Ploidy Identification of Germplasm Resources in <i>Hevea brasiliensis </i>(Willd. ex A. Juss.) Müll. Arg.
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (3): 457-463.DOI: 10.3969/j.issn.1000-2561.2020.03.006

• Crop Culture and Nutrition, Genetic Breeding • Previous Articles     Next Articles

Ploidy Identification of Germplasm Resources in Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg.

ZHANG Yuanyuan1,2,3,4,5,PU Shouqin6,7,*,HU Yanshi1,2,3,4,5,FANG Jialin1,2,3,4,5,HUANG Xiao1,2,3,4,5,LI Weiguo1,2,3,4,5,**(),HUANG Huasun1,2,3,4,5   

  1. 1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    2. State Centre for Rubber Breeding, Haikou, Hainan 571101, China
    3. Rubber Tree Germplasm Repository, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
    4. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
    5. State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Haikou, Hainan 571101, China;
    6. College of Forestry, Hainan University, Haikou, Hainan 570228, China
    7. Engineering Research Center of Rare and Precious Tree Species in Hainan Province, Haikou, Hainan 570228, China
  • Received:2019-04-28 Revised:2019-07-04 Online:2020-03-25 Published:2020-04-16
  • Contact: PU Shouqin,LI Weiguo

Abstract:

Ploidy of rubber tree germplasm in the Rubber Tree Germplasm Repository and the ratio of tetraploid cells in chimeras were analyzed by flow cytometry, and the stomatal traits of different ploidy were analyzed. Eleven triploids and one tetraploid were found from 5177 1981’IRRDB germplasms, nine triploids and two tetraploid and fourteen chimeras were found from 552 Wickham germplasms. The ratio of tetraploid cells in fourteen chimeras ranged from 49.06% to 78.26%. The difference of stomatal length, stomatal width, and stomatal density between different ploidy was significant (P<0.01), the rank of stomatal length and stomatal width was tetraploid>triploid>chimeras, while the stomatal density was reversed. This study was valuable for the use of tetraploid germplasm resources for cross breeding between the ploidy of rubber trees.

Key words: Hevea brasiliensis, germplasm resources, polyploid, flow cytometry, stomatal traits

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