Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (2): 323-332.DOI: 10.3969/j.issn.1000-2561.2020.02.016

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Analysis of Differentially Expressed Genes in Lettuce Leaves Under Different Optical Spectrophotometric Films Based on Transcriptome Sequencing

ZHOU Niannian1,GAO Jie1,DING Mingzhu1,AN Yulan1,ZHAI Keqing1,SHI Jiayin2,GAN Defang1,**(),LIU Wen3   

  1. 1. School of Horticulture, Anhui Agricultural University, Hefei, Anhui 230036, China
    2. Agricultural Station of Jingong Town, Huaining, Anqing, Anhui 246100, China
    3. School of Physical Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China
  • Received:2019-06-13 Revised:2019-07-03 Online:2020-02-25 Published:2020-03-21
  • Contact: GAN Defang


New photovoltaic agriculture can generate photovoltaic power under the light demand of crop growth. To study the differential gene expression in lettuce leaves under different spectrophotometric films, the ‘Adina’ lettuce variety was used as the experimental material, and the gene expression level of lettuce leaves under the conditions of red and blue filter film (RBFF) and far infrared cut-off film (RICF) was analyzed by the transcriptome sequencing (RNA-seq) technique. The results showed that 2685 genes were differentially expressed in RBFF, including 1380 up-regulated genes and 1305 down-regulated genes; 3264 genes were differentially expressed in RICF, of which 881 were up-regulation and 2383 down-regulation. Further analysis revealed that 1498 differentially expressed genes were expressed in both RBFF and RICF, 1187 genes were differentially expressed in the RBFF, while 1766 were differentially expressed in RICF. Ten genes related to light stress were screened from the differentially expressed genes, and the reliability of transcriptome data was verified by qRT-PCR. The results of expression analysis of light response related genes showed that most of the genes under red-blue filter film were expressed in large quantities, and the expression reached peak at 15 d; under far infrared cut-off film, the expression of three genes was higher, while the expression of others was lower. The expression of most genes reached peak at 15-21 d. This study would provide a theoretical basis and data support for further exploring photoresponsive genes in the lettuce genome and revealing the molecular mechanism of the lettuce response to light.

Key words: lettuce, optical spectrophotometric film, transcriptome analysis, quantitative real-time PCR (qRT-PCR)

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