Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2020, Vol. 41 ›› Issue (1): 110-115.DOI: 10.3969/j.issn.1000-2561.2020.01.016

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Vitrification of Regenerated Shoots of Tagetes erecta L. in Pigment Marigold

WU Lifang1,TIAN Xuelian1,WEI Xiaomei1,LU Xiaochun1,QIAN Shaofang2,GUI Baolin3   

  1. 1. College of Biological Resource and Food Engineering, Qujing Normal University / Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing, Yunnan 655011, China
    2. Qujing BoHao Biotech Co., Ltd., Zhanyi, Yunnan 655331, China
    3. Soil Fertilizer Workstation, Xundian Agricultural Bureau, Xundian, Yunnan 650224, China
  • Received:2019-01-28 Revised:2019-07-10 Online:2020-01-25 Published:2020-01-21

Abstract:

The stem with axillary buds from Tagetes erecta L. was used as the explants, and the effects of sucrose, AgNO3, agar, plant gel, activated carbon and different sealing materials on shoot proliferation and vitrification were studied in this paper. The results showed that 30 g/L sucrose was suitable for the growth of the plantlet. The vitrification rate decreased with sucrose concentration increased (10-60 g/L). The vitrification rate was only 9.13% with sucrose concentration at 60 g/L. AgNO3 had the effect of inhibiting the growth of glass seedlings. 5.0 g/L AgNO3 had a vitrification rate as low as 9.81%. The quantity of activated carbon (AC) was most obvious to vitrification, 5.0 g/L AC was most serious for vitrification, and the vitrification rate was as high as 38.18%. Agar and plant gels showed a same trend for bud proliferation and vitrification. Low concentration was conducive for shoot proliferation and high concentration could effectively prevent the occurrence of vitrification. Vitrification rate was the lowest, only 8.31% for 10 g/L agar. The shoot proliferation coefficient was as high as 3.35 for 2.0 g/L plant gel, and the vitrification rate was as low as 6.67% for 5.0 g/L plant gel. When cotton plug plus double kraft paper plus cotton thread was used as the sealing materials, the shoot proliferation coefficient was as high as 3.24 and the vitrification rate was as low as 8.46%.

Key words: pigment marigold, regenerated shoots, vitrification, proliferation

CLC Number: