.17±0.94^bB	7.33±0.33^aAB	4.25±0.13^bB
T₅	3.34±0.10^bB	2.58±0.12^bA	5.06±0.12^cC	3.21±0.08^bB	21.57±0.55^cC	5.73±0.27^bBC	3.79±0.07^bcB