Assessment of Fungicides for <i>in vitro </i>and<i> in vivo</i> Activity Against <i>Xanthomanas campestris</i> pv. <i>mangiferaeindicae</i>
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (3): 538-543.DOI: 10.3969/j.issn.1000-2561.2020.03.016

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Assessment of Fungicides for in vitro and in vivo Activity Against Xanthomanas campestris pv. mangiferaeindicae

ZHAN Ligang1,YE Huochun2,ZHANG Jing2,3,YAN Chao2,LI Xiaoyu1,FENG Gang2,3,*()   

  1. 1. School of Life and Pharmaceutical Science, Hainan University, Haikou, Hainan 570228, China
    2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    3. Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 570100, China.
  • Received:2019-04-24 Revised:2019-07-03 Online:2020-03-25 Published:2020-04-16
  • Contact: FENG Gang


Mango bacterial black spot (MBBS) caused by Xanthomonas campestris pv. manganiferaeindicae (Xcm) is a risk plant disease in mango orchards. In order to screen an effective fungicide for controlling MBBS, the bacteriostatic activity of 22 fungicides against MBBS were assessed by th turbidimeter test in the laboratory, and the toxicity of the fungicides with good antibacterial activity was tested by the method of plate culture count and the control efficacy were carried out in the field. Laboratory bioassay results showed that the inhibitory effect of tetramycin, octylamine acetate, bromothalonil and benziothiazolinone was strong, with an inhibitory rate 90% treated with 2 mg/L, followed by pothioconazole and zhongsengmycin with an inhibitory rates up to 75% at 10 mg/L. Except for oxine-copper, the bacteriostatic activity of copper fungicides was relatively low. The toxicity of bromothalonil, benziothiazolinone, pothioconazole, kasugamycin and saisentong to Xcm was calculated, with EC50 of 0.048, 0.103, 0.900, 21.970 and 189.347 mg/L, respectively. At 30 days after treatment, the result of the field trial indicated that 20% zinc-thiazole SC 750 times solution (TS) had the highest control efficacy on the disease and its control effect reached 75%, followed by 3% benziothiazolinone ME, 20% pothioconazole SC and 25% bromothalonil EC reached above 70% control efficacy at 1000 TS. Beyond that, 46% copper hydroxide WG showed a better protective effect than other fungicides with lowest diseased leaf rate after treatment. Accordingly, copper hydroxide, zinc-thiazole, benziothiazolinone, pothioconazole and bromothalonil could be used as the chemicals for controlling mango bacterial black spot.

Key words: mango bacterial black spot, fungicides, antibacterial activity, control effect in vitro

CLC Number: