Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (2): 292-299.DOI: 10.3969/j.issn.1000-2561.2020.02.012

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Cloning and Characterization of Mugsp7, a β-1,3-glucanase Gene of Wild Banana Germplasm from Sanming, Fujian, China, Under Cold Treatment

XU Xiaoping,XIE Yanping,CHEN Fanglan,CHEN Xiaohui,CHEN Yukun,ZHANG Zihao,CHEN Chunzhen,LIN Yuling,LAI Zhongxiong()   

  1. Institute of Horticultural Biotechnology/Institute of Subtropical Fruits, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Received:2019-04-02 Revised:2019-08-28 Online:2020-02-25 Published:2020-03-21
  • Contact: LAI Zhongxiong


The tissue culture seedlings of a wild banana germplasm from Sanming were used to clone the cDNA and gDNA sequences of β-1,3-glucanase gene Mugsp7 by RT-PCR. Bioinformatics and qRT-PCR analysis of the expression at different low temperature were also carried out, and the enzyme activity of mperatureureent lowthe leaves was further determined by treating at 8 ℃ for 1, 2, 3, 4 and 5 days. The gDNA sequence of Mugsp7 was 1132 bp with an open reading frame (ORF) of 984 bp and one intron of 148 bp, which encoding 327 amino acids. Bioinformatics analysis showed that Mugsp7 was an acidic hydrophobic stable protein, and there was no signal peptide. The amino acid sequence of Mugsp7 shared significant similarity with that in Musa acuminata, Zostera marina, Maize, Hordeum vulgare L , Oryza sativa on the same branch, and it was closely related to Musa acuminata. The expression pattern of Mugsp7 under different low temperature treatments was different, suggesting that Mugsp7 was a low temperature stress related gene.

Key words: wild banana germplasm from Sanming, β-1,3-glucanase, Mugsp7, gene cloning, low temperature stress, qPCR

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