Ultrasonic-Microwave Synergistic Extraction of Pectin from the Peel of Passiflora edulis

doi:10.3969/j.issn.1000-2561.2020.02.025

Abstract

Abstract:

The extracting rate of the pectin was used as the analytical index, and the ultrasonic-microwave synergistic extraction of dried fruit peel pectin from passion fruit was optimized by the response surface methodology. The optimum process parameters of the ultrasonic-microwave synergistic extraction of the peel of passion fruit were obtained, namely liquid to material ratio 30 mL/g, pH 2.0, temperature 50 ℃, water bath 60 min, ultrasonic power 50 W, microwave power 600 W, ultrasonic-microwave time 8.0 min. Under the condition, the yield of pectin could reach (12.14±0.06)%. Compared with water extraction, ultrasonic and microwave methods, the extraction rate of ultrasonic-microwave synergy method increased by 47.33%, 34.74% and 23.50%, respectively. The esterification degree of the four extraction methods was≥50%, indicating that the peel pectin of passion fruit was a high methoxyl onein. The results of scanning electron microscopy showed that the cell wall of the fruit peel was broken more thoroughly under the synergistic effect of ultrasound-microwave, which was beneficial to the dissolution of pectin.

Key words: peel of Passiflora edulis, pectin, ultrasonic-microwave synergistic extraction, scanning electron microscopy

CLC Number:

S667.9

LI Ying,LIU Xialei,LIN Yaxin,CHEN Xuemei. Ultrasonic-Microwave Synergistic Extraction of Pectin from the Peel of Passiflora edulis[J]. Chinese Journal of Tropical Crops, 2020, 41(2): 386-393.

Figures/Tables 8

References 26

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水平 Level	因素Factors
水平 Level	(A)液料比Liquid-to-solid ratio/(mL·g^-1)	(B) pH	(C)微波功率 Microwave power/W	(D)微波时间 Microwave time/min
-1	20	1.5	500	6
0	30	2.0	600	8
1	40	2.5	700	10

水平 Level	因素Factors
水平 Level	(A)液料比Liquid-to-solid ratio/(mL·g^-1)	(B) pH	(C)微波功率 Microwave power/W	(D)微波时间 Microwave time/min
-1	20	1.5	500	6
0	30	2.0	600	8
1	40	2.5	700	10

方差来源 Variance source	平方和 Sum of squares	自由度 DF	均方 Mean square	F值 F value	Prob>F	显著性 Significant
模型	9.87	14	0.71	106.53	<0.0001	**
A	0.015	1	0.015	2.22	0.1584
B	0.075	1	0.075	11.36	0.0046	**
C	0.051	1	0.051	7.66	0.0151	*
D	0.049	1	0.049	7.46	0.0162	*
AB	0.10	1	0.10	15.47	0.0015	**
AC	0.040	1	0.040	6.04	0.0276	*
AD	0.12	1	0.12	18.50	0.0007	**
BC	0.036	1	0.036	5.45	0.0349	*
BD	2.15	1	2.15	324.16	<0.0001	**
CD	0.014	1	0.014	2.17	0.1624
A²	3.44	1	3.442	519.11	<0.0001	**
B²	2.29	1	2.29	345.87	<0.0001	**
C²	1.17	1	1.17	177.31	<0.0001	**
D²	3.96	1	3.96	598.60	<0.0001	**
残差	0.093	14	6.621E-003
失拟项	0.065	10	6.529E-003	0.95	0.5703	不显著
纯误差	0.027	4	6.850E-003
总和	9.97	28

方差来源 Variance source	平方和 Sum of squares	自由度 DF	均方 Mean square	F值 F value	Prob>F	显著性 Significant
模型	9.87	14	0.71	106.53	<0.0001	**
A	0.015	1	0.015	2.22	0.1584
B	0.075	1	0.075	11.36	0.0046	**
C	0.051	1	0.051	7.66	0.0151	*
D	0.049	1	0.049	7.46	0.0162	*
AB	0.10	1	0.10	15.47	0.0015	**
AC	0.040	1	0.040	6.04	0.0276	*
AD	0.12	1	0.12	18.50	0.0007	**
BC	0.036	1	0.036	5.45	0.0349	*
BD	2.15	1	2.15	324.16	<0.0001	**
CD	0.014	1	0.014	2.17	0.1624
A²	3.44	1	3.442	519.11	<0.0001	**
B²	2.29	1	2.29	345.87	<0.0001	**
C²	1.17	1	1.17	177.31	<0.0001	**
D²	3.96	1	3.96	598.60	<0.0001	**
残差	0.093	14	6.621E-003
失拟项	0.065	10	6.529E-003	0.95	0.5703	不显著
纯误差	0.027	4	6.850E-003
总和	9.97	28