Determination of Dimethomorph and Gibberellin A3 Residues in Fruits and Vegetables by Liquid Chromatography

doi:10.3969/j.issn.1000-2561.2019.12.027

Abstract

Abstract:

A simple, sensitive and reproducible high-performance liquid chromatographic method was developed for determining dimethomorph and gibberellin A3 residues in fruit and vegetable samples (strawberry, watermelon and tomatoes). The samples were extracted with ethyl acetate under acid conditions (pH 2.5), cleaned up by florisil solid-phase extraction cartridge, and analyzed by high-performance liquid chromatography with ultraviolet detection. There were good linear correlations between the peak area and the corresponding concentration of dimethomorph and gibberellin A3 in the range of 0.025-1.00 μg/mL and the correlation coefficient was above 0.9995. The average recovery of dimethomorph and gibberellin A3 in three levels (0.025, 0.050 and 0.100 mg/kg) was between 81.2 and 91.3. The limit of detection of the dimethomorph and gibberellin A3 was 0.01 mg/kg and 0.02 mg/kg, respectively.

Key words: dimethomorph, gibberellin A3, HPLC, vegetable, fruit

CLC Number:

Q949.748.5

GUO Hongbin,WANG Mingyue,ZHANG Chunyan,LIN Ling,YE Jianzhi,YANG Chunliang. Determination of Dimethomorph and Gibberellin A3 Residues in Fruits and Vegetables by Liquid Chromatography[J]. Chinese Journal of Tropical Crops, 2019, 40(12): 2512-2518.

Figures/Tables 8

References 16

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提取溶剂 Extracting solvents	回收率Recovery rates/%
提取溶剂 Extracting solvents	草莓Strawberry	西瓜Watermelon	西红柿Tomatoe
甲醇	32.3±5.6	23.9±2.7	31.2±2.6
乙腈	60.7±3.6	67.6±3.0	61.9±3.5
正己烷	30.3±5.1	32.6±3.2	31.2±4.6
乙酸乙酯	76.7±3.1	84.5±2.7	82.7±4.1
乙酸乙酯（pH 2.5）	84.3±4.8	86.5±1.6	87.3±5.5

提取溶剂 Extracting solvents	回收率Recovery rates/%
提取溶剂 Extracting solvents	草莓Strawberry	西瓜Watermelon	西红柿Tomatoe
甲醇	32.3±5.6	23.9±2.7	31.2±2.6
乙腈	60.7±3.6	67.6±3.0	61.9±3.5
正己烷	30.3±5.1	32.6±3.2	31.2±4.6
乙酸乙酯	76.7±3.1	84.5±2.7	82.7±4.1
乙酸乙酯（pH 2.5）	84.3±4.8	86.5±1.6	87.3±5.5

提取溶剂 Extracting solvents	回收率Recovery rates/%
提取溶剂 Extracting solvents	草莓Strawberry	西瓜Watermelon	西红柿Tomatoes
甲醇	31.7±5.1	31.7±2.3	33.6±2.2
乙腈	55.9±1.7	61.5±2.3	51.8±2.6
正己烷	22.5±6.2	33.7±2.6	32.6±2.7
乙酸乙酯	73.5±2.9	80.3±1.5	79.9±2.3
乙酸乙酯（pH 2.5）	82.4±6.7	87.6±2.0	91.3±2.8

提取溶剂 Extracting solvents	回收率Recovery rates/%
提取溶剂 Extracting solvents	草莓Strawberry	西瓜Watermelon	西红柿Tomatoes
甲醇	31.7±5.1	31.7±2.3	33.6±2.2
乙腈	55.9±1.7	61.5±2.3	51.8±2.6
正己烷	22.5±6.2	33.7±2.6	32.6±2.7
乙酸乙酯	73.5±2.9	80.3±1.5	79.9±2.3
乙酸乙酯（pH 2.5）	82.4±6.7	87.6±2.0	91.3±2.8

样品 Sample	添加浓度 Spiked level/ (mg·kg^-1)	回收率Recovery rates/%
样品 Sample	添加浓度 Spiked level/ (mg·kg^-1)	Dimethomorph	Gibberellin A3
草莓	0.025	84.3±4.8	82.4±6.7
	0.050	87.8±2.3	87.8±3.5
	0.100	83.2±3.1	83.0±1.2
	1.000	88.7±1.6	81.8±3.1
西瓜	0.025	86.5±1.6	87.6±2.0
	0.050	88.8±1.5	88.8±4.3
	0.100	82.6±2.1	81.2±2.2
	1.000	91.6±4.3	82.5±1.1
西红柿	0.025	87.3±5.5	91.3±2.8
	0.050	88.6±4.2	89.8±3.1
	0.100	82.2±0.2	85.3±0.8
	1.000	91.2±2.0	83.2±1.5