Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (2): 339-345.DOI: 10.3969/j.issn.1000-2561.2020.02.018

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Optimization of Fermentation Conditions of Antagonistic Actinomycetes FS-4

DUAN Yajie1,MEI Zhigang2,SUN Dequan1,LI Weiming1,PANG Zhencai1,HU Huigang1,*()   

  1. 1. South Asia Tropical Crops Research Institute, Chinese Academy of Tropical Agriculture Sciences / Key Laboratory of Tropical Fruit’s Biology, Ministry of Agriculture and Rural Affairs, Zhanjiang, Guangdong 524091, China
    2. School of Mechanical and Power Engineering, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China
  • Received:2019-04-02 Revised:2019-07-31 Online:2020-02-25 Published:2020-03-21
  • Contact: HU Huigang


The screening of antagonistic bacteria from soil microorganisms in the areas with banana Fusarium wilt is of great practical significance for controlling this disease. In this report, a strain, designated FS-4, was isolated from healthy banana rhizosphere soil in the area affected by Fusarium wilt. Based on Gause’s No. 1 synthetic medium, the optimization of medium, single factor experiments and response surface experiments were conducted to maximize the production of the antibacterial substances of actinomycete FS-4. The best fermentation medium and fermentation conditions were as follows: sucrose 2.4%, peptone 0.5%, K2HPO4 0.05%, NaCl 0.05%, MgSO4 0.05%, fermentation temperature 28 ℃, initial pH 7. Under the conditions, the inhibition zone diameter of actinomycete FS-4 fermentation filtrate reached 27.1 mm on the test plates of Bacillus subtilis after 62 h incubation

Key words: actinomycetes FS-4, fermentation conditions, response surface method

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