Analysis on Acid-aluminum Stress Response of Two Wild Alfalfa Resources in Yunnan

doi:10.3969/j.issn.1000-2561.2020.03.009

Abstract

Abstract:

Two wild alfalfa resources (Medicago lupulina, Medicago sativa) in Yunnan were used to study the effects of acid-aluminum stress on alfalfa seedling growth under water culture. The tolerance of the two alfalfa to acid and aluminum was compared by analyzing the changes of peroxidase and superoxide dismutase activities, root length, plant height, biomass, root vigor, chlorophyll content, malondialdehyde content under single and compound treatment of acid and aluminum. The seedling growth of the two alfalfas was inhibited significantly under acidity treatment. Comparation with pH 7.0 and pH 3.0, the root length, plant height, biomass and root activity of M. lupulina increased less than that of M. sativa. There were significant differences in the indexes for root length, plant height, biomass, root vigor between the two alfalfa resources under aluminum treatment. Seedling growth was seriously reduced with the increase of aluminum concentration. The influences of acidity and aluminum compound treatment on the seedling growth of the two alfalfa were greater than those of either stress in isolation. The growth of the two alfalfa was most severely inhibited under the pH 4.5, Al ³⁺ 300 mg/L treatment. Under the conditions of acid, aluminum or acid-aluminum stress treatments, the tolerance of M. lupulina to acid, aluminum and acid-aluminum was better than that of M. sativa from the point of view of the indexes of growth or decline of seedling growth, physiological indicators, inhibition rate.

Key words: alfalfa, acid-aluminum stress, seedling growth, antioxidant enzyme system

CLC Number:

S551+.7

WU Lifang,WEI Xiaomei,ZHANG Lifang,GUI Baolin. Analysis on Acid-aluminum Stress Response of Two Wild Alfalfa Resources in Yunnan[J]. Chinese Journal of Tropical Crops, 2020, 41(3): 482-488.

Figures/Tables 6

References 23

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pH	天蓝苜蓿（M. lupulin）				紫花苜蓿（M. sativa）
pH	根长 Root length/cm	株高 Plant height/cm	生物量 Biomass/mg	根系活力 Root vigor /(μg·g^-1·h^-1)	根长 Root length/cm	株高 Plant height/cm	生物量 Biomass/mg	根系活力 Root vigor /(μg·g^-1·h^-1)
3.0	0.56±0.04^e	0.64±0.04^e	21.2±0.26^e	46.49±1.24^e	0.53±0.03^e	0.50±0.08^e	20.3±0.57^e	31.20±0.94^e
4.0	0.92±0.01^d	0.82±0.09^d	24.2±0.26^d	59.00±1.56^d	0.87±0.03^d	0.73±0.06^d	23.4±0.67^d	46.46±1.26^d
5.0	1.38±0.04^c	1.31±0.11^c	25.8±0.46^c	85.72±1.49^c	1.18±0.10^c	1.35±0.06^c	24.8±0.70^c	65.04±1.97^c
6.0	1.83±0.06^b	1.89±0.04^b	27.1±0.26^b	109.26±1.34^b	1.65±0.35^b	1.71±0.07^b	26.6±0.62^b	74.16±1.05^b
7.0	2.03±0.09^a	2.08±0.04^a	28.7±0.35^a	123.89±1.39^a	2.02±0.05^a	2.08±0.04^a	28.0±0.65^a	89.63±0.81^a

pH	天蓝苜蓿（M. lupulin）				紫花苜蓿（M. sativa）
pH	根长 Root length/cm	株高 Plant height/cm	生物量 Biomass/mg	根系活力 Root vigor /(μg·g^-1·h^-1)	根长 Root length/cm	株高 Plant height/cm	生物量 Biomass/mg	根系活力 Root vigor /(μg·g^-1·h^-1)
3.0	0.56±0.04^e	0.64±0.04^e	21.2±0.26^e	46.49±1.24^e	0.53±0.03^e	0.50±0.08^e	20.3±0.57^e	31.20±0.94^e
4.0	0.92±0.01^d	0.82±0.09^d	24.2±0.26^d	59.00±1.56^d	0.87±0.03^d	0.73±0.06^d	23.4±0.67^d	46.46±1.26^d
5.0	1.38±0.04^c	1.31±0.11^c	25.8±0.46^c	85.72±1.49^c	1.18±0.10^c	1.35±0.06^c	24.8±0.70^c	65.04±1.97^c
6.0	1.83±0.06^b	1.89±0.04^b	27.1±0.26^b	109.26±1.34^b	1.65±0.35^b	1.71±0.07^b	26.6±0.62^b	74.16±1.05^b
7.0	2.03±0.09^a	2.08±0.04^a	28.7±0.35^a	123.89±1.39^a	2.02±0.05^a	2.08±0.04^a	28.0±0.65^a	89.63±0.81^a

pH	天蓝苜蓿（M. lupulina）				紫花苜蓿（M. sativa）
pH	叶绿素含量 Chlorophyll content/(mg·g^-1)	丙二醛含量 MDA content /(nmol·g^-1)	POD活性 POD activity /(U·g^-1·min^-1)	SOD活性 SOD activity /(U·g^-1)	叶绿素含量 Chlorophyll content/(mg·g^-1)	丙二醛含量MDA content /(nmol·g^-1)	POD活性 POD activity /(U·g^-1·min^-1)	SOD活性 SOD activity /(U·g^-1)
3.0	0.91±0.02^e	56.64±0.58^a	535.00±1.93^e	363.50±1.79^a	0.86±0.07^e	62.35±1.06^b	434.10±1.80^e	369.03±5.15^a
4.0	1.28±0.04^d	51.62±1.47^b	576.17±7.72^d	345.20±3.26^b	1.18±0.02^d	64.95±0.50^a	497.47±3.75^d	343.60±8.74^b
5.0	1.47±0.03^c	47.34±0.95^c	627.03±1.87^c	309.08±1.56^c	1.33±0.07^c	55.97±1.54^c	573.53±2.44^c	318.93±3.54^c
6.0	1.76±0.04^b	42.64±0.58^d	667.33±9.45^b	264.65±3.33^d	1.71±0.03^b	46.51±1.24^d	686.53±5.08^a	280.83±6.93^d
7.0	1.94±006^a	37.67±2.05^e	731.00±3.08^a	231.57±1.91^e	2.08±0.10^a	37.69±1.38^e	653.93±1.76^b	246.20±8.61^e

pH	天蓝苜蓿（M. lupulina）				紫花苜蓿（M. sativa）
pH	叶绿素含量 Chlorophyll content/(mg·g^-1)	丙二醛含量 MDA content /(nmol·g^-1)	POD活性 POD activity /(U·g^-1·min^-1)	SOD活性 SOD activity /(U·g^-1)	叶绿素含量 Chlorophyll content/(mg·g^-1)	丙二醛含量MDA content /(nmol·g^-1)	POD活性 POD activity /(U·g^-1·min^-1)	SOD活性 SOD activity /(U·g^-1)
3.0	0.91±0.02^e	56.64±0.58^a	535.00±1.93^e	363.50±1.79^a	0.86±0.07^e	62.35±1.06^b	434.10±1.80^e	369.03±5.15^a
4.0	1.28±0.04^d	51.62±1.47^b	576.17±7.72^d	345.20±3.26^b	1.18±0.02^d	64.95±0.50^a	497.47±3.75^d	343.60±8.74^b
5.0	1.47±0.03^c	47.34±0.95^c	627.03±1.87^c	309.08±1.56^c	1.33±0.07^c	55.97±1.54^c	573.53±2.44^c	318.93±3.54^c
6.0	1.76±0.04^b	42.64±0.58^d	667.33±9.45^b	264.65±3.33^d	1.71±0.03^b	46.51±1.24^d	686.53±5.08^a	280.83±6.93^d
7.0	1.94±006^a	37.67±2.05^e	731.00±3.08^a	231.57±1.91^e	2.08±0.10^a	37.69±1.38^e	653.93±1.76^b	246.20±8.61^e

Al³⁺ /(mg·L^-1)	天蓝苜蓿（M. lupulina）				紫花苜蓿（M. sativa）
Al³⁺ /(mg·L^-1)	根长 Root length/cm	株高 Plant height/cm	生物量 Biomass/mg	根系活力 Root vigor/ (μg·g^-1·h^-1)	根长 Root length/cm	株高 Plant height/cm	生物量 Biomass/mg	根系活力 Root vigor/ (μg·g^-1·h^-1)
0	2.00±0.09^a	2.12±0.09^a	28.37±1.05^a	111.70±1.59^a	2.05±0.09^a	2.26±0.05^a	28.77±0.99^a	92.72±4.19^a
50	1.71±0.02^b	1.93±0.02^b	26.30±0.40^b	86.69±2.96^b	1.65±0.02^b	1.92±0.04^b	24.87±0.55^b	71.83±1.55^b
100	1.43±0.02^c	1.68±0.05^c	20.77±0.57^c	76.18±0.97^c	1.38±0.02^c	1.64±0.09^c	19.13±0.25^c	65.75±1.63^c
200	1.21±0.02^d	1.47±0.08^d	16.87±0.45^d	64.47±2.51^d	1.11±0.02^d	1.41±0.02^d	14.90±0.85^d	56.46±0.88^d
300	0.97±0.05^e	1.16±0.06^e	12.00±0.36^e	49.54±0.80^e	0.90±0.03^e	1.06±0.08^e	10.20±0.56^e	44.96±1.96^e
400	0.72±0.03^f	0.85±0.05^f	9.10±0.36^f	42.19±2.00^f	0.66±0.04^f	0.78±0.03^f	8.67±0.45^f	35.13±2.63^f
500	0.55±0.04^g	0.68±0.04^g	8.17±0.21^f	36.30±2.37^g	0.47±0.06^g	0.65±0.05^g	8.07±0.35^f	25.95±1.77^g