Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (3): 609-614.DOI: 10.3969/j.issn.1000-2561.2020.03.026
• Ecology • Previous Articles Next Articles
LIU Zifan,LIU Peipei,YAN Wenjing,MA Xiaowei
Received:
2019-05-14
Revised:
2019-08-26
Online:
2020-03-25
Published:
2020-04-16
CLC Number:
LIU Zifan,LIU Peipei,YAN Wenjing,MA Xiaowei. Effects of Rubber-cassava Intercropping on Soil Fungal Community Structure in Rhizosphere of Rubber Trees[J]. Chinese Journal of Tropical Crops, 2020, 41(3): 609-614.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.rdzwxb.com/EN/10.3969/j.issn.1000-2561.2020.03.026
种植方式 Cultivation methods | pH | 碱解氮 Alkalide nitrogen / (mg∙kg-1) | 速效磷 Available phosphorus / (mg∙kg-1) | 速效钾 Available potassium / (mg∙kg-1) | 有机质 Organic matter /(g∙kg-1) |
---|---|---|---|---|---|
单作 | 5.05±0.02 | 271.60±6.94* | 34.64±0.13* | 40.75±0.31* | 11.65±0.33* |
间作 | 4.92±0.03 | 192.64±10.22 | 11.58±1.18 | 34.88±0.17 | 6.71±0.50 |
Tab. 1 Soil essential physiochemical properties in rhizosphere of rubber trees
种植方式 Cultivation methods | pH | 碱解氮 Alkalide nitrogen / (mg∙kg-1) | 速效磷 Available phosphorus / (mg∙kg-1) | 速效钾 Available potassium / (mg∙kg-1) | 有机质 Organic matter /(g∙kg-1) |
---|---|---|---|---|---|
单作 | 5.05±0.02 | 271.60±6.94* | 34.64±0.13* | 40.75±0.31* | 11.65±0.33* |
间作 | 4.92±0.03 | 192.64±10.22 | 11.58±1.18 | 34.88±0.17 | 6.71±0.50 |
种植方式 Cultivation methods | 原始序列数 Number of origin sequence | 有效序列数 Number of available sequence | 平均序列长度 Average sequence length /bp | 分类单元 OTU | 样品覆盖度 Sample coverage |
---|---|---|---|---|---|
单作 | 91 027 | 87 609 | 352.27 | 1 010 | 0.996 |
间作 | 83 779 | 73 883 | 347.86 | 1 132 | 0.994 |
Tab. 2 Sequencing quality of soil fungal community in rhizosphere of rubber trees
种植方式 Cultivation methods | 原始序列数 Number of origin sequence | 有效序列数 Number of available sequence | 平均序列长度 Average sequence length /bp | 分类单元 OTU | 样品覆盖度 Sample coverage |
---|---|---|---|---|---|
单作 | 91 027 | 87 609 | 352.27 | 1 010 | 0.996 |
间作 | 83 779 | 73 883 | 347.86 | 1 132 | 0.994 |
种植方式 Cultivation methods | Chao1指数 Chao1 index | Shannon指数 Shannon index | Simpson指数 Simpson index |
---|---|---|---|
单作 | 1528.1 | 3.52* | 0.109 |
间作 | 1330.4 | 3.05 | 0.189* |
Tab. 3 Alpha diversity index of soil fungi in rhizosphere of rubber trees
种植方式 Cultivation methods | Chao1指数 Chao1 index | Shannon指数 Shannon index | Simpson指数 Simpson index |
---|---|---|---|
单作 | 1528.1 | 3.52* | 0.109 |
间作 | 1330.4 | 3.05 | 0.189* |
项目 Items | pH | 碱解氮 Alkalide nitrogen | 速效磷 Available phosphorus | 速效钾 Available potassium | 有机质 Organic matter |
---|---|---|---|---|---|
OTU | 0.221 | -0.809** | -0.811** | -0.646* | -0.019 |
Chao1 index | -0.156 | -0.884** | -0.890** | -0.746** | -0.037 |
Shannon index | 0.204 | -0.639* | -0.734** | -0.281 | 0.168 |
Simpson index | 0.186 | 0.538 | 0.656* | 0.087 | -0.31 |
Tab. 4 Correlation between fungal alpha diversity and rhizosphere soil physiochemical properties
项目 Items | pH | 碱解氮 Alkalide nitrogen | 速效磷 Available phosphorus | 速效钾 Available potassium | 有机质 Organic matter |
---|---|---|---|---|---|
OTU | 0.221 | -0.809** | -0.811** | -0.646* | -0.019 |
Chao1 index | -0.156 | -0.884** | -0.890** | -0.746** | -0.037 |
Shannon index | 0.204 | -0.639* | -0.734** | -0.281 | 0.168 |
Simpson index | 0.186 | 0.538 | 0.656* | 0.087 | -0.31 |
项目 Items | pH | 碱解氮 Alkalide nitrogen | 速效磷 Available phosphorus | 速效钾 Available potassium | 有机质 Organic matter |
---|---|---|---|---|---|
Ascomycota | 0.211 | 0.216 | 0.332 | -0.267 | -0.649* |
Basidiomycota | 0.167 | -0.406 | -0.529 | 0.13 | 0.407 |
Zygomycota | 0.288 | -0.125 | -0.222 | 0.134 | 0.315 |
Chytridiomycota | 0.199 | -0.371 | -0.44 | -0.122 | 0.295 |
Glomeromycota | 0.259 | -0.586* | -0.657* | -0.324 | -0.058 |
Tab. 5 Correlation between the proportion of dominate fungal phyla at phylum level and rhizosphere soil properties
项目 Items | pH | 碱解氮 Alkalide nitrogen | 速效磷 Available phosphorus | 速效钾 Available potassium | 有机质 Organic matter |
---|---|---|---|---|---|
Ascomycota | 0.211 | 0.216 | 0.332 | -0.267 | -0.649* |
Basidiomycota | 0.167 | -0.406 | -0.529 | 0.13 | 0.407 |
Zygomycota | 0.288 | -0.125 | -0.222 | 0.134 | 0.315 |
Chytridiomycota | 0.199 | -0.371 | -0.44 | -0.122 | 0.295 |
Glomeromycota | 0.259 | -0.586* | -0.657* | -0.324 | -0.058 |
[1] | 郑定华, 袁淑娜, 陈俊明 , 等. 橡胶林下间作广金钱草的产量及药材质量研究[J]. 中药材, 2017,40(12):2765-2771. |
[2] | 刘敏, 张广宇, 张永北 , 等. 竹荪间作对橡胶园土壤微生物区系与群落功能多样性的影响[J]. 江苏农业科学, 2018,46(13):295-298. |
[3] | 黄洁, 周建国, 林世欣 , 等. 对胶园间作木薯的思考及展望[J]. 江西农业学报, 2014,26(1):64-68, 71. |
[4] | 林位夫, 曾宪海, 谢贵水 , 等. 关于橡胶园间作的思考与实践[J]. 中国热带农业, 2011(4):11-15. |
[5] | Larsonde R A. Introduction to floriculture[M]. New York: Academic Press, 1980. |
[6] | 蔡吉苗, 陈瑶, 潘羡心 , 等. 海南橡胶树棒孢霉落叶病病情调查与病原鉴定[J]. 热带农业科学, 2008,28(5):1-7, 10. |
[7] | 杨国峰 . 加蓬的胶粮间作系统[J]. 世界热带农业信息, 2000(6):1-2. |
[8] | 方敏, 徐小蓉, 唐明 , 等. 马缨杜鹃根系微生物群落结构及其变化[J]. 微生物学报, 2019,59(8):1522-1534. |
[9] | 孙启武, 毛琪 . 浅析微生物对土壤肥力的贡献[J]. 农技服务, 2015,32(9):96. |
[10] | Yang H W, Li J, Xiao Y H , et al. An integrated insight into the relationship between soil microbial community and tobacco bacterial wilt disease[J]. Frontiers in Microbiology, 2017,8:2179. |
[11] | 刘培培, 刘子凡, 刘乔颖 , 等. 木薯器官及其混合物水浸提液对橡胶树棒孢霉落叶病病菌的化感作用[J]. 热带作物学报, 2018,39(2):338-342. |
[12] | 闫文静, 刘子凡, 张婷婷 , 等. 木薯器官及其腐解物水浸液对橡胶树白根病病菌的化感作用[J]. 热带作物学报, 2019,40(1):123-129. |
[13] | 史芳芳, 李向泉 . 葡萄根际土壤真菌群落多样性分析[J]. 中国农业科技导报, 2019,21(7):47-58. |
[14] | Agrios G N. Plant Pathology[M]. 5th ed. US: Academic Press, 2005. |
[15] | 鲍士旦 . 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2008. |
[16] | 高东, 何霞红 . 生物多样性与生态系统稳定性研究进展[J]. 生态学杂志, 2010,29(12):2507-2513. |
[17] | 扈进冬, 吴远征, 魏艳丽 , 等. 木霉拌种剂对小麦根际土壤真菌群落多样性的影响[J]. 山东科学, 2019,32(1):46-51. |
[18] | Deacon J. Introduction: the fungi and fungal activities[M]//Deacon J. Fungal Biology. Malden, MA: Blackwell Publishing Ltd, 2013: 1-15. |
