Genetic Diversity Analysis and DNA Fingerprinting Construction of Waterlily Germplasm Resources
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (2): 258-266.DOI: 10.3969/j.issn.1000-2561.2020.02.008

• Crop Culture and Nutrition, Genetic Breeding • Previous Articles     Next Articles

Genetic Diversity Analysis and DNA Fingerprinting Construction of Waterlily Germplasm Resources

SU Qun1,YANG Yahan1,2,TIAN Min3,BU Zhaoyang1,MAO Liyan4,ZHANG Jinzhong1,PAN Jiechun2,LU Jiashi1,**()   

  1. 1. Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
    2. Guangxi University, Nanning, Guangxi 530004, China
    3. Flower Research Institute, Yunnan Academy of Agricultural Sciences / National Engineering Research Center for Ornamental Horticulture, Kunming, Yunnan 650200, China
    4. Guangxi Subtropical Crops Research Institute, Nanning, Guangxi 530001, China
  • Received:2019-03-11 Revised:2019-06-04 Online:2020-02-25 Published:2020-03-21
  • Contact: LU Jiashi


The genetic diversity of 46 waterlily germplasm resources introduced within China and abroad was analyzed by the ISSR molecular marker technology, and the DNA fingerprints of the original species and varieties were constructed in order to clarify the genetic relationship and provide a theoretical basis for the classification and identification of waterlily, hybrid breeding and functional gene mining, utilization and map-based cloning. The results showed that 10 primers with high polymorphism and good repeatability were screened out from 100 ISSR primers. A total of 281 bands were amplified from 46 waterlily germplasm resources, with an average of 28.1 bands per primer, 281 polymorphic bands and 100% polymorphic ratio. The average Shannon information index (I) was 0.4197, the average Nei's gene diversity index (H) was 0.2657, and the average effective allele number (N) was 1.4139, which showed abundant genetic diversity. The genetic similarity coefficient was between 0.51 and 0.98. Based on the genetic similarity coefficient, a clustering tree was constructed to reveal the genetic relationship of the 46 waterlily germplasm resources. When the similarity coefficient was 0.68, all the tested materials could be clustered into six groups. DNA fingerprints were constructed for 24 original species and varieties of waterlily, and different waterlily germplasms could be identified according to the differences of DNA fingerprints.

Key words: waterlily germplasm resources, ISSR molecular markers, genetic analysis, DNA fingerprintings

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