Determination of Dimethomorph and Gibberellin A3 Residues in Fruits and Vegetables by Liquid Chromatography
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (12): 2512-2518.DOI: 10.3969/j.issn.1000-2561.2019.12.027

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Determination of Dimethomorph and Gibberellin A3 Residues in Fruits and Vegetables by Liquid Chromatography

GUO Hongbin1,WANG Mingyue2,ZHANG Chunyan3,LIN Ling1,YE Jianzhi1,YANG Chunliang1,*()   

  1. 1. Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Zhanjiang, Guangdong 524001, China
    2. Analysis and Testing Center, CATAS, Haikou, Hainan 571101, China
    3. College of Tropical Crops, Yunnan Agricultural University, Pu°er, Yunnan 665000, China
  • Received:2019-01-06 Revised:2019-06-20 Online:2019-12-25 Published:2019-12-20
  • Contact: YANG Chunliang

Abstract:

A simple, sensitive and reproducible high-performance liquid chromatographic method was developed for determining dimethomorph and gibberellin A3 residues in fruit and vegetable samples (strawberry, watermelon and tomatoes). The samples were extracted with ethyl acetate under acid conditions (pH 2.5), cleaned up by florisil solid-phase extraction cartridge, and analyzed by high-performance liquid chromatography with ultraviolet detection. There were good linear correlations between the peak area and the corresponding concentration of dimethomorph and gibberellin A3 in the range of 0.025-1.00 μg/mL and the correlation coefficient was above 0.9995. The average recovery of dimethomorph and gibberellin A3 in three levels (0.025, 0.050 and 0.100 mg/kg) was between 81.2 and 91.3. The limit of detection of the dimethomorph and gibberellin A3 was 0.01 mg/kg and 0.02 mg/kg, respectively.

Key words: dimethomorph, gibberellin A3, HPLC, vegetable, fruit

CLC Number: