Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (3): 556-563.DOI: 10.3969/j.issn.1000-2561.2020.03.019

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

High-throughput Sequencing Analysis of Fungal Community in Rhizosphere Soils of Two Plants for Mushroon Cultivation (JUNCAO)

YE Wenyu1,2,XIE Xuze2,XU Yuying2,NI Mingyue3,HU Hongli3,YU Wenying2,Norvienyeku Justice2,LU Guodong1,3,*()   

  1. 1. China National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    3. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2019-05-14 Revised:2019-08-01 Online:2020-03-25 Published:2020-04-16
  • Contact: LU Guodong

Abstract:

High-throughput sequencing and bioinformatics analysis were used to analyze the composition of fungal communities in the rhizosphere and non-rhizosphere soils from the plants for mushroom cultivation. A total of 525 292 fungal ITS sequences from the samples were obtained. 7267 OTU (operational taxonomic units) could be grouped based on 97% sequence similarity as the threshold. The ACE of the fungal community was the lowest in the non-rhizosphere soil, and the rhizosphere soil of Lvzhou No. 1 was slightly higher than that in the rhizosphere soil of the plants for mushroom cultivation. The Shannon-wiener and Simpson values obtained for rhizosphere soils were higher than the values recorded from non-rhizosphere soils. Also, the Shannon-wiener and Simpson values for Lvzhou No. 1 rhizosphere soil was slightly higher compared to the values obtained for the rhizosphere soil of the plants for mushroom cultivation. This study further revealed Ascomycota, Basidiomycota, Zygomycota, Chytridiomycota, and Glomeromycota as the pre-dominant phylum present in all the rhizosphere soil samples. From class level analysis, we identified eight pre-dominant classes in all soil samples, including Eurotiomycetes, Sordariomycetes, Agaricomycetes, Leotiomycetes, Orbiliomycetes, Tremellomycetes, Dothideomycetes, and Wallemiomycetes. Nine pre-dominant genuses, including Talaromyces, Umbelopsis, Cladosporium, Cordana, Fusarium, Cryptococcus, Purpureocillium, Gibberella, and Ganoderma in all the soil samples were revealed. The results would provide insights into the prevailing upsurge in diversity in the rhizosphere fungi community compared to the non-rhizosphere soil and would serve as a reference for the characterization of rhizosphere fungi.

Key words: plants for mushroom cultivation (JUNCAO), rhizosphere soil, diversity of fungal communities, high- throughput sequencing

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