Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (12): 2447-2455.DOI: 10.3969/j.issn.1000-2561.2019.12.019

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Effects of smy1 Gene Knockout on Physiological Function of Fusarium oxysporum f. sp. cubense race 4

HE Zhuang1,2,QI Yanxiang2,ZENG Fanyun2,DING Zhaojian2,LIANG Junwei1,2,ZHANG Xin2,PENG Jun2,XIE Peilan2,XIE Yixian2,*()   

  1. 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2019-01-15 Revised:2019-05-11 Online:2019-12-25 Published:2019-12-20
  • Contact: XIE Yixian


Smy1 is a kinesin involved in the regulation of fungal growth. In the study, the smy1 kinesin gene of Fusarium oxysporum f. sp. cubense race 4 (Foc 4) was cloned. The smy1 knockout mutant of Foc 4 was obtained by homologous recombination technique. Besides observing the mycelial morphological characteristic, growth rate and sporulation of the mutants, pathogenicity to banana seedlings and sensitivity to fungicide were studied in order to reveal the role of smy1 played in the growth and development of F. oxysporum and resistance to fungicide. The results showed that, compared with the Foc 4 wild-type strain, the knockout mutants grew slowly, the mycelial morphology changed, the sporulation increased, and the pathogenicity to banana plantlets seedlings decreased, but the resistance level to fungicide changed little. This indicates that the smy1 gene may play an important role in the growth, sporulation and pathogenicity in Foc 4.

Key words: Fusarium oxysporum, gene knockout, pathogenicity, smy1 gene

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