[19] | 李桥 . 基于高通量测序技术下土壤微生物群落结构的研究[D]. 济南: 山东师范大学, 2014. |
[20] | 李旭锐, 张燕, 张杰 , 等. 苹果园不同间作模式对土壤理化性质及微生物群落的PLFA分析[J]. 北京农学院学报, 2019,34(3):30-36. |
[21] | Concha Meyer A, Eifert J D, Williams R C , et al. Shelf life determination of fresh blueberries (Vaccinium corymbosum) stored under controlled atmosphere and ozone[J]. International Journal of Food Science, 2015,2015:164143. |
[22] | Berendsen R L, Pieterse C, Bakker P . The rhizosphere microbiome and plant health[J]. Trends in Plant Science, 2012,17(8):478-486. |
[23] | Ahonsi M O, Agindotan B O, Gray M E , et al. First report of basal stem rot and foliar blight caused by Pythium sylvaticum on Miscanthus sinensis in Illinois[J]. Plant Disease, 2011,95(5):616. |
[24] | 徐江兵, 林先贵, 王一明 , 等. 施用茶树菇栽培废料对青菜土壤中微生物学特征的影响[J]. 农业环境科学学报, 2012,31(1):131-136. |
[25] | 秦立金, 于田田, 王佳明 , 等. 黄瓜与西芹间作对黄瓜土壤真菌 ITS 多样性分析[J]. 中国生态农业学报(中英文), 2019,27(4):529-536. |
[26] | Yelle D J, Ralph J, Lu F C , et al. Evidence for cleavage of lignin by a brown rot basidiomycete[J]. Environmental Microbiology, 2008,10(7):1844-1849. |
[27] | 张敏, 周鹏, 季永华 . 苦楝一小麦农林复合生态系统土壤真菌群落结构分析[J]. 林业科学, 2015,51(10):26-34. |
[28] | 于莉, 鲍文杰, 张英 , 等. Phoma和Phyllosticta两属分类的研究进展[J]. 吉林农业大学学报, 1995,17(4):102-107. |
[29] | 崔云凤, 黄云, 蒋伶活 . 农业生产上几种重要的赤霉属真菌研究进展[J]. 中国农学通报, 2007,23(7):441-446. |
[30] | 周小露, 宋丽莎, 王利荣 , 等. 黔南州茶园土壤中优势菌株烟曲霉的分离·纯化及鉴定[J]. 安徽农业科学, 2018,46(22):115-118. |
[31] | Vargas G S, Meriles J, Conforto C , et al. Response of soil microbial communities to different management practices in surface soils of a soybean agroecosystem in Argentina[J]. European Journal of Soil Biology, 2011,47(1):55-60. |
[32] | Güsewell S, Gessner M O . N:P ratios influence litter decomposition and colonization by fungi and bacteria in microcosms[J]. Functional Ecology, 2009,23(1):211-219. |
[1] | NONG Zemei,SHI Guoying,ZENG Quan,YE Xuelian,QIN Huadong,HU Chunjin. Analysis on Enzyme Activity and Microbial Community Diversity in Rhizosphere Soil of Different Sugarcane Varieties [J]. Chinese Journal of Tropical Crops, 2020, 41(4): 819-828. |
[2] | ZHANG Chuanjin,REN Kuiyu,GUO Shuang,PANG Shichan,WANG Shuaishuai,YANG Shangdong. Soil Biological Properties in Rhizosphere of Pea (Pisum sativum L.) Improved by Magnetic Treatments [J]. Chinese Journal of Tropical Crops, 2020, 41(4): 829-836. |
[3] | YE Wenyu,XIE Xuze,XU Yuying,NI Mingyue,HU Hongli,YU Wenying,Norvienyeku Justice,LU Guodong. High-throughput Sequencing Analysis of Fungal Community in Rhizosphere Soils of Two Plants for Mushroon Cultivation (JUNCAO) [J]. Chinese Journal of Tropical Crops, 2020, 41(3): 556-563. |
[4] | HUANG Xuguang,QIN Ling,HUANG Lidan,LU Yansong,LUO Enbo,HUANG Lingpu,LIU Yuefei. Variation Characteristics of Drought and Rehydration on the Growth of Hibiscus rosa-sinensis Linn. and Soil Microbial Diversity in Rhizosphere [J]. Chinese Journal of Tropical Crops, 2020, 41(2): 401-408. |
