Effects of Water-retaining Agent and Nitrogen Fertilizer on Photosynthetic Characteristics and Chlorophyll Fluorescence Characteristics of Bambusa tuldoides cv. Swolleninternode

doi:10.3969/j.issn.1000-2561.2020.01.012

Abstract

Abstract:

In this study, the effects of nitrogen fertilizer (150, 300 and 450 g/cluster) and water-retaining agent (180 g/cluster) combined with nitrogen fertilizer (150, 300 and 450 g/cluster) on chlorophyll, photosynthetic parameters and chlorophyll fluorescence parameters of three-year-old Bambusa tuldoides cv. Swolleninternode were studied, and the comprehensive evaluation of different treatments was carried out by principal component analysis. The results showed that chlorophyll content increased with the increase of nitrogen fertilizer, and the content of water-retaining agent mixed with nitrogen fertilizer was lower than that of nitrogen fertilizer alone. Net photosynthetic rate (P_n), transpiration rate (T_r), stomatal conductance (G_s), intercellular carbon dioxide concentration (C_i), water use efficiency (WUE) of the leaf increased with the increase of nitrogen fertilizer, and the value of photosynthetic parameters was higher under the application of water retaining agent than that under the application of nitrogen fertilizer alone. The initial fluorescence (F_o) of all fertilization treatments was higher than that of CK. With the increase of nitrogen fertilizer, the maximum fluorescence (F_m), variable fluorescence (F_v), potential activity (F_v/F_o), maximum photochemical efficiency (F_v/F_m), actual photochemical yield (PS II), photosynthetic electron transfer rate (ETR), photochemical quenching coefficient (qP) and non-photochemical quenching coefficient (qN) also increased gradually, while under the same amount of nitrogen fertilizer, the values increased with the increase of water retaining agent. The principal component analysis scores of CK, A, B, C, D, E and F was -12.57, -8.15, -3.89, 3.15, 0.12, 10.02 and 11.32, respectively. Therefore, the application of nitrogen fertilizer promoted the growth of B. tuldoides cv. Swolleninternode, and the effect of adding water-retaining agent was better than that of applying nitrogen fertilizer alone. The comprehensive score F was the highest, meaning the combination of water-retaining agent (180 g/cluster) and nitrogen fertilizer (450 g/cluster) was the best.

Key words: Bambusa tuldoides cv. Swolleninternode, water retaining agent, nitrogen fertilizer, photosynthetic characteristics, chlorophyll fluorescence characteristics

CLC Number:

S795

ZHANG Li,ZHOU Chengcheng,XU Wenda,JIANG Denghui,RONG Jundong,YE Longtai,HE Tianyou,ZHENG Yushan. Effects of Water-retaining Agent and Nitrogen Fertilizer on Photosynthetic Characteristics and Chlorophyll Fluorescence Characteristics of Bambusa tuldoides cv. Swolleninternode[J]. Chinese Journal of Tropical Crops, 2020, 41(1): 83-88.

Figures/Tables 7

References 21

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变量 Variable	平方和 Sum of squares	自由度 df	均方 Mean square	F值 F-value	显著性水平 Significance
Chl(a+b)	0.92	6	0.15	176.19	0
P_n	87.40	6	14.57	77.41	0
G_s	0.01	6	0.002	59.62	0
C_i	728 759.41	6	121 459.90	43.83	0
T_r	8.01	6	1.33	10.70	0
WUE	2.46	6	0.41	19.98	0
F_o	3 665.33	6	610.89	7.56	0.001
F_v/F_m	0.22	6	0.04	79.68	0
F_v/F_o	4.24	6	0.71	31.29	0
Φ_PSⅡ	0.08	6	0.01	48.21	0
ETR	273.02	6	45.50	63.87	0
qP	0.02	6	0.00	39.78	0
qN	0.05	6	0.01	39.27	0

变量 Variable	平方和 Sum of squares	自由度 df	均方 Mean square	F值 F-value	显著性水平 Significance
Chl(a+b)	0.92	6	0.15	176.19	0
P_n	87.40	6	14.57	77.41	0
G_s	0.01	6	0.002	59.62	0
C_i	728 759.41	6	121 459.90	43.83	0
T_r	8.01	6	1.33	10.70	0
WUE	2.46	6	0.41	19.98	0
F_o	3 665.33	6	610.89	7.56	0.001
F_v/F_m	0.22	6	0.04	79.68	0
F_v/F_o	4.24	6	0.71	31.29	0
Φ_PSⅡ	0.08	6	0.01	48.21	0
ETR	273.02	6	45.50	63.87	0
qP	0.02	6	0.00	39.78	0
qN	0.05	6	0.01	39.27	0

处理Treatments	Chl(a+b)/(mg·g^-1)
CK	2.02±0.02^f
A	2.23±0.02^e
B	2.46±0.03^c
C	2.68±0.02^a
D	2.22±0.04^e
E	2.41±0.03^d
F	2.54±0.02^b

处理Treatments	Chl(a+b)/(mg·g^-1)
CK	2.02±0.02^f
A	2.23±0.02^e
B	2.46±0.03^c
C	2.68±0.02^a
D	2.22±0.04^e
E	2.41±0.03^d
F	2.54±0.02^b

处理 Treatments	P_n /(μmol·m^-2∙s^-1)	G_s /(mol∙m^-2∙s^-1)	C_i /(μmol·m^-2∙s^-1)	T_r /(mmol∙m^-2∙s^-1)	WUE /(μmol∙m^-2∙s^-1)
CK	2.69±0.07^e	0.035±0.000 09^d	298.30±0.46^c	2.38±0.11^b	1.12±0.11^d
A	3.59±0.07^d	0.039±0.000 03^d	334.89±0.42^dc	2.44±0.17^b	1.48±0.10^c
B	4.18±0.08^d	0.037±0.000 04^d	405.49±0.42^d	2.90±0.13^b	1.44±0.10^c
C	6.72±0.07^b	0.075±0.000 05^b	563.66±0.39^c	3.67±0.14^a	1.84±0.09^b
D	5.67±0.07^c	0.052±0.000 06^c	568.06±0.48^c	3.03±0.12^b	1.86±0.12^b
E	7.95±0.07^a	0.090±0.000 07^a	841.58±0.44^b	3.69±0.12^a	2.15±0.15^a
F	8.41±0.06^a	0.100±0.000 08^a	841.58±0.43^a	4.13±0.16^a	2.04±0.18^b