Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (2): 244-251.DOI: 10.3969/j.issn.1000-2561.2020.02.006

• Crop Culture and Nutrition, Genetic Breeding • Previous Articles     Next Articles

Optimization of Regeneration System of Main Sweet Patato Cultivars in Hainan, China

SUN Yanbo1,ZHU Zhixin2,HUANG Ting1,LI Siming1,ZHU Guopeng1,*()   

  1. 1. School of Horticulture, Hainan University, Haikou, Hainan 570228, China
    2. School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan 570228, China
  • Received:2019-05-13 Revised:2019-07-02 Online:2020-02-25 Published:2020-03-21
  • Contact: ZHU Guopeng


In this study, the main sweet potato varieties planted in tropical areas, including ‘Xinxiang’, ‘Guangshu 87’, ‘Chuanshan Zi’, ‘Ning Zishu 1’, ‘Shulv 1’, ‘Gaoxi 14’ and ‘Sanjiao Ning’ were used as the materials. The selection of explants, disinfection schemes, medium formulation and anti-browning agent were explored. The optimum regeneration system of virus-free and rapid propagation was established for the main sweet potato varieties. The proliferation rate and survival rate of lateral buds were the highest among different parts of explants. In treatments of different explant disinfection, 75% alcohol for 60 s, 2% sodium hypochlorite for 15 min, and 0.1% mercury chloride for 15 min exhibited the lowest contamination rate. The medium formula with the shortest rooting time was as follows: MS+0.05 mg/L NAA+0.1 mg/L GA3. The medium formula with the fastest growth of stem length was as follows: MS+0.1 mg/L NAA+1 mg/L 6-BA+0.5 mg/L GA3. The medium formula with the fastest growth rate of dry weight was as follows: MS+0.1 mg/L NAA+1.5 mg/L 6-BA+0.5 mg/L GA3. Adding 5-6 g/L sodium thiosulfate and 1.25 g/L polyvinylpyrrolidone to the medium could effectively inhibit the browning of explants.

Key words: sweet potato, regeneration system, medium formula, browning of explants

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