[5] | YE Wenyu,LIAO Haiping,XU Yuying,XIE Xuze,NI Mingyue,HU Hongli,YU Wenying,LU Guodong. Rhizosphere Soil Bacterial Community of Juncao by High-throughput Sequencing Techniques [J]. Chinese Journal of Tropical Crops, 2019, 40(9): 1783-1788. |
[6] | DU Yinggang,JI Qinge,LAI Zhongxiong. Preliminary Analysis of Transcriptome and Olfaction-related Genes in Bactrocera tau (Walker) [J]. Chinese Journal of Tropical Crops, 2019, 40(9): 1796-1803. |
[7] | WANG Haibin,CHEN Xiaoting,ZHAO Hu,WANG Yuhua,ZHANG Qingxu,WANG Peng,YE Jianghua,DING Li. The Autotoxicity of Tea Tree Rhizosphere Soil Chemicals and the Effect of Soil Microbial Diversity [J]. Chinese Journal of Tropical Crops, 2019, 40(9): 1847-1857. |
[8] | LI Kejie,LUO Bi,TANG Xiaomin,ZHOU Liangyun,YANG Quan,PAN Liming. Changes of Physicochemical Properties and Available Elements in Rhizosphere Soil under Different Intercropping Patterns of Citrus medica L. var. sarcodactylis Swingle [J]. Chinese Journal of Tropical Crops, 2019, 40(9): 1865-1872. |
[9] | LIU Shanting,LUO Xinglu,WU Meiyan,TANG Zhiping,WANG Chaochao,ZHANG Jialing. Comparison of Cassava Yield and Soil Microbial Characteristics under Continuous Cropping and Rotation [J]. Chinese Journal of Tropical Crops, 2019, 40(8): 1468-1473. |
[10] | ZHANG Chuanjin,WU Renmin,WANG Shuaishuai,ZHOU Liuqiang,YANG Shangdong,TAN Hongwei. Change of Root Growth of Muskmelon,Soil Biological Properties and Bacterial Diversity in Rhizosphere under Wood Vinegar Application [J]. Chinese Journal of Tropical Crops, 2019, 40(7): 1265-1271. |
[11] | WANG Haibin,CHEN Xiaoting,WANG Yuhua,ZHAO Hu,ZHANG Huabin,DING Li,KONG Xianghai,YE Jianghua. Effects of Rhizosphere Soil Chemicals on Growth and Quality of Tea Trees at Different Ages [J]. Chinese Journal of Tropical Crops, 2019, 40(11): 2149-2159. |
[12] | LIN Yan, HE Zidi, MAO Jipeng, JIANG Kaibin, WANG Jinbang, HUANG Shaowei. Development and Preliminary Analysis of SSR Locus in Schima superba Genome [J]. Chinese Journal of Tropical Crops, 2018, 39(9): 1766-1771. |
[13] | WANG Haibin, CHEN Xiaoting, DING Li, QIU Fengyan, KONG Xianghai, YE Jianghua , JIA Xiaoli. Analysis on Autotoxic Potential, Enzyme Activity and Microbial Community Function Diversity of the Rhizosphere Soils from Tea Plants with Continuous Cropping Years [J]. Chinese Journal of Tropical Crops, 2018, 39(5): 852-857. |
[14] | WANG Haibin, CHEN Xiaoting DING Li , QIU Fengyan, YE Jianghua, JIA Xiaoli, KONG Xianghai, HE Haibin. Effect of Soil Acidity on Microbial Diversity in Rhizospheric Soils of Tea Plants [J]. Chinese Journal of Tropical Crops, 2018, 39(3): 448-454. |
[15] | LIAO Daolong , HU Yanping YUN Tianhai XIE Dasen , ZHU Baibi , ZHANG Wen. Effects of Grafting and Organic Fertilizer on the Growth of Wax Gourd and Rhizosphere Soil Microbes [J]. Chinese Journal of Tropical Crops, 2018, 39(11): 2153-2159. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||